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Correlation between chemical composition, kinetics of fermentation and methane production of eight pasture grasses Animal Sciences
Kulivand, Mahya; Kafilzadeh, Farokh.
Eight different grasses collected from pastures of the Kermanshah province (Kermanshah, Iran), at mid-vegetative stage were used to study the relationships between their chemical compositions, kinetic parameters of in vitro gas production and rumen methane production. There was a positive correlation (r = 0.62, p < 0.05) between crude protein (CP) content of grasses and total gas production (A) at 96h incubation. Negative correlations were also observed between acid detergent fiber (ADF) content and total gas production (r = -0.60, p < 0.05). Amongst the nutrients, neutral detergent fiber (NDF) and ADF were positively correlated with methane concentration, (r = 0.75 and 0.77, p < 0.01). The methane reduction potential (MRP) was negative for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gas production; Pasture grasses; Methane; In vitro.
Ano: 2015 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/24336
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Nutritional value of feed used in diets for Saanen goats Animal Sciences
Hygino, Bruna; Alcalde, Claudete Regina; Diaz, Tatiana Garcia; Olivo, Paula Martins; Santos, Geraldo Tadeu dos; Silva, Caroline Isabela da.
This study was performed to characterize feed used in diets for Saanen goats. The feedstuffs were: Tifton 85 hay (Cynodon spp.), soybean meal, ground corn, soybean hulls, ground ear corn and dried cassava residue. Contents were determined for dry matter, ash, crude protein, ether extract, neutral detergent fiber, acid detergent fiber, physically effective fiber and lignin, and organic matter, total carbohydrates and non-fiber carbohydrates were estimated. Carbohydrates were divided into fractions A + B1 (rapidly degradable), B2 (potentially degradable) and C (non-degradable); and the protein in fractions A (soluble), B1 (rapid ruminal degradation), B2 (intermediate degradation), B3 (slow degradation) and C (indigestible). To determine the gas production an...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.04.03.02-8 byproduct; Fractioning; Gas production; Goats; Ruminal degradation..
Ano: 2015 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/28705
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Nutritive value of Tifton 85 hay ammoniated with urea Animal Sciences
Almeida, João Carlos de Carvalho; Morais, Leonardo Fiusa de; Moreira, Thaís Gláucia Bueno; Abreu, João Batista Rodrigues de; Morenz, Mirton José Frota.
 The effect of ammonization was evaluated with urea at doses of 1, 2, 3 and 4%, on the basis of dry matter (DM) on Tifton 85 hay harvested at an advanced stage of development in the periods of 30 and 45 days, through analysis of dry matter, ash, ether extract (EE), neutral (NDF) and acid (ADF) detergent fiber, NDF corrected for ash and protein (NDFap), cellulose, lignin, crude protein (CP), acid detergent insoluble nitrogen (ADIN), fractionation of carbohydrates and in vitro gas production (Parameters: Vf1, k1, Vf2, k2 and L). The variables DM, EE, NDF, ADF, lignin, cellulose, NDFap, fraction A + B1, fraction B2 and C of carbohydrates, and the Vf1 and Vf2 parameters were influenced by treatments (p < 0.05). While DM, NDF, NDFap, fraction A + B1, C, CP...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zootecnia; Forragicultura; Tratamento de volumosos chemical treatment; Gas production; Low quality forages..
Ano: 2018 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/42473
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Fermentation kinetics and in vitro degradation rates of grasses of the genus Cynodon Animal Sciences
Reis, Sidnei Tavares dos; Lima, Marcus Vinícius Gonçalves; Sales, Eleuza Clarete Junqueira de; Monção, Flávio Pinto; Rigueira, João Paulo Sampaio; Santos, Leonardo David Tuffi.
