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Registros recuperados: 73
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Genetic diversity in oil palm (Elaeis guineensis Jacq) using RAM (Random Amplified Microsatellites) Bragantia
Cardona,Christian Camilo Castañeda; Coronado,Yacenia Morillo; Conronado,Ana Cruz Morillo; Ochoa,Iván.
ABSTRACT The oil palm (Elaeis guineensis Jacq) plays an important role in the economy of some tropical countries; Colombia ranks first for production in Latin America and fifth in the world. The aim of this research was to characterize the genetic variability of 51 oil palm genotypes from the Congo with seven randomly amplified microsatellite markers RAM. As a result, 241 alleles were generated andthe number of polymorphic loci ranged from 46 to 14 for the ACA and CGA primers, respectively. High genetic diversity was found, with a total heterozygosity of 0.64, along with a high percentage of polymorphic loci, 89%. The coefficient of genetic differentiation (Fst) was 0.15, indicating that there was moderate genetic differentiation. The genetic diversity...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Breeding; Genetic markers; Germplasm; Genetic structure.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052018000400546
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Novel Microsatellite Markers for Brazilian Mangrove Oysters ( Crassostrea gasar ) and their Cross-Amplification in Crassostrea rhizophorae BABT
Baldez,Renata do Socorro Corrêa; Melo,Mauro André Damasceno; Sampaio,Iracilda; Tagliaro,Claudia Helena.
ABSTRACT A microsatellite CT/GT enriched genomic library was developed for Crassostrea gasar and twelve new polymorphic loci were isolated and characterized. The markers were successfully amplified from 25 individuals of Crassostrea gasar and 11 cross-amplified individuals of Crassostrea rhizophorae. There was no evidence of linkage between loci in either species.
Tipo: Info:eu-repo/semantics/article Palavras-chave: SSR; Genetic markers; Cupped oyster; Population structure..
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100426
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Distribution of HLA-DRB1 alleles in a mixed population with insulin-dependent diabetes mellitus from the Southeast of Brazil BJMBR
Marques,S.B.D.; Volpini,W.; Caillat-Zucman,S.; Lieber,S.R.; Pavin,E.J.; Persoli,L.B..
HLA class II genes are strongly associated with susceptibility and resistance to insulin-dependent diabetes mellitus (IDDM). The present study reports the HLA-DRB1 genotyping of 41 IDDM patients and 99 healthy subjects from the Southeast of Brazil (Campinas region). Both groups consisted of an ethnic mixture of Caucasian, African Negro and Amerindian origin. HLA-DRB1*03 and *04 alleles were found at significantly higher frequencies among IDDM patients compared to the controls (DRB1*03: 48.8% vs 18.2%, P<0.005, RR = 4.27; DRB1*04: 43.9% vs 15.1%, P<0.008, RR = 4.37) and were associated with a susceptibility to the disease. DRB1*03/*04 heterozygosity conferred a strong IDDM risk (RR = 5.44). In contrast, the HLA-DRB1*11 allele frequency was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Insulin-dependent diabetes mellitus; Genetic markers; DNA typing; HLA-DRB1.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000300005
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Isolation of (GA)n microsatellite sequences and description of a predicted MADS-box sequence isolated from common bean (Phaseolus vulgaris L.) Genet. Mol. Biol.
Yaish,M.W.F.; Pérez de la Vega,M..
The isolation of (GA)n microsatellites using a highly microsatellite-enriched library is described for the first time in common bean (Phaseolus vulgaris L.). A relatively simple and effective method to isolate DNA repeats from microsatellite-enriched libraries based on hybridization-capture of repeat regions using biotin-conjugated oligonucleotids and non-radioactive colony hybridization was carried out. PCR products from 200 to 800 bp were obtained and cloned. Of the 60 clones sequenced, 21 yielded (GA)n microsatellites with n values equal or higher than six. These (GA)n microsatellite-containing loci could be useful for further genetic mapping studies. A (GA)n microsatellite linked to a putative MADS-box gene was identified. This sequence, which...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phaseolus vulgaris L.; Genetic markers; Microsatellites; MADS-box; Transcriptional factor.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300019
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Combined use of mitochondrial and nuclear genetic markers further reveal immature marine turtle hybrids along the South Western Atlantic Genet. Mol. Biol.
Brito,Cíntia; Vilaça,Sibelle Torres; Lacerda,Ana Luzia; Maggioni,Rodrigo; Marcovaldi,Maria Ângela; Vélez-Rubio,Gabriela; Proietti,Maíra Carneiro.
