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Production and characterization of galactose oxidase produced by four isolates of Fusarium graminearum BABT
Gasparotto,Elainy Patrícia Lino; Abrão,Sorrailla Carolina Carrard; Inagaki,Suely Yaeko; Tessmann,Dauri José; Kemmelmeier,Carlos; Tessmann,Ione Parra Barbosa.
A screening aimed to find new galactose oxidase producer isolates and to evaluate the production among Fusarium graminearum strains was conducted. Thirty-five isolates out of 39 analysed produced the enzyme at several levels. The data indicated a wide distribution of galactose oxidase within F. graminearum and also revealed new producer isolates. The enzyme produced by different isolates showed similar thermal activity and stability and were active on same substrates. However, the optimum pH ranged from 7.0 to 7.5. Thus, all evaluated isolates were suitable for the production of galactose oxidase.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Galactose oxidase; Fusarium graminearum; Enzyme.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500004
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A new PCR approach for the identification of Fusarium graminearum BJM
Biazio,Gleison Ricardo de; Leite,Gabriela Guimarães Sousa; Tessmann,Dauri José; Barbosa-Tessmann,Ione Parra.
The main objective of this work was to develop a PCR protocol for the identification of Fusarium graminearum, based on a pair of primers targeted to a segment of the 3' coding region of the gaoA gene that codes for the enzyme galactose oxidase (GO). This region has low homology with the same region of GO genes from other fungi. Genomic DNA from 17 strains of Fusarium spp. isolated from diseased cereals, from several other Fusarium species, and from other fungi genera was analyzed in a PCR assay using this primer set. The 17 strains of Fusarium spp. were also analyzed for the GO enzyme production in submerse fermentation in a new formulated liquid medium. All strains that were morphologically and molecularly identified as F. graminearum were able to secrete...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fusarium graminearum; Galactose oxidase; GaoA gene; Molecular identification.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300028
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Production of mycotoxins by galactose oxidase producing Fusarium using different culture BJM
Pereira,Angela Maria; Kemmelmeier,Carlos.
The original isolate of the galactose oxidase producing fungus Dactylium dendroides, and other five galactose oxidase producing Fusarium isolates were cultivated in different media and conditions, in order to evaluate the production of 11 mycotoxins, which are characteristic of the genus Fusarium: moniliformin, fusaric acid, deoxynivalenol, fusarenone-X, nivalenol, 3-acetyldeoxynivalenol, neosolaniol, zearalenol, zearalenone, acetyl T-2, and iso T-2. The toxicity of the culture extracts to Artemia salina larvae was tested.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Galactose oxidase; Fusarium; Dactylium dendroides; Mycotoxins.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000200013
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