The present study aimed to evaluate the fermentation kinetics and degradation rates of Cynodon grasses estimated by semi-automated technique of in vitro gas production. The forages were: Coastcross, Tifton 85 and Tifton 68. Pressure readings were taken at 0, 2, 4, 6, 8, 10, 12, 15, 19, 24, 30, 36, 48, 72 and 96 hours. Dry matter degradability (DMD) was obtained by the percentage of dry matter (DM) remaining after 0, 6, 12, 24, 48 and 96 hours of fermentation. Tifton 85 showed a higher total gas production (p <0.05). Higher fermentation rates were found at the beginning of fermentation followed by subsequent reduction (p <0.05) over time. Tifton 85 and Tifton 68 showed higher values (p < 0.05) for soluble fraction, potentially degradable insoluble...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.05.03.00-6 forage; Gas production; Semi-automated technique..
Ano: 2016 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/30009
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Chemical-bromatological composition and in vitro ruminal kinetics of sugar cane silage with maniçoba Animal Sciences
Carvalho, Francisco Allan Leandro de; Resende, Percivaldo Xavier; Benicio, Clístenes Amorim; Siqueira, Jackson de Oliveira; Menezes, Daniel Ribeiro; Queiroz, Mário Adriano Ávila.
The objective this study was to evaluate the effect of maniçoba supplementation in sugar cane silage with respect to chemical-bromatological composition and the in vitro degradation kinetics of the silage. This experiment was conducted in a completely randomized design with four treatments (maniçoba levels: 0, 20, 30, and 40%) and six repetitions. Silage samples were analyzed for their chemical-bromatological composition, digestible energy, metabolizable energy, total digestible nutrients, in vitro gas production and degradability parameters. The silage with higher inclusion level had better bromatological composition (p < 0.05) than the silage without maniçoba for CP, NDF, ADF and MM (6.49, 56.64, 38.66 and 4.52% versus 2.21, 70.96, 49.95 and 2.78%)....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Degradation; Forage conservation; Gas production; Saccharum officinarum..
Ano: 2018 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/42569
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In vitro evaluation of palm fronds as feedstuff on ruminal digestibility and gas production Animal Sciences
Khattab, Mostafa Sayed abd Ellatif; Abd El Tawab, Ahmed Mahmoud.
This study was carried out to evaluate using palm fronds only or supplemented with fibrolytic enzymes as alternative roughage on the ruminal nutrients digestibility and gas production. Treatments were: clover hay (Trifolium alexandrinum), palm fronds only and palm fronds plus fibrolytic enzymes (enzymes were mixed with palm fronds in rate of 4 g kg-1 of dry matter (DM). The results showed a reduction (p < 0.05) in gas production, ammoniac nitrogen (NH3-N, DM and organic matter (OM) digestibility in palm fronds compared with clover hay. While supplementing palm fronds with fibrolytic enzymes improved DM and OM digestibility compared with palm fronds only without (p < 0.05) differences with clover hay. Also, supplementing palm fronds with fibrolytic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ruminant nutrition fibrolytic enzymes; Gas production; Rumen; Nutrients digestibility..
Ano: 2018 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/39586
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Cinética da fermentação in vitro do capim-Marandu em diferentes idades de corte - doi: 10.4025/actascianimsci.v33i3.9462 Animal Sciences
Sá, Jacqueline Firmino de; Universidade Estadualdo Sudoeste da Bahia; Pedreira, Márcio dos Santos; UESB; Silva, Fabiano Ferreira da; UESB; Figueiredo, Mauro Pereira de; UESB; Rebouças, Gesiane Moura Neves; UFMG; Souza, Danilo Ribeiro de; UESB.
O objetivo do trabalho foi avaliar os parâmetros da cinética de degradação ruminal da Brachiaria brizantha cv. Marandu, cortadas aos 28, 35 e 54 dias, pela técnica in vitro de produção de gases. Foram realizadas determinações dos teores de matéria seca (MS), matéria orgânica (MO), proteína bruta (PB), extrato etéreo (EE), fibra em detergente neutro corrigida para cinzas e proteína (FDNCP), fibra em detergente ácido (FDA), nitrogênio insolúvel em detergente neutro (NIDN), nitrogênio insolúvel em detergente ácido (NIDA), lignina (LIG), Carboidratos totais (CT), carboidratos não fibrosos (CNF), fração B2 e fração C, respectivamente. Os dados da produção de gases foram ajustados ao modelo logístico bicompartimental. O delineamento utilizado foi inteiramente...
