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Comparative genomics reveals a widespread distribution of an exopolysaccharide biosynthesis gene cluster among Vibrionaceae ArchiMer
Lebellenger, Lou; Verrez-bagnis, Veronique; Passerini, Delphine; Delbarre Ladrat, Christine.
Objectives The eps locus in Vibrio diabolicus is involved in the production of the biotechnologically valuable HE800 EPS. In this study, the distribution and diversity of similar eps gene clusters across Vibrionaceae and its variability in relation to phylogenetic relationship were investigated. The aim was to provide a better knowledge of the eps gene cluster importance and to facilitate discovery of new EPS with potent interesting bioactivities. Results Seventy percent of the 103 genome sequences examined display such an eps locus with a high level of synteny. However, genetic divergence was found inside some monophyletic clades or even between some strains of the same species. It includes gene insertions, truncations, and deletions. Comparative analysis...
Tipo: Text Palavras-chave: Vibrionaceae; Exopolysaccharide; Genetic biodiversity; Biosynthesis; Vibrio diabolicus; HE800 EPS.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00424/53536/54392.pdf
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Genetic analyses of Rhizoctonia solani isolates from Phaseolus vulgaris grown in the Atlantic Rainforest Region of São Paulo, Brazil Trop. Plant Pathol.
MEINHARDT,LYNDEL W.; WULFF,NELSON A.; BELLATO,CLÁUDIA M.; TSAI,SIU M..
Rhizoctonia solani isolates obtained from common beans (Phaseolus vulgaris) grown in the mountainous Atlantic Rainforest (Mata Atlântica) region of São Paulo, Brazil, were analyzed to determine their genetic diversity using internal transcribed spacer (ITS), microsatellite and telomere sequence-based PCR primers. Restriction digestion of the ITS1/5.8S/ITS2 ribosomal regions yielded unique banding patterns specific for AG4 and its subgroups. The ITS restriction digestion (ITS/RFLP), telomere and microsatellite primers identified five to 11 genotypes within the isolates of R. solani. While all isolates were pathogenic on beans, there was no correlation found between genotypic differences and pathogenicity. The different PCR primers revealed a number of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mata Atlântica; Genetic biodiversity; Soil-borne fungi; ITS; Telomere.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582002000300004
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