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Nucleotide excision repair pathway assessment in DNA exposed to low-intensity red and infrared lasers BJMBR
Fonseca,A.S.; Campos,V.M.A.; Magalhães,L.A.G.; Paoli,F..
Low-intensity lasers are used for prevention and management of oral mucositis induced by anticancer therapy, but the effectiveness of treatment depends on the genetic characteristics of affected cells. This study evaluated the survival and induction of filamentation of Escherichia coli cells deficient in the nucleotide excision repair pathway, and the action of T4endonuclease V on plasmid DNA exposed to low-intensity red and near-infrared laser light. Cultures of wild-type (strain AB1157) E. coli and strain AB1886 (deficient in uvrA protein) were exposed to red (660 nm) and infrared (808 nm) lasers at various fluences, powers and emission modes to study bacterial survival and filamentation. Also, plasmid DNA was exposed to laser light to study DNA lesions...
Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA; Enzyme; Escherichia coli; Filamentation; Laser.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001000011
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Low-intensity red and infrared laser effects at high fluences on Escherichia coli cultures BJMBR
Barboza,L.L.; Campos,V.M.A.; Magalhães,L.A.G.; Paoli,F.; Fonseca,A.S..
Semiconductor laser devices are readily available and practical radiation sources providing wavelength tenability and high monochromaticity. Low-intensity red and near-infrared lasers are considered safe for use in clinical applications. However, adverse effects can occur via free radical generation, and the biological effects of these lasers from unusually high fluences or high doses have not yet been evaluated. Here, we evaluated the survival, filamentation induction and morphology of Escherichia coli cells deficient in repair of oxidative DNA lesions when exposed to low-intensity red and infrared lasers at unusually high fluences. Cultures of wild-type (AB1157), endonuclease III-deficient (JW1625-1), and endonuclease IV-deficient (JW2146-1) E. coli, in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA; Escherichia coli; Filamentation; Laser.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001000013
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Filamentous growth in Saccharomyces cerevisiae BJM
Ceccato-Antonini,Sandra Regina; Sudbery,Peter Edwin.
Fungal dimorphism is a complex phenomenon triggered by a large variety of environmental factors and consists of a reversible alternating pattern of growth between different elliptical and filamentous forms of cells. Understanding the mechanisms that regulate these events is of major interest because of their implications in fungal pathogenesis, cell differentiation and industry. Diploid cells of Saccharomyces cerevisiae transform from budding yeast to pseudohyphae when starved for nitrogen, giving the cells an advantage in food foraging, which is sensed by at least two signal transduction pathways: the MAP kinase (MAPK) and the PKA (cAMP-dependent protein kinase A) pathways. The output of these signalling pathways is the expression of pseudohypha-specific...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Filamentation; Saccharomyces cerevisiae; Pseudohyphae.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200001
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Vortex erosion in a shallow-water model ArchiMer
Louazel, Stephanie; Hua, Bach-lien.
The purpose of this paper is to study the erosion of eddies by filamentation in a shallow-water model where both fast and slow components of the dynamics coexist. In presence of inertia-gravity waves this filamentation process is indeed found to be altered. However, the information required to analyze the filamentation process is shown to be entirely captured by the slow component of the flow and regions of filament ejection can be identified from a diagnostic criterion based on instantaneous slow quantities. This is illustrated numerically in a reduced gravity model for an initial elliptic vortex of distributed potential vorticity in presence of Poincare waves. (C) 2004 American Institute of Physics.
Tipo: Text Palavras-chave: Numerical model; Filamentation; Vorticity; Eddies.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-711.pdf
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Influence des ondes d'inertie-gravité sur la dynamique tourbillonnaire ArchiMer
Louazel, Stephanie.
The purpose of this thesis is to study the influence of inertia-gravity waves on eddies dynamics, this process being part of the interaction between slow and fast dynamics of oceanic flows. Eddies dynamics is indeed slow but it is also intermediate between the fast inertia-gravity waves dynamics and the very low frequency dynamics of general circulation. The first part reviews the literature on this interaction. Then two numerical studies are performed. The first one concerns the influence of inertia-gravity waves on the filamentation diagnostic of an elliptic vortex. In presence of inertia-gravity waves this filamentation process is found to be slightly altered. However, the information required to analyze the filamentation process is shown to be entirely...
Tipo: Text Palavras-chave: Numerical experiment; Filamentation; Waves / vortex interaction; Modélisation numérique; Filamentation; Interaction ondes / tourbillons.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/these-539.pdf
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