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Registros recuperados: 163
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Determinación bioquímica de la actividad enzimática de aminopeptidasa (tcAPE) y amplificación de fragmentos de ADNc de posibles genes TcAPE de Theobroma cacao L Colegio de Postgraduados
Aguilar Román, Lorena.
En el presente trabajo, se propuso realizar una determinación bioquímica de la actividad enzimática de aminopeptidasa (tcAPE) durante los procesos de germinación y fermentación de la semilla de T. cacao L., así como amplificar los extremos 3’ de fragmentos de cDNA de los posibles genes codificantes. El trabajo inició con la determinación de la actividad enzimática de tcAPE. Los resultados indicaron que la actividad enzimática de tcAPE, está presente durante el proceso de germinación de la semilla de T. cacao L., sin embargo, la síntesis de esta enzima se eleva durante la fase de crecimiento, misma que corresponde a la última etapa del proceso de germinación de la semilla de cacao criollo del genotipo almendra blanca. La actividad de tcAPE también se...
Tipo: Tesis Palavras-chave: Theobroma cacao L.; Aminopeptidasa; Proteasas; Germinación; Fermentación; Maestría; Producción Agroalimentaria en el Trópico; Aminopeptidase; Proteases; Germination; Fermentation.
Ano: 2008 URL: http://hdl.handle.net/10521/1575
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Utilización de un biofertilizante líquido en maíz (Zea mays L.) bajo condiciones del trópico húmedo. Colegio de Postgraduados
Alejandro Góngora, Antonio Okendo.
El objetivo de ésta investigación fue elaborar un biofertilizante líquido (Biol) fermentado anaeróbicamente, utilizando subproductos animales y vegetales, además de evaluar su eficacia en el cultivo de maíz, se contrasto con aplicaciones de fertilizante químico (manejo tradicional) y un biofertilizante comercial. Dado que no existe una dosis generada para aplicaciones con biofertilizantes se probaron tres concentraciones (5%, 10%, 15%) con el fin de obtener una dosis óptima preliminar de biofertilización del maíz. El experimento se estableció en un predio del Poblado C-41, Plan Chontalpa, Huimanguillo, Tabasco. Para la elaboración y obtención del biofertilizante liquido fermentado anaeróbicamente, se preparó un tanque de 200 litros donde se vertieron los...
Palavras-chave: Biodigestor; Biomasa; Fermentación; Biol; Biofertilizante; Biomass; Fermentation; Biofertilizer; Producción Agroalimentaria en el Trópico; Maestría.
Ano: 2012 URL: http://hdl.handle.net/10521/758
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Emisiones de metano generadas por excretas de animales de granja y contenido ruminal de bovino. Colegio de Postgraduados
Ramírez Hernández, Irma Fabiola.
Las emisiones de metano (CH4) de las actividades ganaderas, como un gas de efecto invernadero, son estimadas para el desarrollo de inventarios. Sin embargo el aprovechamiento de la producción del CH4 a partir de excretas de animales rumiantes y no rumiantes, para la generación de energía, requiere de establecer condiciones de temperatura y humedad para eficientar la mayor producción de este gas, compuesto del biogás producido por la actividad microbiana. En este estudio, se desarrollaron cuatro experimentos para cuantificar la producción de CH4, con la fermentación controlada, en un sistema biorreactor-trampa. En el 1er experimento, se compararon diferentes excretas de animales rumiantes: estiércol de ganado lechero y en engorda, excretas de cabras y...
Palavras-chave: Biogás; Metano; Excretas; Rumiantes; Fermentación; Biogas; Metane; Manure; Ruminants; Fermentation; Doctorado; Hidrociencias.
Ano: 2010 URL: http://hdl.handle.net/10521/282
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Fermentation optimization of maltose-binding protein fused to neutrophil-activating protein from Escherichia coli TB1 Electron. J. Biotechnol.
Lu,Jike; Song,Qi; Ji,Zhenyu; Liu,Xin; Wang,Ting; Kang,Qiaozhen.
Background The fermentation conditions of recombinant maltose-binding protein fused to neutrophil-activating protein (rMBP-NAP) of Helicobacter pylori were optimized from Escherichia coli TB1 with varying medium, inoculum age and size, time, inducer, pH and temperature in batch fermentation. Results It was revealed that the optimal conditions for the production of rMBP-NAP in shake flask were as follows: M9 medium (with 3% yeast extract powder added), inoculum age of 19 h, inoculum size of 6%, initial pH of 6.6, temperature of 37°C, and 0.7 mmoL/L IPTG inducted 21 h in a 50 mL/250 mL shake flask. The recombinant protein yield was increased from 59 to 592 mg/L after optimization. Fermentation process conducted in a 10 L fermenter with similar conditions...
