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| Shukla,Shraddha; Goyal,Arun. |
| A glucan producing Weissella confusa isolated from fermented cabbage, as identified earlier, was used for optimization of its fermentation medium. The effects of various macronutrients such as sucrose, glucose as cosubstrate, yeast extract, beef extract, peptone, sodium acetate, K2HPO4 and Tween 80 were studied on glucansucrase and glucan production from Weissella confusa. The medium used as control gave 6.0 U/ml enzyme activity and 34 mg/ml glucan concentration. Sucrose (5%), glucose as cosubstrate (5% for glucansucrase and 3% for glucan production), Tween 80 (0.1%), yeast extract (1.5%), Peptone (2.0%) and K2HPO4 (1.5%) were effective nutrients displaying higher glucansucrase and glucan production giving 18.2, 18.0, 7.0, 6.4, 6.2 and 6.4 U/ml enzyme... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Weissella confusa; Glucansucrase; Glucan; Tween 80. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600006 |
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| Picka,M. C. M.; Calvi,S. A.; Lima,C. R. G.; Santos,I. A. T.; Marcondes-Machado,J.. |
| Acute infection by Toxoplasma gondii leads to suppression of cell-mediated immunity, facilitating chronic infection. One of the causes of immunosuppression is Interleukin-10 (IL-10) production. Glucan is used to stimulate phagocytosis. Our objective was to study IL-10 induction in male BALB/c mice with acute T. gondii BTU-2 strain infection, glucan immunostimulation, and sulfadiazine treatment. Animals were distributed into 7 groups: G1: infected with T. gondii; G2: infected with T. gondii and treated with sulfadiazine; G3: infected with T. gondii and immunostimulated with glucan; G4: infected with T. gondii, immunostimulated with glucan, and treated with sulfadiazine; G5: imunostimulated with glucan; G6: treated with saline; and G7: treated with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Toxoplasma gondii; Interleukin-10; Glucan; Immune response; Sulfadiazine. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400012 |
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| Pereira,Pedro Antonio Arraes; Oliver,Ann; Bliss,Fredrick Allen; Crowe,Lois; Crowe,John. |
| The objective of this study was to determine if trehalose can effectively enhance the viability and storage lifetime of Rhizobium tropici and Rhizobium etli after lyophilization as compared to the traditional protective combination of sucrose and peptone. Two strains of bean-nodulating rhizobia were effectively preserved in the lyophilized state by 100 mM trehalose, and survived for at least 12 days, even when stored under non-ideal conditions. Trehalose provided better protection than the sucrose/peptone combination. When protective excipients were introduced inside the cells, strain CFN 42 was more sensitive to the type of additive used than strain CIAT 899, probably because CIAT 899 produces beta (1-2) glucan, which may have a protective effect. beta... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Storage; Inoculation; Symbiosis; Glucan. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2002000600012 |
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