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	Provedor de dados Electron. J. Biotechnol. (3) BABT (1) Autor Borzani,Walter (1) Chen,Fudi (1) Hou,Shixia (1) Jalalirad,Reza (1) Li,Hao (1) Mallu,Maheswara Reddy (1) Ronda,Srinivasa Reddy (1) Vemula,Sandeep (1) Xu,Zhihan (1) Yang,Dazuo (1) Mais... Palavra-chave Fed-batch fermentation (4) Antibody fragment (1) Artificial neural network (1) Biological activity (1) Biomass volume (1) Cell disruption (1) Cell lysis (1) Corrections calculation (1) Cross flow filtration (1) Escherichia coli (1) Fermentation parameters (1) General regression neural network (1) Iturin A (1) Medium aqueous phase volume (1) Periplasmic expression (1) Purification (1) Recombinant protein (1) Secondary structure (1) Support vector machine (1) Mais... Tipo do documento Journal article (3) Info:eu-repo/semantics/article (1) Ano 2016 (1) 2015 (1) 2013 (1) 2008 (1) País Chile (3) Brazil (1) Idioma Inglês (4)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 4 Primeira ... 1 ... Última Production, purification and characterization of recombinant human antithrombin III by Saccharomyces cerevisiae Electron. J. Biotechnol. Mallu,Maheswara Reddy; Vemula,Sandeep; Ronda,Srinivasa Reddy. Background: Antithrombin III (ATIII) is a protein that inhibits abnormal blood clots (or coagulation) by breaking down thrombin and factor Xa. ATIII helps to keep a healthy balance between hemorrhage and coagulation. The present work demonstrated the production, purification and characterization of recombinant human antithrombin (rhAT) from yeast Saccharomyces cerevisiae BY4741 was demonstrated. After expression of rhAT by S. cerevisiae, the biomass and rhAT concentration were analyzed through fed-batch fermentation process. Results: In fed-batch fermentation, the biomass (maximum cell dry weight of 11.2 g/L) and rhAT concentration (312 mg/L) of the expressed rhAT were achieved at 84 h of cultivation time. The maximum cell lysis efficiency (99.89%) was... Tipo: Journal article Palavras-chave: Biological activity; Cell lysis; Cross flow filtration; Fed-batch fermentation; Purification; Secondary structure. Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000400012 User-friendly optimization approach of fed-batch fermentation conditions for the production of iturin A using artificial neural networks and support vector machine Electron. J. Biotechnol. Chen,Fudi; Li,Hao; Xu,Zhihan; Hou,Shixia; Yang,Dazuo. Background In the field of microbial fermentation technology, how to optimize the fermentation conditions is of great crucial for practical applications. Here, we use artificial neural networks (ANNs) and support vector machine (SVM) to offer a series of effective optimization methods for the production of iturin A. The concentration levels of asparagine (Asn), glutamic acid (Glu) and proline (Pro) (mg/L) were set as independent variables, while the iturin A titer (U/mL) was set as dependent variable. General regression neural network (GRNN), multilayer feed-forward neural networks (MLFNs) and the SVM were developed. Comparisons were made among different ANNs and the SVM. Results The GRNN has the lowest RMS error (457.88) and the shortest training time (1... Tipo: Journal article Palavras-chave: Artificial neural network; Fed-batch fermentation; General regression neural network; Iturin A; Support vector machine. Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000400003 Production of antibody fragment (Fab) throughout Escherichia coli fed-batch fermentation process: Changes in titre, location and form of product Electron. J. Biotechnol. Jalalirad,Reza. Background: Recombinant proteins, including antibodies and antibody fragments, often contain disulfide bond bridges that are necessary for their folding, stability and function. Production of disulfide-bond-containing proteins in the periplasm of Escherichia coli has been very useful, due to unique characteristics of the periplasm, for obtaining fully active and correctly folded products and for alleviating downstream processing. Results: In this study, fed-batch cultivation of Escherichia coli (E. coli) for production of Fab D1.3, which is an anti-hen egg white lysozyme (HEWL) antibody fragment was carried out at 37ºC, and the bacterial cells were induced by adding 0.1 mM IPTG to the culture medium. Fermentor was sampled over the course of fermentation;... Tipo: Journal article Palavras-chave: Antibody fragment; Cell disruption; Escherichia coli; Fed-batch fermentation; Periplasmic expression; Recombinant protein. Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000300009 Calculation of fermentation parameters from the results of a fed-batch test taking account of the volume of biomass in the fermenting medium BABT Borzani,Walter. The volume of biomass in the fermenting medium may significantly affect the values of fermentation parameters calculated from the measured concentrations of the substrates and/or products. The corrections proposed in this paper should be evaluated and, depending on their magnitude, considered in order to obtain more representative results. A numerical example is presented. Tipo: Info:eu-repo/semantics/article Palavras-chave: Biomass volume; Corrections calculation; Fed-batch fermentation; Fermentation parameters; Medium aqueous phase volume. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300001 Registros recuperados: 4 Primeira ... 1 ... Última

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