Tipo: Pesquisa de campo; Analise laboratorial Palavras-chave: 5.04.03.00-1 Brachiaria brizantha; Taxa de degradação; Produção de gás Nutrição e Alimentação Animal Brachiaria brizantha; Degradation rate; Gas production.
Ano: 2011 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/9462
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Use of F57 bags by the semi-automatic in vitro technique of gas production Arq. Bras. Med. Vet. Zootec.
Amaral,A.P.S.F.; Coelho,S.G.; Tomich,T.R.; Maurício,R.M.; Gonçalves,L.C.; Rocha e Silveira,S.; Pires Neto,O.S.; Rodrigues,J.A.S..
Os cadinhos filtrantes (porosidade 1) originalmente usados na técnica in vitro semi-automática de produção de gases estabelecida no Laboratório de Produção de Gases da Escola de Veterinária da UFMG podem ocasionar interferências nos estudos de quantificação da degradação de alimentos, principalmente os ricos em amido, devido à obstrução dos poros da placa filtrante pelo material residual após fermentação. Este estudo objetivou avaliar a substituição dos cadinhos filtrantes por sacos F57 (Ankom(r)) visando a realização de experimentos in vitro para descrição da cinética de fermentação e degradação ruminal. Esta substituição causou a redução do peso/volume da amostra incubada e desta forma, interferiu na relação pressão (P) / volume (V) e consequentemente...
Tipo: Info:eu-repo/semantics/article Palavras-chave: In vitro; Forage evaluation; F57 bags; Gas production.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000601936
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Relationship between chemical components, bacterial adherence and in vitro fermentation of tropical forage legumes Ciência e Agrotecnologia
Fluck,Ana Carolina; Kozloski,Gilberto Vilmar; Martins,Andressa Ana; Mezzomo,Mariana Patricia; Zanferari,Filipe; Stefanello,Simone.
Inclusion of forage legumes in diet may improve tropical ruminant systems productivity and sustainability. However, it is not well stablished which chemical components more impact their nutritional value. The relationship between chemical composition and in vitro fermentation of tropical forage legume was evaluated with the objective of obtaining indicators of their nutritional value. Samples of Crotalaria spectabilis, Cajanus cajan, Macrotyloma axillare, Mucuna aterrina, Stylosantis sp. and Canavalia ensiformis, cut from plants at growth age between 47 to 110 days, were analysed. Total gas production showed negative correlation (P<0.05) with total N (R=-0.51), acid detergent fibre (ADF, R=-0.62) and acid detergent lignin (ADL, R=-0.65), and positive...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gas production; Lignin; Nutritional value; Phenolic compounds; Tannins.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542013000500010
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Estudio del cultivo sólido de paja de sorgo en la producción de enzimas fibrolíticas y digestibilidad ruminal in vitro con Fomes fomentarius EUM1 y Pleurotus sapidus. Colegio de Postgraduados
Villegas Castañeda, Marcela.
Se estudió el cultivo sólido con Fomes fomentarius EUM1 y Pleurotus sapidus en paja de sorgo, con un tratamiento alcalino y húmedo. El cultivo se realizó durante 20 días previo tratamiento con humedad y Ca OH2 por 24 h. Se midió la producción de enzimas celulasas, xilanasas y lacasas (complejos lignocelulósicos) producidas en los días 0, 9,15 y 20 de fermentación Se encontró una actividad máxima de celulasas para el hongo F. fomentarius EUM1 de 15.24 ±0.99 U/gss y para P. sapidus de 6. 51±0.81 U/gss. La máxima actividad xilanasas en F. fomentarius fue 79.45±8.69 U/gss y para Pleurotus 24.90±0.71 U/gss. También se midió la proteína soluble, la mayor cantidad se cuantificó al día 20. Los cambios en la composición nutricional de la paja variaron paralelamente...
