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Registros recuperados: 26
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Eggplant (Solanum melongena L.): tissue culture, genetic transformation and use as an alternative model plant Acta Botanica
Magioli,Claudia; Mansur,Elisabeth.
Eggplant is an agronomically important non-tuberous solanaceous crop grown primarily for its large oval fruit. In popular medicine, eggplant is indicated for the treatment of several diseases, including diabetes, arthritis, asthma and bronchitis. Eggplant is susceptible to a number of diseases and pests capable of causing serious crop losses. This problem has been addressed by hybridizing eggplant with wild resistant Solanum species, which present a wide genetic diversity and are source of useful agronomic traits. The application of in vitro methodologies to eggplant has resulted in considerable success. Eggplant tissues present a high morphogenetic potential that is useful for developmental studies as well as for establishing biotechnological approaches...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Solanum melongena L.; Biotechnology; Tissue culture; Genetic transformation; Model plant.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062005000100013
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Influence of antibiotics on embryogenic tissue and Agrobacterium tumefaciens suppression in soybean genetic transformation Bragantia
Wiebke,Beatriz; Ferreira,Fabricio; Pasquali,Giancarlo; Bodanese-Zanettini,Maria Helena; Droste,Annette.
The influence of different antibiotic treatments in soybean genetic transformation was evaluated. First, an assay was performed to verify how different antibiotic treatments affect soybean embryogenic tissues. The effect of carbenicillin at 500 mg L-1 was genotype-dependent. This antibiotic did not affect embryo survival of cv. IAS5, but a three-fold increase of embryo proliferation was observed for cv. Bragg, when compared to the control. On the other hand, cefotaxime at 350 and 500 mg L-1 caused death of embryogenic tissues of both cultivars. Finally, the association of cefotaxime (250 mg L-1) + vancomycin (250 mg L-1) did not affect negatively the somatic embryos of tested cultivars until 63 days of treatment. Thereafter, a second experiment was carried...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agrobacterium tumefaciens; Carbenicillin; Cefotaxime; Genetic transformation; Glycine max; Vancomycin.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052006000400002
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Production of herbicide-resistant coffee plants (Coffea canephora P.) via Agrobacterium tumefaciens-mediated transformation BABT
Ribas,Alessandra Ferreira; Kobayashi,Adilson Kenji; Pereira,Luiz Filipe Protasio; Vieira,Luiz Gonzaga Esteves.
Transgenic plants of Coffea canephora P. resistant to the herbicide ammonium glufosinate were regenerated from leaf explants after co-culture with Agrobacterium tumefaciens strain EHA105 harboring pCambia3301, a plasmid that contains the bar and the uidA genes both under control of 35S promoter. Direct somatic embryogenesis was induced on basal medium contained ¼ strength macro salts and half strength micro salts of MS medium, organic constituents of B5 medium and 30 g.L-1 sucrose supplemented with 5µM N6 - (2-isopentenyl)-adenine (2-iP). Ten µM ammonium glufosinate was used for putative transgenic somatic embryos selection. Presence and integration of the bar gene were confirmed by PCR and Southern blot analysis. Selected transgenic coffee plants sprayed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic transformation; Coffee; Herbicide tolerance; Ammonium glufosinate.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100002
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Plant regeneration and Agrobacterium-mediated transformation of Achyranthes bidentata using cotton EREBP gene BABT
Duan,Hong Ying; Ding,Xiao Sheng; Song,Jian Ying; He,Yun Long; Zhou,Yan Qing.
The aim of this work was to study the plant regeneration and Agrobacterium-mediated transformation of Achyranthes bidentata using cotton EREBP gene. Results showed that the callus induction rate of stems from A. bidentata was the highest (100%) and bud was in approximately 70% of calli from stems. However bud differentiation rate of the callus from leaves and petioles was very low. Compared with ceftriaxone, 200mg/L cefotaxime could completely control Agrobacterium tumefaciens and had relatively less toxic action on the stems of A. bidentata. In addition, the induction rate of callus resistant to hygromycin was the highest when infected for 3 min and co-cultivated for 3 d. Six positive transgenic plants transformed with pCAMBIA1304-GhEREB2 expression...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Achyranthes bidentata; EREBP gene; Agrobacterium tumefaciens; Genetic transformation.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000300001
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Coffee biotechnology Braz. J. Plant Physiol.
De Los Santos-Briones,César; Hernández-Sotomayor,S. M. Teresa.
