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Registros recuperados: 15 | |
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BUSTAMANTE,JAVIER ANDRÉS; ASTUDILLO,MIRYAM; PAZOS,ALVARO JAIRO; BRAVO,LUIS EDUARDO. |
Los tejidos de archivo son material de incalculable valor para estudios retrospectivos que requieran la aplicación de análisis moleculares. Existen múltiples métodos de extracción de ADN a partir de este tipo de muestras. No obstante, la mayoría de métodos toman mucho tiempo y los reactivos empleados contribuyen a la fragmentación del ADN. Con el objetivo de optimizar dos métodos de extracción de ADN a partir de tejidos embebidos en parafina en condiciones no óptimas, se seleccionaron 47 bloques en parafina que contenían biopsias de pleura, pulmón y pericardio correspondientes a 24 pacientes (66,6% hombres) mayores de 18 años, con inflamación granulomatosa crónica, remitidos al Departamento de Patología, Hospital Universitario del Valle entre 2002 y 2007.... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biopsia; Granuloma; Ácidos nucleicos; Fragmentación del ADN; Reacción en cadena de la polimerasa. |
Ano: 2011 |
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2011000200007 |
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BUSTAMANTE,JAVIER ANDRÉS; ASTUDILLO,MIRYAM; PAZOS,ALVARO JAIRO; LUIS EDUARDO,BRAVO. |
La detección de bacilos ácido alcohol resistentes (BAAR) en frotis resulta un aporte significativo al diagnóstico y tratamiento de individuos con inflamación granulomatosa crónica (IGC). Con el objetivo de evaluar la presencia de BAAR en diferentes tejidos de pa-cientes con IGC demostrada y comparar la frecuencia de los extendidos positivos para BAAR con el grado de formación de granulomas y necrosis, se diseño un estudio retrospectivo, en el que se seleccionaron 57 bloques embebidos en parafina que contenían biopsias de pleura, pulmón, pericardio, ganglio linfático y lóbulo cerebral, correspon-dientes a 30 pacientes (63,3% hombres), mayores de 18 años, con IGC demostrada y remitidos al departamento de patología del Hospital Universitario del Valle entre... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biopsia; Granuloma; Necrosis; Infecciones por mycobacterium; Diagnóstico. |
Ano: 2010 |
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2010000200018 |
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Tristão,A.R.; Melo,A.L.; Vasconcelos,A.C.; Grossi,F.M.. |
Foram estudadas 42 amostras de fígado de camundongos inoculados com cercárias do Schistosoma mansoni, obtidas 40, 60, 80 e 120 dias após a infecção e processadas rotineiramente. As lâminas obtidas foram coradas pela HE para análise qualitativa e morfométrica do número e área dos granulomas e pelo MGP para quantificação de células apoptóticas. Os animais com 40 dias de inoculação possuíam menos granulomas/lâmina (<img src="http:/img/fbpe/abmvz/v52n6/a06img01.gif" alt="a06img01.gif (532 bytes)" align="absmiddle" > ou = 11,78±4,01), com áreas pequenas (<img src="http:/img/fbpe/abmvz/v52n6/a06img01.gif" alt="a06img01.gif (532 bytes)" align="absmiddle" > ou = 52.713,88±5.244,34<FONT FACE="Symbol">m</font>m²) e as menores médias de... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Schistosoma mansoni; Apoptose; Morte celular programada; Modulação da inflamação; Granuloma. |
Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000600006 |
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Lenzi,H.L.; Kimmel,E.; Schechtman,H.; Pelajo-Machado,M.; Vale,B.S.; Panasco,M.S.; Lenzi,J.A.. |
The collagen structure of isolated and in situ liver granuloma from Swiss Webster mice infected with Schistosoma mansoni was sequentially and three-dimensionally analyzed during different times of infection (early acute, acute, transitional acute-chronic, and chronic phases) by laser scanning confocal microscopy and electron scanning variable vacuum microscopy. The initial granuloma structure is characterized by vascular collagen residues and by anchorage points (or fiber radiation centers), from where collagenous fibers are angularly shed and self-assembled. During the exudative-productive stage, the self-assembly of these fibers minimizes energy and mass through continuous tension and focal compression. The curvature or angles between collagen fibers... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Granuloma; Schistosoma mansoni; Collagen; Extracellular matrix; Confocal microscopy. |
Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500018 |
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Conte,F.P.; Fidalgo-Neto,A.A.; Manhães-Rocha,D.A.; Paumgartten,F.J.R.; De-Oliveira,A.C.A.X.. |
