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	Provedor de dados Ciência Rural (2) Autor Fajardo,Thor Vinícius Martins (2) Nickel,Osmar (2) Kin,Aléxis Cardama (1) Vanni,Marcos Fernando (1) Palavra-chave GRSPaV (2) Foveavirus (1) GLRaV-3 and -4 (1) GVA (1) GVD (1) Genetic variability (1) Lineages (1) RT-qPCR (1) Strains (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2017 (2) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Molecular variants of Grapevine rupestris stem pitting-associated virus infecting grapevines (Vitis spp.) in Brazil Ciência Rural Fajardo,Thor Vinícius Martins; Kin,Aléxis Cardama; Nickel,Osmar. ABSTRACT: Grapevine rupestris stem pitting-associated virus (GRSPaV) is one of the most common viruses of grapevine. It is involved in the graft-transmissible disease rupestris stem pitting of the rugose wood complex. The objective of the research was to perform the molecular characterization of the coat protein (CP) gene of sixteen Brazilian GRSPaV isolates aiming to determine the occurrence of molecular variants (strains) of this virus. Nine grapevine samples were evaluated, from which dsRNA was extracted. Nucleotide sequences were generated by Next generation sequencing (NGS). Fifteen complete sequences of the GRSPaV CP gene were obtained and phylogenetically analyzed. Multiple alignments of the sequences showed identities of nucleotides ranging from... Tipo: Info:eu-repo/semantics/article Palavras-chave: GRSPaV; Foveavirus; Strains; Lineages; Genetic variability. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001100153 Absolute quantification of viruses by TaqMan real-time RT-PCR in grapevines Ciência Rural Fajardo,Thor Vinícius Martins; Vanni,Marcos Fernando; Nickel,Osmar. ABSTRACT: The absolute quantification determines the absolute amount of a targeted nucleic acid expressed as a copy number or concentration. The knowledge of virus concentrations in commercial crops possesses high relevance to ensure a reliable diagnosis. The objective of this study was to perform an absolute quantification of five viruses in infected grapevines (Vitis spp.). Different known amounts of the standard sample (cloned viral cDNA or in vitro transcribed viral RNA) were quantified by TaqMan RT-qPCR. Based on these data, standard curves were generated plotting Ct values (threshold cycle) against the log of the standard sample amount. Infected grapevine samples were evaluated to determine virus titers, which were highly variable. This result may... Tipo: Info:eu-repo/semantics/article Palavras-chave: RT-qPCR; GRSPaV; GVA; GVD; GLRaV-3 and -4. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000600155 Registros recuperados: 2 Primeira ... 1 ... Última

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