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Registros recuperados: 9
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Evaluation of Antimicrobial Activity of Glucose Oxidase from Aspergillus niger EBL-A and Penicillium notatum BABT
Zia,Muhammad Anjum; Riaz,Ayesha; Rasul,Samreen; Abbas,Rao Zahid.
This work aimed to study the production and purification of glucose oxidase by Aspergillus niger and Penicillium notatum using corn steep liquor as the substrate and evaluate its antimicrobial activity for use in pharmaceutical and food industries. The enzyme was purified by ammonium sulfate precipitation (60-85%), DEAE-cellulose ion exchange and Sephadex G-200 size exclusion chromatography. The crude enzyme extracts of A. niger and P. notatum showed 2.32 and 5.53 U mg-1 specific activities, respectively, which after desalting was 15.52 and 12.05 U mg-1, and after ion exchange and gel filtration chromatography was 29.09 - 62 and 25.72 - 59.37 U mg-1 for A. niger and P. notatum, respectively. The antimicrobial activity was determined by disc diffusion...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glucose oxidase; Antimicrobial; Aspergillus niger; Penicillium notatum.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600010
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Production of rabbit antibodies against purified Glucose oxidase BABT
Zia,Muhammad Anjum; Ain,Qurat-ul; Iftikhar,Tehreema; Abbas,Rao Zahid; Rahman,Khalil-ur.
Glucose oxidase is an active oxygen species generating enzyme produced from Aspergillus niger grown in submerged fermentation. Disintegration of the mycelium resulted in high glucose oxidase activity that was subjected to ammonium sulfate precipitation at 60-85% saturation rates that resulted to 6.14 U mg -1 specific activity. Purification of enzyme by anion exchange column (DEAE-Cellulose) resulted into 22.53 U mg-1 specific activity and 10.27 fold purification. This was applied to sephadex G-200 column for gel filtration chromatography. It was observed that enzyme achieved 59.37 U mg-1of specific activity with 27.08 fold purity and 64.36% recovery. Purified glucose oxidase was injected into rabbits through intravenous route, to raise the glucose oxidase...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glucose oxidase; A. niger; Antibodies; Rabbits.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100008
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Partition of glucose oxidase from Aspergillus niger in aqueous two-phase systems based on salt and polyethylene glycol BABT
Singh,Jagdish; Verma,Neelam.
The aim of this work was to study the isolation of glucose oxidase (GOx) from Aspergillus niger in aqueous two phase system consisting of PEG 7500 (150g l-1), potassium phosphate (175 g l-1, K2HPO4 +KH2PO4) and glucose (10 gl-1), the enzyme was partitioned in polymer phase. By sequential extraction GOx (69.2%) was recovered in polymer phase by 11.8 fold purification, giving a yield of 129U mg protein-¹.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus niger; Glucose oxidase; Aqueous two phase.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500007
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Screening of filamentous fungi for production of enzymes of biotechnological interest BJM
Guimarães,Luis Henrique S.; Peixoto-Nogueira,Simone C.; Michelin,Michele; Rizzatti,Ana Carolina S.; Sandrim,Valéria C.; Zanoelo,Fabiana F; Aquino,Ana Carla M.M.; Junior,Altino B.; Polizeli,Maria de Lourdes T. M..
Many enzymes produced by fungi have relevant biotechnological applications in several industrial areas. The purpose of this study was to collect and isolate filamentous fungi from soil and humus, plants and sugar cane bagasse of different regions of the São Paulo state. Forty isolates were examined for their ability to produce xylanase, glucose-oxidase, alkaline phosphatase, acid phosphatase, phytase, pectinase and amylase. Among these, twenty three isolates exhibited enzymatic potential. The xylanases produced by two of these isolates (Aspergillus caespitosus and A. phoenicis) showed good potential for pulp bleaching. Among seventeen isolates, at least three produced high levels of glucose-oxidase, being Rhizopus stolonifer and A. versicolor the best...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phosphatase; Xylanase; Glucose oxidase; Pectinase; Amylase; Rhizopus microsporus var. rhizopodiformis; Fungi.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400014
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Invertase, glucose oxidase and catalase for converting sucrose to fructose and gluconic acid through batch and membrane-continuous reactors BJPS
Silva,Aline Ramos da; Tomotani,Ester Junko; Vitolo,Michele.
Conversion of sucrose into fructose and gluconic acid using invertase, glucose oxidase and catalase was studied by discontinuous (sequential or simultaneous addition of the enzymes) and continuous (simultaneous addition of the enzymes in a 100 kDa-ultrafiltration membrane reactor) processes. The following parameters were varied: concentration of enzymes, initial concentration of substrates (sucrose and glucose), pH, temperature and feeding rate (for continuous process). The highest yield of conversion (100%) was attained through the discontinuous (batch) process carried out at pH 4.5 and 37 ºC by the sequential addition of invertase (14.3 U), glucose oxidase (10,000 U) and catalase (59,000 U).