Tipo: Journal article Palavras-chave: Escherichia coli; Fermentation; Optimization; Recombinant protein.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000400004
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Analysis of batch and repeated fedbatch productions of Candida utilis cell mass using mathematical modeling method Electron. J. Biotechnol.
Lin,Yutian; Liu,Guoli; Lin,Huibin; Gao,Ling; Lin,Jianqiang.
Background: Candida utilis is widely used in bioindustry, and its cell mass needs to be produced in a cost effective way. Process optimization based on the experimental results is the major way to reduce the production cost. However, this process is expensive, time consuming and labor intensive. Mathematical modeling is a useful tool for process analysis and optimization. Furthermore, sufficient information can be obtained with fewer experiments by using the mathematical modeling, and some results can be predicted even without doing experiments. Results: In the present study, we performed the mathematical modeling and simulation for the cell mass production of Candida utilis based on limited batch and repeated fedbatch experiments. The model parameters...
Tipo: Journal article Palavras-chave: Candida utilis; Fermentation; Genetic algorithm; Mathematical model.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000400002
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Bionanoparticles, a green nanochemistry approach Electron. J. Biotechnol.
Cauerhff,Ana; Castro,Guillermo R.
Background: In the past decade, considerable attention has been paid for the development of novel strategies for the synthesis of different kind of nano-objects. Most of the current strategies are usually working by the use physical or chemical principles to develop a myriad of nano-objects with multiple applications. Main fields of nanotechnology applications range from catalysis, micro- and nano-electronics (semiconductors, single electrons transistors), non-linear optic devices, photo-electrochemistry to biomedicine, diagnostics, foods and environment, chemical analysis and others. Results: Two main avenues for nanoparticles synthesis: cell-free extract and cell cultivation have been reported. The state of art of both biotechnological approaches for...
Tipo: Journal article Palavras-chave: Bacteria; Bionanoparticles; Fermentation; Fungi green chemistry; Plant extracts.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000300011
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Hyper-production of a thermotolerant β-xylosidase by a deoxy-D-glucose and cycloheximide resistant mutant derivative of Kluyveromyces marxianus PPY 125 Electron. J. Biotechnol.
Rajoka,Muhammad Ibrahim; Khan,Sana.
Production of β-xylosidaseby a cycloheximide and 2-deoxy-D-glucose-resistant mutant of Kluyveromyces marxianus PPY125 was studied when cultured on growth media containing galactose, glucose, xylose, cellobiose, sucrose and lactose as carbon sources. Xylose, cellobiose, lactose and sucrose were the key substrates. Both K. marxianus PPY125 and its mutant (M 125) supported maximum β-xylosidase specific product yield (Y P/X) following growth on xylose. Basal level of activity was observed in non-induced cultures grown on glucose. The mutant produced 1.5 to 2-fold more β-xylosidase than that produced by the wild cells. Synthesis of β-xylosidase was regulated by an induction mechanism in both wild and mutant cells. Addition of...
Tipo: Journal article Palavras-chave: Enthalpy; Entropy; Enzyme kinetics; Fermentation; Induction; Thermodynamics.
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200007
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Consolidated briefing of biochemical ethanol production from lignocellulosic biomass Electron. J. Biotechnol.
Spyridon,Achinas; Willem Euverink,Gerrit Jan.
Bioethanol production is one pathway for crude oil reduction and environmental compliance. Bioethanol can be used as fuel with significant characteristics like high octane number, low cetane number and high heat of vaporization. Its main drawbacks are the corrosiveness, low flame luminosity, lower vapor pressure, miscibility with water, and toxicity to ecosystems. One crucial problem with bioethanol fuel is the availability of raw materials. The supply of feedstocks for bioethanol production can vary season to season and depends on geographic locations. Lignocellulosic biomass, such as forest-based woody materials, agricultural residues and municipal waste, is prominent feedstock for bioethanol cause of its high availability and low cost, even though the...
Tipo: Journal article Palavras-chave: Bioconversion; Bioethanol; Fermentation; Hydrolysis; Renewable fuels.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000500009
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Bioconversion of mixed free fatty acids to poly-3-hydroxyalkanoates by Pseudomonas putida BET001 and modeling of its fermentation in shake flasks Electron. J. Biotechnol.
Makhdum Munawar,Khalil Munawar; Simarani,Khanom; Annuar,Mohamad Suffian Mohamad.