In the last three decades, interest has turned to in vitro cell culture in different areas of coffee research. In vitro techniques have been applied not only for coffee improvement through genetic transformation but also to study various aspects in coffee cells such as chemical (caffeine synthesis and the production of coffee aroma), physiological and more recently, biochemical aspects. The most important advances obtained to date on in vitro coffee techniques in fields like biochemistry, physiology, regeneration systems and genetic engineering, are presented and discussed.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coffea; Coffee; Genetic transformation; In vitro tissue culture; Regeneration; Somatic embryogenesis.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000100015
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Changes in peroxidase and polypeptide profiles in Nicotiana tabacum L. transformed with Agrobacterium rhizogenes Ciência Rural
Echeverrigaray,Sergio.
Morphological and proteic modifications in Nicotiana tabacum L. transformed by Agrobacterium rhizogenes were evaluated by the comparison of normal and transformant plants regenerated from hairy-roots formed by the strains A4 or IB-642 of A. rhizogenes. Changes in apical dominance were observed in IB-642 transformants, which exhibited an abnormal development of axiliary buds. The electrophoretic analysis indicated an increase in peroxidase activity and the induction of several isozymes of this complex in the transformants. The SDS-PAGE patterns comparison allows to identify several changes, specially, the increase in 31-33 and 54 kD polypeptides in the transformants.. Biochemical analysis suggests the induction of a pathogen or stress like response of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic transformation; Agrobacterium rhizogenes; Nicotiana tabacum; Electrophoretic analysis.
Ano: 1995 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781995000200009
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Plant regeneration via indirect somatic embryogenesis and optimisation of genetic transformation in Coffea arabica L. cvs. Caturra and Catuaí Electron. J. Biotechnol.
Gatica-Arias,Andrés M; Arrieta-Espinoza,Griselda; Espinoza Esquivel,Ana M.
A protocol for Coffea arabica L. cvs. Caturra and Catuaí plant regeneration via indirect somatic embryogenesis (ISE) was established. Furthermore, a biolistic mediated genetic transformation protocol was optimized for Catuaí callus aggregates. Maximum callus induction was obtained when Caturra (87%) and Catuaí (67%) leaves were cultured on Murashige and Skoog medium with 18.56 µM kinetin and 4.52 µM2,4-dichlorophenoxyacetic acid (2,4-D). Catuaí suspension cultures were established from embryogenic callus using liquid proliferation CP and Sli media and diffused light and darkness. The higher suspension cultures fresh weight was obtained using Erlenmeyer (1425.4 ± 354.9 mg) than Recipient for Automated Temporary Immersion System (RITA®) (518.6 ± 55.1 mg),...
Tipo: Journal article Palavras-chave: Bioreactor; Coffee; Genetic transformation; Somatic embryogenesis; Suspension cultures.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000100010
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Rooting in Km selective media as efficient in vitro selection method for sunflower genetic transformation Electron. J. Biotechnol.
Radonic,Laura Mabel; Zimmermann,Julián Marcos; Zavallo,Diego; López,Nilda; López Bilbao,Marisa.
Despite of numerous publications in sunflower genetic transformation, there is no efficient or reproducible protocol with low number of escapes. The latter would indicate that the selection method is not effective. In this work we used Km as selective agent, Agrobacterium tumefaciens EHA105 strain and a vector with the nptII gene under the nos promoter and uidA gene under 35S promoter. The response of agroinfected (A) and control (C) explants during the in vitro culture was studied and in both cases in presence or absence of Km in order to assign a differential morphologic response between transformed and non-transformed plants. The characteristics analyzed were: height, colour/aspect of the plantlets, in vitro rooting and in vitro bud-flower development....
Tipo: Journal article Palavras-chave: Genetic transformation; Kanamycin; Selection; Sunflower.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300027
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Efficient Agrobacterium tumefaciens-mediated transformation of Malus zumi (Matsumura) Rehd using leaf explant regeneration system Electron. J. Biotechnol.
Xu,Jin; Wang,Yu Zhen; Xia Yin,Heng; Jing Liu,Xiao.
Malus zumi is known as an excellent dwarfing apple rootstock occurring in natural or arid/semiarid soil or salina. Gene manipulation of M. zumi through transgenic technology can modify plant feature for further improvement fruit tree production by grafting the scion on a transgenic rootstock. Here, we report the establishment of an efficient, in vitro, shoot regeneration system and Agrobacterium tumefaciens- mediated transformation from the leaf explants for Malus zumi (Matsumura) Rehd. Leaf explants were infected with Agrobacterium strains containing nptII and gus gene. The highest frequency of shoot regeneration was obtained on MS medium containing 500 mg l-1 Lactalbumin hydrolysate, 30 g l-1 fructose, supplemented with 3.0 mg l-1 BA, 0.2 mg l-1...
