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Registros recuperados: 15 | |
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Faucet, Jerome; Maurice, Manuelle; Gagnaire, Beatrice; Renault, Tristan; Burgeot, Thierry. |
As the marine mussel Mytilus edulis is commonly used as a sentinel species, it would be useful to develop a primary culture of the target organs most often in contact with the marine environment. This study reports an improved method for dissociating the digestive gland and gills of M. edulis and considers the effect of mussel storage on cell viability and functionality before culture initiation. Viability and enzymatic activities such as those of esterase and peroxidase were monitored by flow cytometry, a sensitive, objective technique allowing large volumes of cells to be counted within a short time. A primary culture of digestive gland showed more than 75% viability after 72 h. Mussels were maintained in an aquarium containing clean, oxygenated seawater... |
Tipo: Text |
Palavras-chave: Mytilus edulis; Gill; Flow cytometry; Digestive gland; Cell culture. |
Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-936.pdf |
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Rivera-ingraham, Georgina A.; Rocchetta, Iara; Bickmeyer, Ulf; Meyer, Stefanie; Abele, Doris. |
Background: Reactive oxygen (ROS) and nitrogen (RNS) species are produced during normal unstressed metabolic activity in aerobic tissues. Most analytical work uses tissue homogenates, and lacks spatial information on the tissue specific sites of actual ROS formation. Live-imaging techniques (LIT) utilize target-specific fluorescent dyes to visualize biochemical processes at cellular level. Results: Together with oxidative stress measurements, here we report application of LIT to bivalve gills for ex-vivo analysis of gill physiology and mapping of ROS and RNS formation in the living tissue. Our results indicate that a) mitochondria located in the basal parts of the epithelial cells close to the blood vessels are hyperpolarized with high Delta Psi m, whereas... |
Tipo: Text |
Palavras-chave: Bivalve; Gill; Live-imaging; Fluorescence; Mitochondria; ROS; RNS. |
Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00626/73808/74412.pdf |
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Prieto, Daniel; Markaide, Pablo; Urrutxurtu, Iñaki; Navarro, Enrique; Artigaud, Sebastien; Fleury, Elodie; Ibarrola, Irrintzi; Urrutia, Miren Bego. |
The molecular basis underlying the mechanisms at the origin of growth variation in bivalves is still poorly understood, although several genes have been described as upregulated in fast-growing individuals. In the present study, we reared mussel spat of the species Mytilus galloprovincialis under diets below the pseudofaeces threshold (BP) and above the pseudofaeces threshold (AP). After 3 months, F and S mussels from each condition were selected to obtain 4 experimental groups: FBP, SBP, FAP and SAP. We hypothesized that the nurturing conditions during the growing period would modify the molecular basis of their growth rate differences. To decipher the molecular mechanisms underlying the growth variation, the gill transcriptomes for the four mussel groups... |
Tipo: Text |
Palavras-chave: Fast-growing; Mussel; Gill; Transcriptome. |
Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00503/61490/65293.pdf |
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Dubief, Bruno; Nunes, Flavia; Basuyaux, Olivier; Paillard, Christine. |
Since 1997, populations of the European abalone Haliotis tuberculata suffer mass mortalities attributed to the bacterium Vibrio harveyi. These mortalities occur at the spawning season, when the abalone immune system is depressed, and when temperatures exceed 17 °C, leading to favorable conditions for V. harveyi proliferation. In order to identify mechanisms of disease resistance, experimental successive infections were carried out on two geographically distinct populations: one that has suffered recurrent mortalities (Saint-Malo) and one that has not been impacted by the disease (Molène). Furthermore, abalone surviving these two successive bacterial challenges and uninfected abalone were used for several post-infection analyses. The Saint-Malo population... |
Tipo: Text |
Palavras-chave: Immunity; Hemocyte; Abalone; Disease; Extracellular products; Immune priming; Vibrio harveyi; Flow cytometry; Resistance; Phagocytosis; Bacterial growth; QPCR; Gill. |
Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00357/46800/46667.pdf |
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Alderman, D. J.; Gras, Paul. |
During April and May of this year, there has been a heavy mortality of Portuguese oysters, Crassostrea angulata LmK., which were imported into Britain from the River Tagus (Portugal) in March. Losses in some cases exceeded 90 per cent. The symptoms shown by the dyring oysters are the same as those seen in Britain and France in C. angulata in 1967 and 1968. Farley described the general symptoms in oysters (C. virginica Gmelin) dying of prolonged functional or infectious disease. These gross symtoms appear to be similar in the case of several different diseases including the present European mortality. |
Tipo: Text |
Palavras-chave: Gill; Crassostrea angulata; Oysters. |
Ano: 1969 |
URL: http://archimer.ifremer.fr/doc/1969/publication-5931.PDF |
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Ruiz-Picos,R; López-López,E. |
