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| Islam,Mohammed M. |
| The N-terminal amino acid sequence of an aminopeptidase from Japanese edible mushroom, Grifola frondosa, was reported to have high similarity with that of a serine proteinase from basidiomycete, Agaricus bisporous (Nishiwaki and Hayashi, 2001). The full-length cDNA and the corresponding genomic DNA of the enzyme were cloned, based on the reported N-terminal amino acid sequence. The predicted open reading frame (ORF) of the cloned cDNA, encoding a product of 379 amino acids, was expressed in E. coli using pET expression vector. The expressed pro-enzyme (40 kDa) underwent autolysis to produce the mature protein (30 kDa) and a pro-peptide (10 kDa). The mature protein and the pro-peptide remained tightly bound to each other and could not be separated by Ni-NTA... |
| Tipo: Journal article |
Palavras-chave: 5'-RACE; CDNA; Genomic DNA; Maitake; Refolding. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000400010 |
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| Chapaval,L.; Moon,D.H.; Gomes,J.E.; Duarte,F.R.; Tsai,S.M.. |
| This study describes a rapid procedure for the isolation of genomic DNA from Staphylococcus aureus that yielded a good amount of high quality DNA for the amplification of staphylococcal enterotoxins genes (A, B, C, D, and E) and the TSST-1 gene as well as enzymatic restriction (HaeIII) from environmental isolates. With this method, it was possible to detect these genes in a sample containing as little as 10(5) cells with positive PCR reactions obtained from approximately 10pg of DNA in a final reaction volume of 25µl. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Staphylococcus aureus; Genomic DNA; DNA isolation. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000200004 |
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| Barra,Macarena; Salazar,Erika; Beltrán,María; Sagredo,Boris. |
| The extraction of high-quality DNA is essential for studies conducted at the molecular level in species with an abundance of contaminants in their tissues, such as some landraces of potato and maize, in which it is difficult to extract good-quality genomic DNA. Compounds such as polyphenols interfere with the amplification of DNA during polymerase chain reaction (PCR), which makes it difficult to conduct PCR-based studies ofmolecular markers. This article describes a simple and robust protocol for DNA isolation that was applied to potato and maize landraces and resulted in a high DNA concentration with excellent purity and quality. This method is based on the method of Krizman et al. (2006) with some modifications and was tested on potato leaves and young... |
| Tipo: Journal article |
Palavras-chave: Activated charcoal; DNA extraction; Genomic DNA; Polyphenols. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000300019 |
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| Nunes,Claudinéia Ferreira; Ferreira,Juliano Lino; Fernandes,Mônique Carolina Nunes; Breves,Sâmera de Souza; Generoso,Andressa Leal; Soares,Bárbara Dantas Fontes; Dias,Mário Sérgio Carvalho; Pasqual,Moacir; Borem,Aluízio; Cançado,Geraldo Magela de Almeida. |
| Several extraction methods of genomic DNA for identification and characterization of genetic diversity in different plant species are routinely applied during molecular analysis. However, the presence of undesirable compounds such as polyphenols and polysaccharides is one of the biggest problems faced during the isolation and purification of high quality DNA in plants. Therefore, achievement of fast and accurate methods for DNA extraction is crucial in order to produce pure samples. Leaves of strawberry genotypes (Fragaria ananassa) have high contents of polysaccharides and polyphenols which increase the sample viscosity and decrease the DNA quality, interfering with the PCR performance. Thereby, in this study we evaluated the quality and amount of genomic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fragaria ananassa; Genomic DNA; Polivinilpirrolidone; Lyophilization; PCR. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000800014 |
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