Palavras-chave: Cultivo sólido; Paja de sorgo; Pleurotus sapidus; Fomes fomentarius; Producción de gas; Solid culture; Fomes fomentarius; Gas production; Sorghum straw; Maestría; Ganadería.
Ano: 2010 URL: http://hdl.handle.net/10521/103
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Digestibilidad in vitro de dietas energéticas para ovinos con inclusión de semilla de girasol. Colegio de Postgraduados
Herrera Pérez, Jerónimo.
Cinco dietas integrales isoenergéticas e isoprotéicas fueron evaluadas con el objetivo de medir la producción de gas in vitro y asociarla con la digestibilidad de la materia seca en dietas para corderos elaboradas con 0, 3, 6, 9 y 12 % de inclusión de semilla de girasol. Se midió la producción de gas in vitro (PGSIV) (volumen máximo de gas (Vmax; mL g-1) para materia seca (MS), materia orgánica (MO) y materia seca degradada (MSD), la digestibilidad in vitro de la MS (DIVMS), y producción de ácidos grasos volátiles (AGV) a las 6, 12, 24, 48 y 72 h de incubación. El diseño experimental fue completamente al azar con bloques generalizados utilizando el procedimiento MIXED del SAS. Se realizaron polinomios ortogonales para probar el efecto lineal y cuadrático...
Palavras-chave: Acidos grasos volátiles; Producción de gas; Oleaginosas; Volatile fatty acids; Gas production; Oilseeds; Ganadería; Maestría.
Ano: 2013 URL: http://hdl.handle.net/10521/1952
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Estudio del cultivo sólido de paja de sorgo en la producción de enzimas fibrolíticas y digestibilidad ruminal in vitro con Fomes fomentarius EUM1 y Pleurotus sapidus. Colegio de Postgraduados
Villegas Castañeda, Marcela.
Se estudió el cultivo sólido con Fomes fomentarius EUM1 y Pleurotus sapidus en paja de sorgo, con un tratamiento alcalino y húmedo. El cultivo se realizó durante 20 días previo tratamiento con humedad y Ca OH2 por 24 h. Se midió la producción de enzimas celulasas, xilanasas y lacasas (complejos lignocelulósicos) producidas en los días 0, 9,15 y 20 de fermentación Se encontró una actividad máxima de celulasas para el hongo F. fomentarius EUM1 de 15.24 ±0.99 U/gss y para P. sapidus de 6. 51±0.81 U/gss. La máxima actividad xilanasas en F. fomentarius fue 79.45±8.69 U/gss y para Pleurotus 24.90±0.71 U/gss. También se midió la proteína soluble, la mayor cantidad se cuantificó al día 20. Los cambios en la composición nutricional de la paja variaron paralelamente...
Palavras-chave: Cultivo sólido; Paja de sorgo; Pleurotus sapidus; Fomes fomentarius; Producción de gas; Solid culture; Fomes fomentarius; Gas production; Sorghum straw; Maestría; Ganadería.
Ano: 2010 URL: http://hdl.handle.net/10521/103
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Effect of a simulated heat wave in thermal and aerial environment broiler-rearing environment REA
Vale,Marcos M. do; Moura,Daniella J. de; Nääs,Irenilza de A.; Curi,Thayla M. R. C.; Lima,Karla A. O..
ABSTRACT Global warming increases the occurrence of events such as extreme heat waves. Research on thermal and air conditions affecting broiler-rearing environment are important to evaluate the animal welfare under extreme heat aiming mitigation measures. This study aimed at evaluating the effect of a simulated heat wave, in a climatic chamber, on the thermal and air environment of 42-day-old broilers. One hundred and sixty broilers were housed and reared for 42 days in a climatic chamber; the animals were divided into eight pens. Heat wave simulation was performed on the 42nd day, the period of great impact and data sampling. The analyzed variables were room and litter temperatures, relative humidity, concentrations of oxygen, carbon monoxide and ammonia...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ammonia; Gas production; Animal welfare; Oxygen; Litter temperature.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69162016000200271
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Methane hydrate-bearing sediments: Pore habit and implications ArchiMer
Terzariol, Marco; Park, Junghee; Castro, Gloria M.; Santamarina, J. Carlos.
