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Stable and efficient Agrobacterium tumefaciens mediated transformation of Phaseolus vulgaris Agrociencia
Espinosa-Huerta,Elsa; Quintero-Jiménez,Anareli; Cabrera-Becerra,K. Virginia; Mora-Avilés,M. Alejandra.
Common bean breeding requires novel techniques to incorporate genes that may not be available in bean genetic diversity. The objective of the present study was to analyze the main variables involved in Agrobacterium tumefaciens-mediated transformation of common bean towards providing an efficient system to introduce new agronomic traits. Bean cultivars Flor de Mayo Anita and Pinto Saltillo were inoculated with different A. tumefaciens strains, constructs and selection was done using two selection agents (kanamycin ot glufosinateammonium herbicide). Five-day old hypocotyls regenerated organogenic buds (cell clusters) 5 d after inoculation. A randomized complete blocks experimental design was used and the experimental unit was a petri dish with at least 10...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bud clusters; Drought stress tolerance; Fungal resistance; Gamborg medium; Hypocotyls; Organogenesis.
Ano: 2013 URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952013000400002
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Morphological, pathogenic and genetic diversity of Botrytis cinerea Pers. in blackberry cultivations in Colombia Rev. Bras. Frutic.
Isaza,Liliana; Zuluaga,Yenni Paola; Marulanda,Marta Leonor.
Abstract The Andean blackberry (Rubus glaucus Benth) is one of the most important fruits with the greatest commercial projection in Colombia. The factors that most affect its production are the attack of diseases and the scarce information about its control. One of the most limiting diseases of the crop is the gray mold, produced by the fungus Botrytis cinerea. This research presents the diversity of B. cinerea isolates from fields of andean blackberry in Colombia, based on the morphological, pathogenic and genetic characteristics allowing broadening the knowledge of the phytopathogen for subsequent management measures. Andean blackberry fruits were collected with characteristic symptoms of gray mold in farms located in eight producing areas of Colombian...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gray mold; Boty; Flipper; Fungal resistance.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000600401
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