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Identification and characterisation of winter spawning ground in the English Channel and Southern North Sea ArchiMer
Lelievre, Stephanie.
A better knowledge and monitoring of principal commercial fish spawning grounds have become necessary in the North Sea. The efficiency of CUFES was proved by sampling pelagic fish eggs in winter in Eastern Channel and Southern North Sea. Fish egg taxonomic identification based on visual criteria cannot always be carried out effectively. In particular, cod (Gadus morhua), and whiting (Merlangius merlangus) or flounder (Platichthys flesus) and dab (Limanda limanda) have the same range of egg diameter and similar morphologies. Alternative identification methods using molecular techniques were developed to improve the accuracy of egg taxonomic identification. First, PCR-RFLP method, then, in order to accelerate egg identification, the use of a new laboratory...
Tipo: Text Palavras-chave: Oeufs de poissons; CUFES; VET; PCR-RFLP; Cyt b; 16S; ZooScan; Analyse d’images; Analyses géostatistiques; Interpolation; Modélisation d’habitat; GLM; RQ; Fish eggs; CUFES; VET; Image analysis; ZooScan; PCR-RFLP; Geostatistical analyses; Interpolated map; Habitat modelling; GLM; RQ.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00043/15398/12757.pdf
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PCR-RFLP analyses of formalin-fixed fish eggs for the mapping of spawning areas in the Eastern Channel and Southern North Sea ArchiMer
Lelievre, Stephanie; Verrez-bagnis, Veronique; Jerome, Marc; Vaz, Sandrine.
A better knowledge on spawning grounds of principal commercial fishes from North Sea is necessary for their monitoring. Fish egg taxonomic identification based on morphological characters cannot always be carried out effectively. This is particularly the case for a number of Gadidae fish species eggs (i.e. cod, haddock and whiting, and three rockling species) which have the same range of size and for which molecular-based techniques can help improving the accuracy of egg taxonomic identification, and hence the reliability of studies on spawning areas. In this study, formalin fixation, egg conservation and DNA extraction were optimized in order to develop a specific PCR-RFLP method. Based on the sequence of the cytochrome b gene of reference specimens, a...
Tipo: Text Palavras-chave: Fish eggs; PCR-RFLP; Cytochrome b gene; Geostatistical analyses; Interpolated map.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00005/11613/8259.pdf
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Integrating molecular identification of pelagic eggs with geostatistical mapping to improve the delineation of North Sea fish spawning grounds ArchiMer
Lelievre, Stephanie; Jerome, Marc; Maes, Gregory E.; Vaz, Sandrine; Calaivany, Sachidhanandam; Verrez-bagnis, Veronique.
Maps of the spawning grounds of commercially important fishes are necessary when assessing the level of connectivity between life stages of fishes and for identifying ecologically valuable marine areas. A first step toward mapping the spawning grounds is a reliable and rapid species identification of pelagic fish eggs to assess the spatio-temporal distribution of spawning aggregations. As many species have similar egg sizes and morphology, the molecular validation of visually identified eggs is often essential for the use of such data in fisheries management. In the present study, we developed a rapid 16S rRNA PCR-restriction fragment length polymorphism (RFLP) assay to distinguish between formalin-fixed fish eggs of dab Limanda limanda, flounder...
Tipo: Text Palavras-chave: Fish eggs; PCR-RFLP; 16S rRNA; Geostatistical analyses; Distribution; Spawning grounds.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00059/16999/14536.pdf
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