In aquatic ecosystems, the complex mixture of pollutants may mediate the formation of free radicals and cause oxidative damage to the biota. Yuriria Lake (a Ramsar site in Central Mexico) receives input of wastewater from its tributaries, agricultural runoff, and municipal discharge. We studied the lipid peroxidation (LPO), antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), and histopathology of gill and liver of the native fish Goodea atripinnis in Yuriria Lake. Results were compared to a control group of fish cultivated in the laboratory. LPO, SOD, and CAT showed no significant differences compared to controls, but GPx showed greater and significant differences in both tissues. Three class sizes were... |
Tipo: Journal article |
Palavras-chave: Mexican wetlands; Oxidative stress; Histopathology; Fish health condition; Liver; Gill. |
Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000300060 |
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Pereira,Bruno Fiorelini; Alves,Anderson Luis; Senhorini,José Algusto; Rocha,Rita de Cássia Gimenes de Alcântara; Pitol,Dimítrius Leonardo; Caetano,Flávio Henrique. |
Currently there is a growing concern, in both population and governments, to identify the effects of substances commonly disposed of into rivers and lakes, on aquatic fauna and flora. Thus the objective of the present study was to verify effects of biodegradable detergents and water from an urban lake on gills and liver of two neotropical fish species of great economic importance, Astyanax altiparanae and Prochilodus lineatus. In order to do so, lipofuscin, also called the ageing pigment, was used as bioindicator. After one and five months of experiment both tissues accumulated this pigment. These data are discussed from physiological points of view, related with lipid peroxidation and mitochondrial damage. |
Tipo: Journal article |
Palavras-chave: Lipofuscin; Liver; Gill; Prochilodus; Astyanax. |
Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000300005 |
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Spanghero,Diogo Bessa Neves; Spanghero,Emília Carolina Alencar de Medeiros; Pedron,Janaína dos Santos; Chagas,Edsandra Campos; Chaves,Francisco Célio Maia; Zaniboni-Filho,Evoy. |
Abstract: The objective of this work was to evaluate peppermint (Mentha piperita) essential oil toxicity to and potential as an anesthetic for juvenile silver catfish (Rhamdia quelen). To determine the lethal concentration at 4 hours (LC50-4h), 210 fish (3.08±0.8 g and 7.59±0.67 cm) were exposed to 0, 20, 50, 80, 110, and 140 mg L-1 essential oil. To evaluate the anesthetic potential, nine fish were individually exposed to each oil concentration (50, 80, 110, and 140 mg L-1) used. Water quality parameters were controlled. The mortality rate and the severity and extent of the gill injuries of silver catfish juveniles at 4 hours of exposure increased with increasing peppermint essential oil concentrations, with LC50-4h estimated to be 75.06 mg L-1. The main... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mentha piperita; Rhamdia quelen; Anesthesia; Gill; Histology; Lethal concentration. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2019000101102 |
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Machado,Marcelo Rubens; Fanta,Edith. |
Gills are vital structures for fish, since they are the main site for gaseous exchange as well as partially responsible for osmorregulation, acid-basic balance, excretion of nitrogenous compounds and taste. Chemicals in the water may alter the morphology of branchial cells of fish that are, therefore, a useful model for environmental impact and ecotoxicology studies. In order to investigate the effects of an organophosphorous compound, methyl parathion, on the gills of the fish, samples of Metynnis roosevelti were exposed to lethal (7ppm) and sublethal (1ppm) doses of Mentox 600 CE. Through light and scanning electron microscopy, shrinking of the branchial epithelium, followed by detachment and hyperplasia were observed. Externally, the branchial filaments... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gill; Fish; Methyl parathion; Toxicity; Morphology. |
Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000300008 |
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Fontaínhas-Fernandes,António; Luzio,Ana; Garcia-Santos,Sofia; Carrola,João; Monteiro,Sandra. |
Adult Nile tilapia, Oreochromis niloticus, of both sexes were exposed in wastewater from a sewage treatment plant for a period of 4 days. Gill samples were collected after 24, 48, 72 and 96 h and histopathological changes were analyzed by light and scanning electronic microscopy. Gill epithelium of control O. niloticus (freshwater group) was similar to that of other teleosts, while histopathological lesions were observed in exposed fishes. The main histopathological changes were edema, lifting of lamellar and filamentar epithelia and lamellar fusion. Cell proliferation with consequent thickening of the filament epithelium was also found in fishes exposed to the treated sewage water. The severity of the lesions increased with the time of exposure, namely... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gill; Tilapia; Histopathology; Wastewater. |
Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500023 |
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Registros recuperados: 15 | |
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