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Recovery and characterisation of DNA from virgin olive oil Organic Eprints
Muzzalupo, PhD Innocenzo; Perri, Dr Enzo.
The characterisation of the genetic identity of DNA recoverable from olive oil could facilitate the assessment of its place of origin and conformity to type thanks to the particular regional spread of olive cultivars and to their different contributions to the olive oil mixture as stated by each Protected Designation of Origin regulation. This requires that intact DNA should be recovered from virgin olive oil. In an attempt to recover DNA from virgin olive oil, the performance of three different oil production methods was compared. The recorded data demonstrate that only olive oil obtained by using proteinase K treatment during the malaxation process contains DNA amenable to random amplified polymorphic DNA (RAPD)-PCR amplification. This important result...
Tipo: Journal paper Palavras-chave: Food security; Food quality and human health Olive Values; Standards and certification.
Ano: 2002 URL: http://orgprints.org/10217/1/EFRT2002.pdf
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DETERMINAZIONE INNOVATIVA DEL ROTENONE NEGLI OLI DI OLIVA DA AGRICOLTURA BIOLOGICA MEDIANTE SPETTROMETRIA DI MASSA TANDEM Organic Eprints
Caravita, M.A; Di Donna, L; Fusto, D; Mazzotti, F; Muzzalupo, I; Parise, A; Perri, E; Sindona, G.
Il rotenone, pesticida naturale utilizzato in agricoltura biologica su una grande varietà di colture, è stato determinato quantitativamente mediante Atmosheric Pressure Chemical Ionisation Spettrometria di Massa Tandem (APCI MS/MS) nelle olive e negli oli di oliva ottenuti dopo trattamento in una prova di campo che ha interessato la cv Carolea in Calabria. La tecnica analitica ha previsto la realizzazione di esperimenti “Multiple Reaction Monitoring” (MRM) utilizzando uno standard interno ottenuto per sintesi. Le quantità rilevate sono comprese tra 9 mg/Kg di olio dopo 1 giorno dal trattamento e 0.15 mg/Kg di olio dopo 1 mese circa dal trattamento. Quest’ultimo valore è molto più elevato dei 40 µg/Kg permessi dalla legislazione italiana.
Tipo: Conference paper, poster, etc. Palavras-chave: Food security; Food quality and human health Olive Values; Standards and certification.
Ano: 2004 URL: http://orgprints.org/10271/1/Rotenone_Olio_biologico.pdf
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RAPD-PCR amplification of DNA from virgin olive oil Organic Eprints
Perri, Dr Enzo; Muzzalupo, PhD Innocenzo; Sirianni, Ms Raffaella.
The characterization of the genetic identity of DNA recoverable from olive oil could facilitate the identification of the source cultivar. This requires that intact DNA or large fragments suitable for amplification by polymerase chain reaction (PCR) should be recovered from olive oil. This paper presents the preliminary results of an experiment evaluating the extent to which DNA recoverable from the veiled virgin olive (cv. Carolea) oil would allow PCR amplification useful for random amplified polymorphic DNA (RAPD) fingerprinting. Only residual DNA from olive oil sediments obtained by protease treatment was useful in RAPD-PCR amplification.
Tipo: Conference paper, poster, etc. Palavras-chave: Food security; Food quality and human health Olive Values; Standards and certification.
Ano: 2002 URL: http://orgprints.org/10236/1/Acta_Hort_2002.doc
Registros recuperados: 3
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