Background: The paper reports on the utilization of palm kernel oil (PKO) as a low cost renewable substrate for medium-chain-length poly-3-hydroxyalkanoates (mcl-PHA) production by Pseudomonas putida BET001. Investigation on the effects of selected key variables on growth, mixed free fatty acids consumption and mcl-PHA production by the bacterial culture in the shaken flask system were carried out along with its kinetic modeling. Results: The biomass production, fatty acids consumption and mcl-PHA production were found favorable when the strain was cultured in mineral medium at pH 6-7,28°C, aeration surface-to-volume ratio of 0.4 x 10(6) m-1, 250 rpm agitation rate for 48 h. Mcl-PHA production by this strain showed mixed growth and non-growth associated...
Tipo: Journal article Palavras-chave: Biopolymer; Fermentation; Medium-chain-length PHA; Palm kernel oil; Pseudomonas putida.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000100008
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1,3-Propanediol production from crude glycerol by Clostridium butyricum DSP1 in repeated batch Electron. J. Biotechnol.
Szymanowska-Powalowska,Daria.
Background The production of biofuels from renewable energy sources is one of the most important issues in industrial biotechnology today. The process is known to generate various by-products, for example crude glycerol, which is obtained in the making of biodiesel from rapeseed oil. Crude glycerol may be utilized in many ways, including microbial conversion to 1,3-propanediol (1,3-PD), a raw material for the synthesis of polyesters and polyurethanes. Results The paper presents results of a study on the synthesis of 1,3-propanediol from crude glycerol by a repeated batch method with the use of Clostridium butyricum DSP1. Three cycles of fermentation medium replacement were carried out. The final concentration of 1,3-PD was 62 g/L and the maximum...
Tipo: Journal article Palavras-chave: Environmental stress; Fermentation; High-performance liquid chromatography.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000600011
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Production of extracellular alkaline protease by new halotolerant alkaliphilic Bacillus sp. NPST-AK15 isolated from hyper saline soda lakes Electron. J. Biotechnol.
Ibrahim,Abdelnasser S.S; Al-Salamah,Ali A; Elbadawi,Yahya B; El-Tayeb,Mohamed A; Ibrahim,Shebl Salah Shebl.
Background Alkaline proteases are among the most important classes of industrial hydrolytic enzymes. The industrial demand for alkaline proteases with favorable properties continues to enhance the search for new enzymes. The present study focused on isolation of new alkaline producing alkaliphilic bacteria from hyper saline soda lakes and optimization of the enzyme production. Results A new potent alkaline protease producing halotolerant alkaliphilic isolate NPST-AK15 was isolated from hyper saline soda lakes, which affiliated to Bacillus sp. based on 16S rRNA gene analysis. Organic nitrogen supported enzyme production showing maximum yield using yeast extract, and as a carbon source, fructose gave maximum protease production. NPST-AK15 can grow over a...
Tipo: Journal article Palavras-chave: Alkaline protease; Alkalophiles; Bacillus sp; Fermentation; Optimization.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300015
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Potential use of Lactobacillus casei TISTR 1500 for the bioconversion from palmyra sap and oil palm sap to lactic acid Electron. J. Biotechnol.
Chooklin,Supasit; Kaewsichan,Lupong; Kaewsrichan,Jasadee.
Lactic acid is a product that finds several applications in food, cosmetic, pharmaceutical and chemical industries. The main objective of this work is to evaluate potential use of the sap from palmyra (Borassus flabellifer Linn.) and oil palm (Elaeis guineensis) as substrate for lactic acid production by Lactobacillus casei TISTR 1500. The effects of acid hydrolysis, pH control and nutrient supplement of palmyra sap and oil palm sap on fermentation performance were investigated. It was found that lactic acid fermentation using palmyra sap was not significantly affected by either acid hydrolysis or pH control. The addition of MRS increased biomass and product yield. The final lactic acid concentration, dry cell weight and productivity were increased by...
Tipo: Journal article Palavras-chave: Fermentation; Lactic acid; Lactobacillus casei TISTR 1500; Oil palm sap; Palmyra sap.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000500010
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Production and stability studies of the bioemulsifier obtained from a new strain of Candida glabrata UCP 1002 Electron. J. Biotechnol.
Asfora Sarubbo,Leonie; Moura de Luna,Juliana; de Campos-Takaki,Galba Maria.
Evaluation of both tenso-active and emulsifying activities indicated that a biosurfactant was produced by the newly isolated and promising strain Candida glabrata isolated from mangrove sediments. The extracellular water-soluble emulsifying agent was isolated and identified as a heteropolymer. The maximum of bioemulsifier production was observed when the strain was grown on soluble and insoluble substrates cotton seed oil plus glucose, reaching values of 10.0 g/l after 144 hrs at 200 rpm. The cell-free culture broth containing the examined agent lowered the surface tension of the medium to 31 mN/m. Stable and compact emulsions with emulsifying activity of 75% of cotton seed oil were detected. The emulsification capacity remained practically unaltered...