Abstract Marine turtle hybridization is usually sporadic and involves reports of only a few individuals; however, Brazilian populations have high hybridization rates. Here we investigated the presence of hybrids in morphologically identified immature hawksbills (Eretmochelys imbricata) along the South Western Atlantic (SWA). We sequenced one mitochondrial (D-Loop) and three nuclear DNA (RAG1, RAG2, and CMOS) markers to better understand the patterns and characteristics of hybrids. We identified 22 hybrids (n = 270), 11 of them at the extreme South of the SWA. Uruguay had the highest hybrid frequency in the SWA (~37.5%) followed by southern Brazil with 30%. These are common areas for loggerheads (Caretta caretta) but uncommon for hawksbills, and these...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hybridization; Cheloniidae; Genetic markers; Hybrid distribution; Conservation.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400704
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Satellyptus: analysis and database of microsatellites from ESTs of Eucalyptus Genet. Mol. Biol.
Ceresini,Paulo Cezar; Silva,Cristina Lacerda Soares Petrarolha; Missio,Robson Fernando; Souza,Elaine Costa; Fischer,Carlos Norberto; Guillherme,Ivan Rizzo; Gregorio,Ivo; Silva,Eloiza Helena Tajara da; Cicarelli,Regina Maria Barreto; Silva,Marco Túlio Alves da; Garcia,José Fernando; Avelar,Gustavo Arbex; Porto Neto,Laercio Ribeiro; Marçon,André Ricardo; Bacci Junior,Maurício; Marini,Danyelle Cristine.
The main goal of our research was to search for SSRs in the Eucalyptus EST FORESTs database (using a software for mining SSR-motifs). With this objective, we created a database for cataloging Eucalyptus EST-derived SSRs, and developed a bioinformatics tool, named Satellyptus, for finding and analyzing microsatellites in the Eucalyptus EST database. The search for microsatellites in the FORESTs database containing 71,115 Eucalyptus EST sequences (52.09 Mb) revealed 20,530 SSRs in 15,621 ESTs. The SSR abundance detected on the Eucalyptus ESTs database (29% or one microsatellite every four sequences) is considered very high for plants. Amongst the categories of SSR motifs, the dimeric (37%) and trimeric ones (33%) predominated. The AG/CT motif was the most...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioinformatics tool; Genetic markers; Gene ontology; Small sequence repeats; SSR.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400014
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Genetic variability in the Skyros pony and its relationship with other Greek and foreign horse breeds Genet. Mol. Biol.
Bömcke,Elisabeth; Gengler,Nicolas; Cothran,E. Gus.
In Greece, seven native horse breeds have been identified so far. Among these, the Skyros pony is outstanding through having a distinct phenotype. In the present study, the aim was to assess genetic diversity in this breed, by using different types of genetic loci and available genealogical information. Its relationships with the other Greek, as well as foreign, domestic breeds were also investigated. Through microsatellite and pedigree analysis it appeared that the Skyros presented a similar level of genetic diversity to the other European breeds. Nevertheless, comparisons between DNA-based and pedigree-based results revealed that a loss of genetic diversity had probably already occurred before the beginning of breed registration. Tests indicated the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic variation; Genetic markers; Pedigree; Horse.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100013
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Association of CAPN1 and CAST gene polymorphisms with meat tenderness in Bos taurus beef cattle from Argentina Genet. Mol. Biol.
Corva,Pablo; Soria,Liliana; Schor,Alejandro; Villarreal,Edgardo; Cenci,Macarena Pérez; Motter,Mariana; Mezzadra,Carlos; Melucci,Lilia; Miquel,Cristina; Paván,Enrique; Depetris,Gustavo; Santini,Francisco; Naón,Juan Grigera.
The activity of the calpains/calpastatin proteolytic system is closely related to the postmortem tenderization of meat. We investigated the association between beef tenderness and single nucleotide polymorphism (SNP) markers on the CAPN1 gene (SNP316, alleles C/G; SNP530 alleles A/G) and the CAST gene 3' untranslated region (SNP2870, alleles A/G). We sampled nine slaughter groups comprising 313 steers which had been reared in beef production systems in Argentina between 2002 and 2004 from crosses between Angus, Hereford and Limousin cattle. Minor allele frequencies for the markers were 0.27 to 0.46 (C), 0.02 to 0.18 (A), and 0.24 to 0.53 (A), respectively. The presence of CAPN1 markers had significant effects on meat shear force but no detectable effects...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Beef cattle; CAPN1; CAST; Genetic markers; Meat quality.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600006
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Population variability in some genes involving the haemostatic system: data on the general population of Corsica (France), Sardinia and Sicily (Italy) Genet. Mol. Biol.
Falchi,Alessandra; Vacca,Lucia; Lopez Alomar,Antonio; Esteban,Esther; Memmi,Marc; Varesi,Laurent; Moral,Pedro; Vona,Giuseppe.
