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Campana,Eloiza Helena; Xavier,Danilo Elias; Petrolini,Fernanda Villas-Boas; Cordeiro-Moura,Jhonatha Rodrigo; Araujo,Maria Rita Elmor de; Gales,Ana Cristina. |
Abstract The mechanisms involved in the uncommon resistance phenotype, carbapenem resistance and broad-spectrum cephalosporin susceptibility, were investigated in 25 Pseudomonas aeruginosa clinical isolates that exhibited this phenotype, which were recovered from three different hospitals located in São Paulo, Brazil. The antimicrobial susceptibility profile was determined by CLSI broth microdilution. β-lactamase-encoding genes were investigated by PCR followed by DNA sequencing. Carbapenem hydrolysis activity was investigated by spectrophotometer and MALDI-TOF assays. The mRNA transcription level of oprD was assessed by qRT-PCR and the outer membrane proteins profile was evaluated by SDS-PAGE. Genetic relationship among P. aeruginosa isolates was assessed... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gram-negative bacilli; Carbapenem resistance; OprD porin. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000100057 |
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Gupta,Varsha; Datta,Priya; Agnihotri,Nalini; Chander,Jagdish. |
We examined the drug susceptibility pattern of Gram-negative bacilli to seven new beta-lactams. A total of 277 non-duplicate gramnegative bacilli strains belonging to the Enterobacteriaceae family, Pseudomonas and Acinetobacter species, isolated from various clinical samples were tested for susceptibility to imipenem, piperacillin/tazobactam, cefoperazone/sulbactam, ticarcillin/clavulanate, cefdinir, cefepime and cefpirome with the disk diffusion technique. The percentage resistance was low for imipenem (7.2%), piperacillin/tazobactam (2.8%), cefoperazone/sulbactam (5.4%). However, a high frequency of resistance was observed to ticarcillin/clavulanate (83.9%), cefdinir (70.6%), cefepime (45.5%) and cefpirome (84.4%). We conclude that imipenem,... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Beta-lactam; Beta-lactam/beta-lactamase inhibitor; Gram-negative bacilli. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000100005 |
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