Hydrate-bearing sediments are relevant to the organic carbon cycle, seafloor instability, and as a potential energy resource. Sediment characteristics affect hydrate formation, gas migration and recovery strategies. We combine the physics of granular materials with robust compaction models to estimate effective stress and capillary pressure in order to anticipate the pore habit of methane hydrates as a function of the sediment characteristics and depth. Then, we compare these results to an extensive database of worldwide hydrate accumulations compiled from published studies. Results highlight the critical role of fines on sediments mechanical and flow properties, hydrate pore habit and potential production strategies. The vast majority of hydrate...
Tipo: Text Palavras-chave: Hydrate accumulation database; Gas production; Methane hydrate pore habit; Revised soil classification system.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00609/72158/70924.xml
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Nutritional divergence in genotypes of forage peanut R. Bras. Zootec.
Ferreira,Alexandre Lima; Maurício,Rogério Martins; Pereira,Luiz Gustavo Ribeiro; Azevêdo,José Augusto Gomes; Oliveira,Leandro Silva; Pereira,José Marques.
The objective of this study was to evaluate the nutritional divergence between ten genotypes of forage peanut, based on chemical composition as well as fermentation and in vitro degradation kinetic characteristics. Treatments consisted of ten genotypes of Arachis pintoi, namely eight accessions (31135, 30333, 15121, 31828, 15598, 31534, 13251 and 31496) and two cultivars (cv. Belmonte and cv. Amarillo). The genotypes were harvested in each plot at a height of 3 cm from the ground, in 42-day intervals, during the time of heaviest rainfall. For the multivariate analysis the following variables, the following were used: crude protein, neutral detergent fiber, potential degradation in 48 hours, degradation rate of insoluble potentially degradable fraction and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chemical composition; Gas production; Multivariate analysis.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012000400005
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In situ and in vitro degradation kinetics and prediction of the digestible neutral detergent fiber of agricultural and agro-industrial byproducts R. Bras. Zootec.
Azevêdo,José Augusto Gomes; Valadares Filho,Sebastião de Campos; Detmann,Edenio; Pina,Douglas dos Santos; Paulino,Mário Fonseca; Valadares,Rilene Ferreira Diniz; Pereira,Luiz Gustavo Ribeiro; Lima,Jéssika Carolina Moutinho.
The objective of this study was to evaluate the in situ and in vitro degradation kinetics and to predict the digestible neutral detergent fiber (dNDF) from the incubation times; in situ and in vitro degradation kinetic parameters; and equations fitted for agricultural and agro-industrial byproducts. Byproducts from pineapple, cocoa, palm kernel, corn gluten meal, common bean, sunflower, guava, cassava bark, cassava stems, cassava foliage, papaya, mango, passion fruit and turnips were evaluated. There were differences between the byproducts as for the potentially neutral detergent fiber (NDF) fraction and the in situ NDF degradation rate in the final volume of the gases generated by fibrous carbohydrates (FC), for the lag time and for the in vitro...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fibrous carbohydrates; Gas production; Residues; Rumen degradability.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012000800013
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Equine fecal inoculum optimization in in vitro fermentation assays of dehydrated roughage R. Bras. Zootec.
Franzan,Bruna Caroline; Franco,Tatiana Werneck; Stefani,Giselle; Pereira,Marcelo Maia; Almeida,Fernando Queiroz de; Silva,Vinicius Pimentel.
