| Gametogenic and histochemical aspects of an edible bivalve, lphigenia brasiliana (Lamarek, 1818) from Itaipu Lagoon, Niterói, State of Rio de Janeiro, were studied. Between May 1998 and October 1999 a total of 120 clams were picked up. Samples of 10 animals were collected once a month. Specimens were fixed in Bouin's fixative for 4 hours or less and transferred to 10% formalin for 24 hours. The shell lenght has been measured with the valves lightly opened to permit the penetration of fixative solution deeply in the tissues. Transverse sections including digestive diverticula, mantle, gills, gonad and foot were obtained from the total body. After fixative treatment, tissues sections were embedded in paraffin, cut routinely at 5 µm and stained with Harris's... |
