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| Ren,Xueying; Zhang,Xuecheng; Sui,Zhenghong. |
| Genes differentially expressed between tetrasporophytes and female gametophytes of Gracilaria/Gracilariopsis lemaneiformis were isolated by suppression subtractive hybridization (SSH) and screened by dot-blot macro-arrays. Different expression profiles of selected clones based on the results of dot-blot macro-arrays were verified using virtual Northern blots. Totally, 14 phase relative cDNAs had been isolated and sequence identified. Among them, seven cDNAs were respectively homologous to crucial metabolic enzymes, Rab GTPase, RP42 homolog, and two hypothetical proteins, while the rest did not have significant hits in the databases examined. The results of virtual Northern blots revealed that 11 cDNAs were differentially expressed between the two samples,... |
| Tipo: Journal article |
Palavras-chave: Female gametophyte; Gracilaria/Gracilariopsis lemaneiformis; Suppression subtractive hybridization; Tetrasporophyte; Virtual Northern blot. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000200006 |
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| Moço,Maria C.C.; Mariath,Jorge E.A.. |
| The female gametophyte has a monosporic origin and a Polygonum type development. The female gametophyte growth consumes a large part of the neighboring nucellar cells and, in the micropylar region, part of the nucellar epidermis and internal integument. The mature gametophyte is composed of only four cells due to the ephemeral characteristic of the antipodals. The synergids are pear-shaped cells with the formation of filiform apparatus. Their nuclei lie in the micropylar region and large vacuoles in the chalazal region, while the egg cell presents an opposite polarization. The central cell accumulates starch grains and the two polar nuclei approach the egg. Occasional development of two gametophytes was recorded. The closest to the micropyle develops... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Adesmia; Embryo sac; Embryology; Female gametophyte; Leguminosae. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042004000200004 |
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