Three different population samples from Corsica (France), Sardinia and Sicily (Italy) were studied using nine genetic markers. For the first time, allele distributions of FGA TaqI, FGB Bcl I, FGB Hind III, PAI-1 Hind III, PLAT TPA-25, GPIIIa Taq I, GPIIb I/D 9bp, FVII HVR4 and FVII -323 10 bp markers, which are thought to be associated with cardiovascular disease risk, were studied in the general population of the three islands. The frequencies of the markers analysed in the present work show some peculiarities: the locus FVII HVR4 is characterized by the presence of a rare allele (H5), found in Corsicans and in Sardinians; the locus FBG BcII shows a low frequency of the B1 allele and the absence of the B1B1 genotype. The frequencies of some alleles have a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Haemostatic genes; Genetic markers; Mediterranean populations.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200002
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Implementation of a parentage control system in Portuguese beef-cattle with a panel of microsatellite markers Genet. Mol. Biol.
Carolino,Inês; Sousa,Conceição O.; Ferreira,Sónia; Carolino,Nuno; Silva,Fátima S.; Gama,Luís T..
A study was conducted to assess the feasibility of applying a panel of 10 microsatellite markers in parentage control of beef cattle in Portugal. In the first stage, DNA samples were collected from 475 randomly selected animals of the Charolais, Limousin and Preta breeds. Across breeds and genetic markers, means for average number of alleles, effective number of alleles, expected heterozygosity and polymorphic information content, were 8.20, 4.43, 0.733 and 0.70, respectively. Enlightenment from the various markers differed among breeds, but the set of 10 markers resulted in a combined probability above 0.9995 in the ability to exclude a random putative parent. The marker-set thus developed was later used for parentage control in a group of 140 calves from...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cattle; Genetic markers; Microsatellites; Parentage control.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200017
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Microsatellite genotyping from faeces of Lontra longicaudis from southern Brazil Iheringia, Sér. Zool.
Weber,Laura I.; Hildebrand,Cintia G.; Ferreira,Anderson; Pedarassi,Gustavo; Levy,José A.; Colares,Elton P..
A genetic study of the neotropical river otter Lontra longicaudis (Olfers, 1818), which has an unknown conservation status, was carried out at the Taim Ecological Station and the margins of the Vargas stream, Rio Grande do Sul, southern Brazil. Faecal samples were collected, and DNA was extracted using a silica-guanidine method. Five microsatellite loci were amplified using PCR with heterologous primers previously described for Lutra lutra (Linnaeus, 1758). Sixteen faecal samples out of 29 from Taim and 11 out of 14 from Vargas stream margins contained enough DNA for genetic analysis. A total of 49 different alleles were found at both localities, from which 18 were exclusively found in individuals from Taim and 17 were exclusives from Vargas individuals....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Neotropical river otter; Genetic markers; Taim; Non-invasive sampling; Genetic diversity.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0073-47212009000100001
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Extração de DNA e obtenção de marcadores moleculares para diferentes espécies de interesse para o cerrado. Infoteca-e
BELLON, G.; FALEIRO, F. G.; BARROS, A. M.; KARIA, C. T.; ANDRADE, R. P.; CORDEIRO, M. C. R.; PINTO, A. C.; JUNQUEIRA, N. T. V.; PEREIRA, A. V.; PEREIRA, E. B. C.; FERNANDES, F. D.; FERREIRA, M. E..
Tipo: Folders Palavras-chave: Planta nativa; Indigenous organisms; Biotecnologia; Biologia Molecular; Cerrado; DNA; Marcador Molecular; Marcador Genético; Genetic markers; Biotechnology; Molecular biology.
Ano: 2005 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/553057
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Análise de mistura genética em campos de multiplicação de sementes de soja com base em marcadores moleculares. Infoteca-e
FALEIRO, F. G.; SOUZA, P. I. M.; ANDRADE, S. R. M.; BELLON, G.; ABUD, S.; MOREIRA, C. T.; BARBOSA, F.; SANTOS, J. B..
Tipo: Folders Palavras-chave: Glycine Max; Marcador Molecular; Melhoramento Genético Vegetal; Soja; Genetic markers; Plant breeding; Soybeans.
Ano: 2005 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/566836
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Fingerprinting analysis of mango (Mangifera indica L.) cultivars introduced or developed in Brazil using RAPD markers. Infoteca-e
FALEIRO, F. G.; CORDEIRO, M. C. R.; PINTO, A. C. Q.; ROSSETTO, C. J.; BELLON, G.; ANDRSDE, S. R. M.; FRAGA, L. M. S.; SOUZA, T. L. P. O..
Tipo: Folders Palavras-chave: RAPD; Melhoramento genetico; Brasil; Mango; Biotecnologia; Marcador Genético; Marcador Molecular; Manga; Mangifera Indica; Brazil; Genetic markers; Biotechnology; Plant breeding.