ABSTRACT This study evaluated the influence of coastcross hay substrate hydration and equine fecal inoculum dilution on the parameters of fermentation and microbiology in in vitro essays. A 2 × 2 factorial block design was used. The first factor was hydration of the coastcross hay substrate 12 h before incubation or at the time of incubation, and the second factor was the dilution of fecal inoculum with a nutrient solution in a weight: weight ratio of 1:1 or 1:3. Degradation of the dry matter (DM), organic matter (OM), and neutral detergent fiber (NDF) were evaluated at 24, 48, and 72 h. Microorganisms were counted 24 h after inoculation. The ammoniacal nitrogen concentration (NH3-N), pH, and cumulative gas production were measured up to 72 h and adjusted...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Degradation rate; Gas production; Microbiology; Modeling; Roughage.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100531
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Additives on in vitro ruminal fermentation characteristics of rice straw R. Bras. Zootec.
Peripolli,Vanessa; Barcellos,Júlio Otávio Jardim; Prates,Ênio Rosa; McManus,Concepta; Stella,Laion Antunes; Camargo,Cláudia Medeiros; Costa Jr,João Batista Gonçalves; Bayer,Cimélio.
ABSTRACT The objective of this study was to evaluate the effects of mineral and protein-energy (MPES), exogenous fibrolytic enzyme supplements (ES), combination of MPES + ES, and straw without supplement (WS) on digestibility, fermentation kinetic parameters, cumulative gas production, methane, CO2 production, and volatile fatty acid concentration of rice straw of low and high nutritional value, estimated by in vitro techniques. The experimental design was randomized and factorial 2 × 4: two straws (low and high nutritional value) incubated with four supplements (MPES, ES, MPES + ES, and WS) and their interactions. Four experimental periods were used, totaling four replications per treatment over time. Data were analyzed by PROC MIXED of SAS. The in vitro...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Digestibility; Digestion kinetics; Fatty acid; Gas production; Methane.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982017000300240
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In vitro fermentation characteristics of ruminant diets using ethanol extract of brown propolis as a nutritional additive R. Bras. Zootec.
Gomes,Maria de Fátima Falcão; Ítavo,Camila Celeste Brandão Ferreira; Ítavo,Luís Carlos Vinhas; Leal,Cássia Rejane Brito; Silva,Jonilson Araújo da; Heimbach,Natália da Silva; Leal,Eduardo Souza.
ABSTRACT The addition of levels of ethanol extract of brown propolis was evaluated by assessing diet degradation in rumen fluid and predicting cumulative in vitro gas production by nonlinear (dual pool logistic and exponential) models. A total of 35 g of crude propolis were extracted in 65 mL of cereal alcohol (95% ethanol). In a completely randomized factorial design, the experimental diets combined four concentrations of extracted propolis diluted in cereal alcohol (0, 50, 70, and 100% of propolis extract) and supplementation doses (4, 8, 12, 16, and 20 mL/kg dry matter), tested in triplicate. Diet (400 g/kg Tifton hay and 600 g/kg concentrate) was incubated for 96 h carried out three times in three different weeks. There was significant interaction...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Feed additive; Gas production; Propolis; Ruminal degradability; Ruminant nutrition.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982017000700599
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Methane production of two roughage and total mixed ration as influenced by cellulase and xylanase enzyme addition Scientia Agricola
Gemeda,Belete Shenkute; Hassen,Abubeker.
In recent decades supplementation of animal feeds with exogenous fibrolytic enzymes has substantially improved digestibility and animal performance. However, information related to associated methane production is limited and inconsistent. This study evaluated the effect of cellulase and xylanase enzymes on in vitro methane production of Eragrostis curvula hay, maize (Zea mays) stover and a total mixed ration (TMR) at seven levels of the two enzymes. Feed samples were incubated for 2, 12, 24 and 48 h in an in vitro batch culture with buffer and rumen fluid, and fibrolytic enzymes. Gas production was measured using a pressure transducer connected to a data tracker, while methane gas was analysed using a gas chromatograph which was calibrated with standard...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Digestibility; Fermentation; Fibrolyitc enzymes; Gas production.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000100011
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