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	Provedor de dados Anais da ABC (AABC) (1) BJMBR (1) Autor Carmona,Adriana K. (1) Jayaraman,V. (1) Juliano,Luiz (1) Juliano,Maria Aparecida (1) Mankiewicz,K.A. (1) Palavra-chave Fluorescence resonance energy transfer (2) A-Amino-3-hydroxy- 5-methyl-4- isoxazolepropionic acid receptors (1) Agonist activation (1) Angiotensin I-converting enzyme (1) Continuous recording assay (1) FRET substrates (1) Ligand binding (1) Neprilisin (1) Proteolytic enzymes (1) Vibrational spectroscopy (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2009 (1) 2007 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última The use of Fluorescence Resonance Energy Transfer (FRET) peptidesfor measurement of clinically important proteolytic enzymes Anais da ABC (AABC) Carmona,Adriana K.; Juliano,Maria Aparecida; Juliano,Luiz. Proteolytic enzymes have a fundamental role in many biological processes and are associated with multiple pathological conditions. Therefore, targeting these enzymes may be important for a better understanding of their function and development of therapeutic inhibitors. Fluorescence Resonance Energy Transfer (FRET) peptides are convenient tools for the study of peptidases specificity as they allow monitoring of the reaction on a continuous basis, providing a rapid method for the determination of enzymatic activity. Hydrolysis of a peptide bond between the donor/acceptor pair generates fluorescence that permits the measurement of the activity of nanomolar concentrations of the enzyme. The assays can be performed directly in a cuvette of the fluorimeter or... Tipo: Info:eu-repo/semantics/article Palavras-chave: Continuous recording assay; Fluorescence resonance energy transfer; FRET substrates; Proteolytic enzymes; Angiotensin I-converting enzyme; Neprilisin. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000300005 Glutamate receptors as seen by light: spectroscopic studies of structure-function relationships BJMBR Mankiewicz,K.A.; Jayaraman,V.. Ionotropic glutamate receptors are major excitatory receptors in the central nervous system and also have a far reaching influence in other areas of the body. Their modular nature has allowed for the isolation of the ligand-binding domain and for subsequent structural studies using a variety of spectroscopic techniques. This review will discuss the role of specific ligand:protein interactions in mediating activation in the a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid subtype of glutamate receptors as established by various spectroscopic investigations of the GluR2 and GluR4 subunits of this receptor. Specifically, this review will provide an introduction to the insight gained from X-ray crystallography and nuclear magnetic resonance investigations... Tipo: Info:eu-repo/semantics/article Palavras-chave: Vibrational spectroscopy; Fluorescence resonance energy transfer; A-Amino-3-hydroxy- 5-methyl-4- isoxazolepropionic acid receptors; Agonist activation; Ligand binding. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001100001 Registros recuperados: 2 Primeira ... 1 ... Última

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