Tipo: Journal article Palavras-chave: Agrobacterium tumefaciens; Apple rootstock; Genetic transformation.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000100003
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pGVG: a new Gateway-compatible vector for transformation of sugarcane and other monocot crops Genet. Mol. Biol.
Guidelli,Giovanna V.; Mattiello,Lucia; Gallinari,Rafael H.; Lucca,Paulo Cezar de; Menossi,Marcelo.
Abstract The successful development of genetically engineered monocots using Agrobacterium-mediated transformation has created an increasing demand for compatible vectors. We have developed a new expression vector, pGVG, for efficient transformation and expression of different constructs for gene overexpression and silencing in sugarcane. The pCAMBIA2300 binary vector was modified by adding Gateway recombination sites for fast gene transfer between vectors and the maize polyubiquitin promoter Ubi-1 (ZmUbi1), which is known to drive high gene expression levels in monocots. Transformation efficiency using the pGVG vector reached up to 14 transgenic events per gram of transformed callus. Transgenic plants expressing the β-glucuronidase (GUS) reporter gene...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Monocots; Sugarcane; Vector; Gateway technology; Genetic transformation.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300450
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Transformação de feijoeiro com gene Lhcb1*2 por meio do método SAAT ('Sonication-assited-agrobacterium transformation'). Infoteca-e
ANDRADE, S. R. M. de; GOMES, D. G.; LABATE, M. T. V.; LABATE, C. A..
Tipo: Folders Palavras-chave: Transformacao genetica; Transgenico; Transgenics; Biotecnologia; Feijão; Gene; Melhoramento Genético Vegetal; Phaseolus Vulgaris; Beans; Biotechnology; Genetic transformation; Plant breeding.
Ano: 2002 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/567647
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Transformacao de Phaseolus vulgaris Via SAAT ("Sonication-assisted agrobacterium- mediated transformation"). Infoteca-e
ANDRADE, S. R. M. de; GOMES, D. G.; LABATE, M. T. V.; LABATE, C. A..
Tipo: Folders Palavras-chave: Transgenico; Transformacao genetica; Transgenics; Biotecnologia; Feijão; Gene; Melhoramento Genético Vegetal; Phaseolus Vulgaris; Biotechnology; Genetic transformation; Beans; Genetics; Plant breeding.
Ano: 2002 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/567648
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Stability analysis of chickpea large genomic DNA inserts in Agrobacterium Open Agri
Rajesh, P.N..
Palavras-chave: Cloning; Genomes; Plasmids; DNA; Chickpeas; Genetic transformation; Chromosomes; Antibiotics; Bacteria; Genes.
Ano: 2007 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3293
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Biological activity of lectins from grain legumes and garlic against the legume pod borer, Helicoverpa armigera Open Agri
Arora, R.,( Richa Arora ).
Palavras-chave: Garlic; Pigeonpeas; Proteins; Transgenics; Transgenic plants; Chickpeas; Genes; Enzymes; Animal developmental stages; Genetic transformation.
Ano: 2005 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3230
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Germinating the seeds of success in the semi-arid tropics Open Agri
William D..
Palavras-chave: Pigeonpeas; Sorghum; Millets; Chickpeas; Transgenics; Biotechnology; Electrophoresis; Groundnuts; Agroecosystems; Genetic transformation.
Ano: 2006 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4145
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Reaction of sweet orange cultivars expressing the attacin A gene to 'Candidatus Liberibacter asiaticus' infection PAB
Felipe,Rafaella Teles Arantes; Mourão Filho,Francisco de Assis Alves; Lopes,Silvio Aparecido; Mendes,Beatriz Madalena Januzzi; Behling,Maurel; Pereira Junior,Ernani Vicente.
