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Renner,Gladys Daniela Rogge; Randi,Áurea Maria. |
It is an endangered tree fern that grows in mixed umbrophylus forests in the tropics. Sterilized spores were germinated at 25 ± 2ºC under a 16-hour photoperiod, in Dyer and MS medium supplemented with 0 to 5% sucrose. The germination was lower with the addition of sucrose. Dry mass was higher in 30-day-old gametophytes cultivated in Dyer medium with the addition of 3 to 5% of sucrose. The dry mass decreased in 30-day-old gametophytes cultivated in Dyer and MS media without sucrose and in MS medium with the addition of 4 or 5% of sucrose. The effect of different irradiance on the germination and early gametophyte development of D. sellowiana was analyzed in the autumn of 1998 (May until July). Conical flasks containing spores were kept over a period of 49... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sugar; Chlorophyll; Gametophyte; Germination; Irradiance. |
Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062004000200017 |
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Luna,María L; Yañez,Agustina; Ramos Giacosa,Juan Pablo; Gorrer,Daniel; Berrueta,Pedro C; Giudice,Gabriela E. |
Anogramma chaerophylla belongs to a fern genus with annual sporophytes and potentially perennating gametophytes. In the studied area, Natural Reserve Punta Lara, the plants grow stressed mainly by pollution and the invasion of exotic species. As a part of a project on conservation of ferns inhabiting Buenos Aires province, the objectives of this work were to evaluate in Anogramma chaerophylla the conditions for in-vitro spore germination and to analyse the different stages of its reproductive cycle. Spores were sterilized in an aqueous solution 10 % of NaCIO during different times and then sown in Petri dishes with Murashige & Skoog medium, without the addition of sucrose. The dishes were kept under laboratory conditions, at 12 h light/ darkness... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anogramma chaerophylla; Micropropagation; Gametophyte; Tubercle; Ex-situ conservation. |
Ano: 2016 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-23722016000400010 |
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