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Genetic transformation studies and scale up of hairy root culture of Glycyrrhiza glabra in bioreactor Electron. J. Biotechnol.
Mehrotra,Shakti; Kumar Kukreja,Arun; Singh Khanuja,Suman Preet; Nath Mishra,Bhartendu.
The study was undertaken to induce hairy roots in Glycyrrhiza glabra in leaf explants and to optimize the nutritional requirement for its growth kinetics at shake flask and bioreactor level. Pathogenecity of Agrobacterium depends upon transformation ability of strain and age, type, and physiological state of explants. Agrobacterium rhizogenes strain K599 was used to infect leaf explants of G. glabra. Explants of different age groups were obtained from 2 to 5 weeks old in vitro grown cultures. Bacterial strain K599 could induce hairy roots in 3 and 4 weeks old leaf explants cultured on B5, MS, NB and WP basal semi-solid medium. Leaf explants of 2 and 5 weeks old culture were not responsive to bacterial infection in terms of hairy root induction. Maximum...
Tipo: Journal article Palavras-chave: Agrobacterium; Bioreactor; Glycyrrhiza; Scale-up; Transformed roots.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000200009
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