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sucrose/conversion; Fructose; Gluconic acid; Invertase; Glucose oxidase; Catalase.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200022
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Effect of glucose oxidase addition on the textural characteristics of wheat-maize dough and bread Ciênc. Tecnol. Aliment.
KOUASSI-KOFFI,Jean Didier; STURZA,Alina; PĂUCEAN,Adriana; MAN,Simona; MUREȘAN,Andruța Elena; PETRUȚ,Georgiana; MUREȘAN,Vlad; MUSTE,Sevastița.
Abstract The aim of this study was to assess the effect of adding glucose oxidase (GOX) on wheat-maize dough and bread textural characteristics. It strengthens the importance and need of replacing wheat flour with maize flour in regions where extensive cultivation of wheat is not possible. For improving the rheological properties of bread, GOX was added to wheat and wheat-maize flours. Wheat and maize flours were used in several ratios, but the best results were obtained for (on flour basis): 80:20, 75:25, 70:30, where the highest values correspond to the wheat flour. Wheat-maize dough and bread volume were slightly influenced by GOX addition. Dough strength was higher in the case of dough without GOX addition than the samples enriched by GOX, while crumb...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dough rheology; Bread freshness and hardening; Glucose oxidase; Maize flour.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019000100127
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Biosensor for determination of glucose in real samples of beverages Ciênc. Tecnol. Aliment.
Lopes,Flavio Marques; Batista,Karla de Aleluia; Batista,Gustavo Luiz Aleluia; Fernandes,Kátia Flávia.
A biosensor was developed for spectrophotometric determination of glucose concentrations in real samples of orange juice energetic drinks, and sport drinks. The biosensor consisted of glucose oxidase (GOD) and horseradish peroxidase (HRP) immobilized onto polyaniline activated with glutaraldehyde (PANIG). Immobilization parameters were optimized for GOD, and maximum immobilization yield was 16% when 5.0 mg of PANIG and 8.9 U prepared in 0.1 mol.L-1 sodium phosphate buffer (pH 7.0) reacted for 60 minutes at 4 °C with gentle stirring. The linear operational range for glucose determination using optimized operational parameters was between 0.05 and 6.0 mg.mL-1 with a very good reproducibility of response. The results obtained in the biosensor were compared...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biosensor; Glucose; Glucose oxidase; Immobilization; Polyaniline.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000100010
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Characteristics of glucose oxidase immobilized on Magnetic Chitosan Nanoparticles Ciênc. Tecnol. Aliment.
EIN ALI AFJEH,Maryam; POURAHMAD,Rezvan; AKBARI-ADERGANI,Behrouz; AZIN,Mehrdad.
Abstract Immobilization process is used to facilitate the enzyme recovery and reusability. The aims of this study were to evaluate the effect of different amounts of chitosan on immobilization efficiency of Glucose Oxidase (GOX), pH effect on enzyme activity and changes of kinetic characteristics of enzyme immobilized on Magnetic Chitosan Nanoparticles (MCNP). GOX was immobilized on MCNP with cross bonds and Schiff base covalent connections. The sample with 0.3 g of chitosan had the highest immobilization efficiency (41.30%). Morphology of nanoparticles was investigated by Scanning Electron Microscopy (SEM). Immobilization of GOX on magnetic nanoparticles was verified with Fourier Transform Infrared (FTIR). The effect of pH on enzyme activity was similar...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chitosan nanoparticles; Enzyme activity; Glucose oxidase; Immobilization efficiency; Kinetic parameters.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612020000100068
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Imobilização de glicose oxidase para a síntese de ácidos aldônicos. Infoteca-e
VAZ JUNIOR, S.; RODRIGUES, D. de S.; GONCALVES, S. B.; ALMEIDA, J. R. M. de; CARVALHO, W.; PACHECO, T. F..
Os ácidos aldônicos — glicônico e xilônico — estão entre as moléculas de alto valor agregado que podem ser obtidas a partir de biomassa lignocelulósica e apresentam elevado potencial de aplicação comercial em diferentes campos. A síntese de ácidos aldônicos é possível por meio de diferentes processos, como: químico, eletroquímico, microbiano e enzimático. Porém, independentemente da opção escolhida, ainda existem vários obstáculos para que a produção em larga escala seja bem estabelecida. Entre esses obstáculos, está o desenvolvimento de um catalisador eficaz e estável para a síntese dos ácidos aldônicos a partir de hidrolisados de biomassa lignocelulósica, cuja composição apresenta, além dos monômeros de interesse (glicose e xilose), um grande número de...
Tipo: Folhetos Palavras-chave: Biocatalisador; Ácidos aldônicos; Biocatálise; Reator de leito fixo; Ácido glicônico; Ácido xilônico; Glicose oxidase; Catalisador; Green chemistry; Biocatalysis; Biomass; Glucose oxidase.
Ano: 2020 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1124141
Registros recuperados: 9
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