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Clostridium perfringens isolate typing by multiplex PCR J. Venom. Anim. Toxins incl. Trop. Dis.
Ahsani,MR; Mohammadabadi,MR; Shamsaddini,MB.
Clostridium perfringens is an important pathogen that provokes numerous different diseases. This bacterium is classified into five different types, each of which capable of causing a different disease. There are various methods for the bacterial identification, many are labor-intensive, time-consuming, expensive and also present low sensitivity and specificity. The aim of this research was to identify the different types of C. perfringens using PCR molecular method. In this study, 130 sheep-dung samples were randomly collected from areas around the city of Kerman, southeastern Iran. After processing and culturing of samples, the produced colonies were morphologically studied, gram stain test was also carried out and the genera of these bacteria were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Clostridium perfringens; Multiplex PCR; Biochemical tests; Genetic typing.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400006
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Carriage frequency, phenotypic and genotypic characteristics of Staphylococcus aureus isolated from dialysis and kidney tranplant patients at a hosptial in northern Paraná BJM
Giarola,Luciana Borges; Santos,Rosiane Ribeiro dos; Tognim,Maria Cristina Bronharo; Borelli,Sueli Donizete; Bedendo,João.
The objective of the present study was to determine the frequency of Staphylococcus aureus nasal carriage among dialysis and kidney transplant patients, to identify the antimicrobial resistance profile of these strains and to verify their genetic profiles with the RW3A primer. The study included 159 individuals, comprising 111 dialysis and 48 kidney transplant patients. Of the 48 transplant patients, 75% were positive for S. aureus, whereas 49% of the 111 dialysis patients were carriers. Two samples yielded conflicting results for oxacillin sensitivity between the disk diffusion and minimum inhibitory concentration (MIC) assays: both were sensitive by the disk diffusion assay and resistant by MIC (4 μg/ml). In the antibiogram by disk diffusion, ten samples...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus aureus; Nasal carrier; Resistance; MecA gene; Genetic typing.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300011
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