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Chaparro, Cristian; Gayraud, Thomas; De Souza, Rogerio Fernandes; Domingues, Douglas Silva; Akaffou, Selastique; Laforga Vanzela, Andre Luis; De Kochko, Alexandre; Rigoreau, Michel; Crouzillat, Dominique; Hamon, Serge; Hamon, Perla; Guyot, Romain. |
A novel structure of nonautonomous long terminal repeat (LTR) retrotransposons called terminal repeat with GAG domain (TR-GAG) has been described in plants, both in monocotyledonous, dicotyledonous and basal angiosperm genomes. TR-GAGs are relatively short elements in length (<4 kb) showing the typical features of LTR-retrotransposons. However, they carry only one open reading frame coding for the GAG precursor protein involved for instance in transposition, the assembly, and the packaging of the element into the virus-like particle. GAG precursors show similarities with both Copia and Gypsy GAG proteins, suggesting evolutionary relationships of TR-GAG elements with both families. Despite the lack of the enzymatic machinery required for their mobility,... |
Tipo: Text |
Palavras-chave: Nonautonomous elements; LTR-retrotransposons; GAG; Conservation in plant genomes. |
Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00615/72715/71715.pdf |
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Delbarre-ladrat, Christine; Salas, Marcia Leyva; Sinquin, Corinne; Zykwinska, Agata; Colliec-jouault, Sylvia. |
Many bacteria biosynthesize structurally diverse exopolysaccharides (EPS) and excrete them into their surrounding environment. The EPS functional features have found many applications in industries such as cosmetics and pharmaceutics. In particular, some EPS produced by marine bacteria are composed of uronic acids, neutral sugars, and N-acetylhexosamines, and may also bear some functional sulfate groups. This suggests that they can share common structural features with glycosaminoglycans (GAG) like the two EPS (HE800 and GY785) originating from the deep sea. In an attempt to discover new EPS that may be promising candidates as GAG-mimetics, fifty-one marine bacterial strains originating from deep-sea hydrothermal vents were screened. The analysis of the... |
Tipo: Text |
Palavras-chave: Exopolysaccharides; GAG; Marine bacteria; Glycopolymers; Sulfate; Production. |
Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00399/51090/51873.pdf |
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Roger, Olivier; Colliec-jouault, Sylvia; Ratiskol, Jacqueline; Sinquin, Corinne; Guezennec, Jean; Fischer, A; Chevolot, Lionel. |
Three sulphated polysaccharides labelled at the reducing end with three different chemical treatments (2-aminopyridine (2-AP), 8-amino-1,3,6-trisulphonic acid (ANTS) and 6-(biotinyl)-aminocaproyl-hydrazide (BACH)) were compared. Molecular weight, chemical composition, nuclear magnetic resonance features and anticoagulant activity expressed as activated partial thromboplastin time were studied to determine whether structural and biological properties were retained or not after labelling. Partial depolymerisation and reduced biological activity were observed when reductive amination was performed with 2-AP. The initial properties were better retained with ANTS, and BACH did not modify the anticoagulant and structural properties. (C) 2002 Elsevier Science... |
Tipo: Text |
Palavras-chave: Anticoagulant activity; Reductive amination; Fluorescent tagging; Fucan; GAG; Sulphated polysaccharide. |
Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/2002/publication-2113.pdf |
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