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| Mello,M.R.B.; Caetano,H.V.A.; Marques,M.G.; Padilha,M.S.; Garcia,J.F.; Milazzotto,M.P.; Assumpção,M.E.O.A.; Lima,A.S.; Nicácio,A.C.; Mendes,C.M.; Oliveira,V.P.; Visintin,J.A.. |
| The present study examined the in vitro and in vivo development of bovine nuclear-transferred embryos. A bovine fetal fibroblast culture was established and used as nucleus donor. Slaughterhouse oocytes were matured in vitro for 18 h before enucleation. Enucleated oocytes were fused with fetal fibroblasts with an electric stimulus and treated with cytochalasin D and cycloheximide for 1 h followed by cycloheximide alone for 4 h. Reconstructed embryos were cultured for 7-9 days and those which developed to blastocysts were transferred to recipient cows. Of 191 enucleated oocytes, 83 (43.5%) were successfully fused and 24 (28.9%) developed to blastocysts. Eighteen freshly cloned blastocysts were transferred to 14 recipients, 5 (27.8%) of which were pregnant... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Cloning; Nuclear transfer; Bovine; Fetal fibroblasts; Embryo reconstruction. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100006 |
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| Felmer D,Ricardo; Arias,María Elena. |
| Since the first successful somatic cell nuclear transfer (SCNT) experiments were carried out, a number of domestic and agriculture species have been cloned using donor cells derived from different sources and origin. However, differences in nuclear transfer efficiency both in vitro and in vivo have been generally observed. These differences may be accentuated when transgenic cell lines are used as nuclear donors in an attempt to generate transgenic cloned offspring. The present study examined the suitability of cell lines derived from 3 different fetal sources and the effects of genetic manipulation of donor fetal fibroblasts with a red fluorescent plasmid, on the in vitro developmental potential and quality of nuclear transfer derived bovine embryos. We... |
| Tipo: Journal article |
Palavras-chave: Bovine embryos; Fetal fibroblasts; Nuclear transfer; Transfected cells. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000300005 |
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