Tipo: Journal article Palavras-chave: Biosurfactant; Candida; Emulsifier; Fermentation; Glucose; Vegetal oil.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000400008
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Lagoas de estabilização natural para armazenamento de dejetos líquidos de suínos. Infoteca-e
KONZEN, E. A.; BARROS, L. C. de.
Desenvolvimento das lagoas de bioestabilizacao; Impermeabilizacao de lagoas com manta de plastico.
Tipo: Documentos (INFOTECA-E) Palavras-chave: Suinos; Dejetos; Manure.; Fermentação.; Fermentation; Swine; Wastes..
Ano: 1997 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/478844
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Kojic acid: Production of kojic acid from crude glycerol. Infoteca-e
DAMASO, M. C. T..
Kojic acid production using selected filamentous fungus isolated from the Brazilian biodiversity. The production is carried out in a submerged fermentation with crude glycerol (glycerin), a biodiesel co-product, or a commercial standard glycerol as the sole source of carbon.
Tipo: Folders Palavras-chave: Ácido kójico; Kojic acid; Fermentation; Glycerol.
Ano: 2021 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1132996
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Fermentação e secagem de amêndoas de cupuaçu para agroindústrias familiares. Infoteca-e
SOUZA, J. M. L. de; CARTAXO, C. B. da C.; ANDRADE NETO, R. de C.; MOURA, S. I. A.; MACIEL, V. T.; CRODA, J. P.; PIRES, F. C.; SARAIVA, L. S.; ARAÚJO, A. P..
As sementes do cupuaçu (Theobroma grandifl orum Schum (Willd. ex Spreng.) K. Schum.) possuem características botânicas e propriedades químicas similares às sementes do cacau (Theobroma cacao L.), e o seu processamento agrega valor à produção agroflorestal. Após os processos de fermentação e secagem das sementes recém-despolpadas pode-se produzir entre outros produtos o liquor, que é um análogo do chocolate e pode ser empregado na formulação de bolos, biscoitos e sorvetes, além da possibilidade de uso da gordura para fi ns alimentícios e cosméticos.
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E) Palavras-chave: Cupuassu; Almendras; Tecnología de procesamiento; Fermentación; Secado; Cupuaçu; Theobroma Grandiflorum; Amêndoa; Processamento; Fermentação; Secagem; Tecnologia de Alimento; Indústria Agrícola; Almonds; Processing technology; Fermentation; Drying; Agricultural industry.
Ano: 2018 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1091444
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Lignocellulosic biomass: Removal of fermentation inhibitors in lignocellulosic biomass liquors. Infoteca-e
RODRIGUES, C. M..
Processes for removal of fermentation inhibitors in lignocellulosic biomass liquors using medium pressure columns with regenerable polymeric/hydrophobic stationary phases and use of chemically green solvents such as water and ethanol.
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E) Palavras-chave: Sugarcane; Solvents; Fermentation; Renewable resources.
Ano: 2021 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1133085
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Acetoin: Production of acetoin from glycerin. Infoteca-e
SALUM, T. F. C..
Fermentative process for acetoin production from crude glycerin from biodiesel production using a new bacterial strain.
Tipo: Folders Palavras-chave: Biocombustível; Acetoin; Fermentation; Biodiesel.
Ano: 2021 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1132995
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Vinificação de uvas tintas | Vinificação de uvas brancas. Infoteca-e
BEN, R. L.; MONTEIRO, R.; DIDONE. T. B.; MANFROI, L..
Este folder informativo trata do processo de vinificação de uvas tintas e brancas, ou seja, o conjunto de operações técnicas que transformam as uvas em vinho. Aborda todas as fases do processo, deste a higienização de equipamentos e colheita das uvas até a filtração e o engarrafamento do vinho.
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E) Palavras-chave: Vinificação; Uvas tintas; Uvas brancas; Elaboração; Dtqprocessamento; Uva; Vinho; Colheita; Fermentação; Grapes; Wines; Winemaking; Wine grapes; Fermentation.
Ano: 2012 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1004464
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FERMENTAÇÃO positiva do café: "Sprouting Process". Infoteca-e
Este vídeo ensina como fazer a fermentação positiva do café com o método Sprouting Process.
Tipo: Dia de Campo na TV (INFOTECA-E) Palavras-chave: Sprouting process; Fermentação positiva; Embrapa Rondônia; Porto Velho (RO); Rondônia; Âmazônia Ocidental; Western Amazon; Café Robusta; Coffea Canephora; Coffee beans; Fermentation.
Ano: 2020 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1130939
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