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Thermostabilisation of human serum butyrylcholinesterase for detection of its inhibitors in water and biological fluids BABT
Jaganathan,Lakshmanan; Boopathy,Rathanam.
The ability of gelatine-trehalose to convert the normally fragile, dry human serum BChE into a thermostable enzyme and its use in the detection of cholinesterase inhibitors in water and biological fluids is described. Gelatine or trehalose alone is unable to protect the dry enzyme against exposure to high temperature, while a combination of gelatine and trehalose were able to protect the enzyme activity against prolonged exposure to temperature as high as +50°C. A method for rapid, simple and inexpensive means of screening for cholinesterase inhibitors such as carbamates and organophosphates in water, vegetables and human blood has been developed.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Butyrylcholinesterase; Carbamates; Cholinesterase; Gelatine; Organophosphorus; Trehalose.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300012
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Soft Collagen-Gelatine Sponges by Convection Drying BABT
Meyer,Michael; Trommer,Kristin.
This study showed that thermally labile fibrillar collagen could be processed continuously in combination with gelatine as foaming additive by convection drying. The procedure led to stable sponges with similar structural and physical properties as found for freeze-dried collagen samples. The fibrillar collagen remained native, while gelatine acted as foaming additive. The absorbency of the sponges was improved by opening the surface with abrasives. A use as medical device with hemostyptic properties would be possible.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Collagen; Gelatine; Convection drying; Sponge.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100109
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