The objective of this work was to evaluate the reaction of four sweet orange cultivars expressing the attacin A gene to 'Candidatus Liberibacter asiaticus' (Las) infection, a bacterium associated to huanglongbing (HLB) disease. Transgenic sweet orange plants of Hamlin, Natal, Pêra, and Valência cultivars, as well as nontransgenic controls received inocula by grafting budwood sections of HLB-infected branches. Disease progression was evaluated through observations of leaf symptoms and by polymerase chain reaction (PCR) analysis, eight months after inoculation. A completely randomized design was used, with four experiments (one for each cultivar) performed simultaneously. Bacteria title was estimated by quantitative PCR (qPCR). HLB symptoms and Las titers...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus sinensis; Antibacterial peptide; Disease resistance; Genetic transformation; Huanglongbing.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013001100004
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Soybean cultivar BR-16-AHAS tolerance to the herbicide imazapyr PAB
Kiihl,Tammy Aparecida Manabe; Arias,Carlos Alberto Arrabal.
The objective of this work was to evaluate the effect of the transgenic soybean BR-16-AHAS genetic constitution on the tolerance to the herbicide imazapyr. BR-16-AHAS was crossed with ten other genotypes. The experimental design was a complete randomized block, in a 2x12x3 factorial arrangement, with two sowing periods (winter and summer), twelve crossing groups and three plant positions (upper, mid and lower), with three replicates. The plants were treated with 100 g ha-1 a.i. of imazapyr at the V3/V4 stage. For each position of the plant (upper, mid and lower), the following variables were assessed: number of pods, number of seeds, seed weight, number of seeds per pod and the 100 seeds weight. The effect of the herbicide varied according to the more...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arabidopsis thaliana; Glycine max; Epistasis; Genetic transformation.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000800012
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Isolation of a cotton NADP(H) oxidase homologue induced by drought stress PAB
NEPOMUCENO,ALEXANDRE LIMA; STEWART,JAMES MCD; OOSTERHUIS,DERRICK; TURLEY,RICKIE; NEUMAIER,MORMAN; FARIAS,JOSÉ RENATO BOUÇAS.
The aim of this study was to identify and isolate genes that are differentially expressed in four selected cotton (Gossypium hirsutum L.) genotypes contrasting according to their tolerance to water deficit. The genotypes studied were Siokra L-23, Stoneville 506, CS 50 and T-1521. Physiological, morphological and developmental changes that confer drought tolerance in plants must have a molecular genetic basis. To identify and isolate the genes, the mRNA Differential Display (DD) technique was used. Messenger RNAs differentially expressed during water deficit were identified, isolated, cloned and sequenced. The cloned transcript A12B15-5, a NADP(H) oxidase homologue, was up regulated only during the water deficit stress and only in Siokra L-23, a drought...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Messenger RNA; Gene expression; Genetic transformation; Water deficit.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2000000700015
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Transgenic tomato plants expressing the antigen gene PfCP-2.9 of Plasmodium falciparum PAB
Kantor,Mihail; Sestras,Radu; Chowdhury,Kamal.
The objective of this work was to obtain transgenic tomato plants expressing the PfCP-2.9 protein (a chimera of the antigens MSP1 and AMA1 of Plasmodium falciparum). Cotyledons of seven-day-old tomatoes, cultivar Summers, were transformed via Agrobacterium tumefaciens. Transgenic expression in the T0 plants was verified in the DNA extracted from fruits. PCR analysis was used to test the presence of the gene of interest in the T1 generation. Reverse transcriptase PCR provided evidence of gene expression at the RNA level, and Western blot analysis confirmed the presence of the protein of interest in the T1 plants. This is the first report of successful transformation with the expression of a malaria antigen (PfCP-2.9) in transgenic tomato plants from the T0...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gene expression; Genetic transformation; Malaria; Plant-made vaccines.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000100010
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Induction of transgenic hairy roots in soybean genotypes by Agrobacterium rhizogenes‑mediated transformation PAB
Weber,Ricardo Luís Mayer; Bodanese‑Zanettini,Maria Helena.
The objective of this work was to perform the screening of soybean genotypes as to their ability to respond to the induction of hairy roots by Agrobacterium rhizogenes‑mediated transformation. Four Brazilian soybean cultivars (BRSMG 68 Vencedora, BRS 137, Embrapa 48, and MG/BR 46 Conquista) and two North American ones adapted to Brazilian cropping conditions (Bragg and IAS‑5) were screened for their capacity to respond to A. rhizogenes in protocols for in vitro hairy root culture and ex vitro composite plant production. Four‑day‑old seedlings with uniform size were injected with A. rhizogenes harboring the plasmid p35S‑GFP. Seedlings expressing green fluorescent protein (GFP) in at least one hairy root were used to determine the transformation frequency....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max; Composite plants; Genetic transformation; Hairy roots culture; Strain K599.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000900014
Registros recuperados: 26
Primeira ... 12 ... Última
 

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