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Gluconeogenic enzyme gene expression is decreased by dietary carbohydrates in common carp (Cyprinus carpio) and gilthead seabream (Sparus aurata) ArchiMer
Panserat, Stéphane; Plagnes Juan, E; Kaushik, Sadasivam.
Our objective is to understand the low metabolic utilization of dietary carbohydrates in fish. We compared the regulation of gluconeogenic enzymes at a molecular level in two fish species, the common carp (Cyprinus carpio) and gilthead seabream (Sparus aurata), known to be relatively tolerant to dietary carbohydrates. After cloning of partial cDNA sequences for three key gluconeogenic enzymes (glucose-6-phosphatase (G6Pase), fructose biphosphatase (FBPase) and phosphoenolpyruvate carboxykinase (PEPCK) in the two species, we analyzed gene expressions of these enzymes 6 and 24 h after feeding with (20%) or without carbohydrates. Our data show that there is at least one gluconeogenic enzyme strongly regulated (decreased expression after feeding) in the two...
Tipo: Text Palavras-chave: Gilthead seabream; Common carp; Gluconeogenesis; Gene expression; Hepatic glucose metabolism; Dietary carbohydrate.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/2002/publication-668.pdf
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Influence of lupin and rapeseed meals on the integrity of digestive tract and organs in gilthead seabream (Sparus aurata L.) and goldfish (Carassius auratus L.) juveniles ArchiMer
Omnes, Marie-helene; Silva, Francisco; Moriceau, Jacques; Aguirre, P.; Kaushik, Sadasivam; Gatesoupe, F. J..
Two rearing trials were conducted to evaluate the effects of dietary lupin (LM) and rapeseed (RM) meals in gilthead seabream (Sparus aurata L.) and goldfish (Carassius auratus L.) juveniles. Each plant meal was incorporated at the rate of 200 g kg−1 in two distinct diets, which were compared with a fishmeal-based diet as control. After 1 month, the two plant diets did not influence the whole body growth, but the digestive systems were affected. The splenosomatic index was reduced with two plant meals in goldfish and with RM in seabream. The hepatosomatic index was only reduced in LM-fed seabream. Cellular characteristics were also affected. The largest liver cells were observed in RM-fed goldfish suggesting changes in metabolic function. The LM and RM...
Tipo: Text Palavras-chave: Digestive tract; Gilthead seabream; Goldfish; Histology; Lupin; Rapeseed.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00190/30107/28742.pdf
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Effets de la congélation sur le profil protéique des filets de la Dorade royale (Sparus aurata): comparaison entre le poisson sauvage et d’élevage OceanDocs
Attouchi, M.; Sadok, S..
Les variations des profils éléctrophorétiques des protéines sarcoplasmiques (SPP) et myofibrillaires (MFP) chez les filets de la Dorade royale d’origine sauvage et d’élevage congelés à -18°C ont été suivies durant 4 mois de stockage. A l’état frais, la différence de l’origine de production de la Dorade (sauvage/aquaculture) n’a pas illustré de distinctions particulières dans les profils protéiques des filets. Durant le stockage congelé, l’analyse densitométrique des profils MFP et SPP chez les deux populations de Dorade a permis de discerner des modifications mineures liées à l’origine de production du poisson et à la progression du temps de stockage. Ces faibles changements, notamment dans la fraction MFP, ont été plus précoces chez la Dorade d’élevage....
Tipo: Journal Contribution Palavras-chave: Seabream fisheries; Gilthead seabream.
Ano: 2011 URL: http://hdl.handle.net/1834/4744
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