Ano: 2004 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/566520
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Variabilidade genética de matrizes de pequizeiro selecionadas para estudos na Embrapa Cerrados e na UPIS- Faculdades Integradas, com base nos marcadores RAPD. Infoteca-e
BELLON, G.; FALEIRO, F. G.; CAMARGO, J. T.; ANDRADE, S. R. M. de; PEREIRA, A. V.; PEREIRA, E. B. C..
Tipo: Folders Palavras-chave: RAPD; Indigenous organisms; Caryocar Brasiliense; Espécie Nativa; Marcador Molecular; Melhoramento Genético Vegetal; Pequi; Variação Genética; Genetic variation; Genetic markers; Plant breeding.
Ano: 2005 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/569475
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Variabilidade genética de acessos de Arachis pintoi avaliados em diferentes centros de pesquisa da Embrapa com base em marcadores RAPD. Infoteca-e
FALEIRO, F. G.; ANDRADE, R. P. de; KARIA, C. T.; VALENTIM, J. F.; ANDRADE, C. M. S. de; RAMOS, A. K. B.; BELLON, G.; PENALOZA, A. D. P. de S.; VALLS, J. F. M..
Tipo: Folders Palavras-chave: RAPD; Feed legumes; Leguminosa Forrageira; Marcador Molecular; Melhoramento Genético Vegetal; Variação Genética; Genetic variation; Genetic markers; Plant breeding.
Ano: 2005 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/555267
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Análise da variabilidade genética molecular de uma coleção de trabalho de guandu (Cajanus cajan) como apoio na seleção de acessos para testes agronômicos em sistemas de integração lavoura-pecuária e recuperação de pastagens degradadas. Infoteca-e
FALEIRO, F. G.; FERNANDES, F. D.; BARCELLOS, A. O.; ANDRADE, R. P.; BELLON, G.; JUNQUEIRA, K. P.; AMABILE, R. F.; MARTHA JÚNIOR, G. B.; VILELA, L.; RAMOS, A. K. B.; KARIA, C. T.; GODOY, R..
Tipo: Folders Palavras-chave: Feed legumes; Biotecnologia; Marcador Genético; Leguminosa Forrageira; Marcador Molecular; Variação Genética; Genetic variation; Genetic markers; Biotechnology.
Ano: 2006 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/570173
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Marcadores morfológicos em coqueiros (Cocos nucifera L.): coloracão do coleto. Infoteca-e
ARAGAO, W. M.; CAMARA, T. M.; RIBEIRO, F. E..
Os marcadores de coloracao do coleto da plantula do coqueiro sao utilizados na certificacao de cruzamentos, baseado na heranca genetica das cores amarela, verde e marrom do coqueiro. Sua adequacao na certificacao e questionada.
Tipo: Folhetos Palavras-chave: Marcadores genéticos; Mapas genéticos; Hereditariedade genética; Genetic maps; Cocos nucifera L; Colour; Fenotypes; Genetic inheritance; Crossbreeding; Coco; Cor; Cruzamento; Fenótipo; Hibridação; Melhoramento Genético Vegetal; Genetic markers; Hybridization; Plant breeding.
Ano: 1999 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/370884
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Genotyping by sequencing approach for autotetraploid Urochloa Ruziziensis genetic breeding. Infoteca-e
RESENDE, R. M. S.; MARTINS, F. B.; VILELA, M. de M.; MORAES, A. C. L.; FERREIRA, R. C. U.; DÉO, T. G.; MOREIRA, F. A.; PASTINA, M. M.; NODA, R. W.; SOUZA, A. P..
Although Brazil is the major producer and exporter of beef in the world, the rangelands are cultivated with varieties obtained by selection on germplasm and conventional breeding. Only lately genomic information started to be used in the tropical forage breeding programs mostly because of the genome complexity and costs. Urochloa ruziziensis is a sexual autotetraploid forage that has a significant role in integrated systems that are currently important in the agribusiness in certain regions of Brazil and is essential as female parent in other Urochloa spp. breeding programs. Thus, this work intends to use the Genotyping by Sequencing (GBS) approach to identify SNP markers to construct the first map of the species and estimate breeding values for Genomic...
Tipo: Folders Palavras-chave: Genomics; Genetic markers; Pipelines; Breeding.
Ano: 2019 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1120361
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Operacionalizacao da extracao de DNA de especies nativas do cerrado visando analises moleculares. Infoteca-e
FALEIRO, F. G.; FALEIRO, A. S. G.; CORDEIRO, M. C. R.; KARIA, C. T..
Tipo: Folders Palavras-chave: Planta nativa; Indigenous organisms; Biologia Molecular; Biotecnologia; Cerrado; DNA; Marcador Genético; Marcador Molecular; Biotechnology; Genetic markers; Molecular biology.
Ano: 2004 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/567935
Registros recuperados: 73
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