The effects of schistosomiasis on microsomal enzymes were studied on post-infection day 90 when accumulated damage and fibrosis are most intense but granulomatous reaction around the eggs harbored in the liver is smaller than during the earlier phases. Swiss Webster (SW) and DBA/2 mice of either sex (N = 12 per sex per group) were infected with 100 Schistosoma mansoni cercariae on postnatal day 10 and killed on post-infection day 90. Cytochrome P-450 (CYP) concentration and alkoxyresorufin-O-dealkylases (EROD, MROD, BROD, and PROD), p-nitrophenol-hydroxylase (PNPH), coumarin-7-hydroxylase (COH), and UDP-glucuronosyltransferase (UGT) activities were measured in hepatic microsomes. Age-matched mice of the same sex and strain were used as controls. In S.... |
Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Schistosoma mansoni; Pharmacokinetics; Cytochrome P-450; Monooxygenases; Granuloma; Liver microsomes. |
Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500008 |
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Curcio,Bruna da Rosa; Gomes,Fabiane Resende; Melo,Dário Milanez; Raffi,Margarida Buss; Riet-Correa,Franklin; Ladeira,Sílvia Regina Leal. |
Foi observada em um bovino com suspeita de lechiguana, uma lesão arredondada de consistência dura na região sub-mandibular que media 12x11x7cm. Foram realizadas biópsias cirúrgicas para estudos bacteriológico e histológico, teste de tuberculinização intra-dérmico e coleta de sangue, a fim de realizar o teste de imunodifusão radial para o diagnóstico de lechiguana. Posteriormente, foi realizada eutanásia e necropsia do animal. No resultado das biópsias, não se obteve isolamento bacteriano e o estudo histológico não foi conclusivo. Os testes de tuberculinização e de imunodifusão apresentaram resultado negativo. Na necropsia, constatou-se que a lesão consistia de um nódulo, formado por uma cápsula de tecido conjuntivo envolvendo uma massa esbranquiçada com... |
Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Granuloma actinomicóide; Granuloma; Arcanobacterium pyogenes; Bovinos. |
Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782002000500023 |
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D’Elia,Mirella Lauria; Santos,Alice Barroso; Ribeiro,Beatriz Novaes Telles; Torres,Renato Cesar Sacchetto; Santos,Renato de Lima; Silva,Rodrigo Otávio Silveira; Nepomuceno,Anelise Carvalho. |
ABSTRACT: A 6-year-old Fuzzy Lop rabbit was referred to a veterinary hospital with a complaint of lameness. In addition to a vertebral subluxation, two radiopaque and well-defined structures were revealed by radiographic evaluation. Ultrasonographically, the masses were characterized as parenchymal structures with diffuse mineralization and formation of reverberation artifacts, suggesting presence of gas. These two structures were excised during a laparotomy and Clostridium tertium was isolated. To the best of our knowledge, this is the first report of C. tertium infection in a pet animal. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Clostridia; Granuloma; Diagnostic imaging. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000100551 |
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Rodríguez,H; Espinoza-Navarro,O; Peñafiel,C; Escobar,E; Silva,I; Quiroz,P; Arriaza,C; Castro,M. E; Sánchez,C; Arias,M; Michea,A. |
Activation of macrophages in periapical granulomas occurs through the presence of cytokines, endotoxin and other genetic and epigenetic factors, allowing the initiation of inflammation and bone resorption. The present study aims to analyze the presence of CD133 protein (marker of stem cells) and the AR (androgen receptor) protein in biopsies of human odontogenic periapical granuloma. Biopsies from 14 adult male patients with diagnosis of periapical granuloma included in paraffin blocks were processed histologically to obtain 5-µm thick sections. Protein presence was detected and analyzed by immunohistochemistry of CD133 and AR. The quantification considered the number of positive cells in 0.17 mm2 random areas under the microscope using a 1000X objective.... |
Tipo: Journal article |
Palavras-chave: Stem cell; Androgen receptor; Granuloma; Periodontitis. |
Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000300048 |
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Sette,Christiane Salgado; Wachholz,Patrick Alexander; Masuda,Paula Yoshiko; Figueira,Renata Borges Fortes da Costa; Mattar,Fernanda Rodrigues de Oliveira; Ura,Deise Godoy. |
AbstractThe infection by Mycobacterium marinum in humans is relatively uncommon. When it occurs, it mainly affects the skin, usually with a chronic, indolent and benign evolution. The diagnosis requires a high index of suspicion, and a significant delay may be observed between the first symptoms to the final diagnosis. This present case reports a M. marinum infection in an immunocompetent patient that had a chronic undiagnosed injury on the dominant hand for at least five years. The patient had several medical consultations, without proper suspicion, hampering adequate diagnostic investigation. Histopathology detected tuberculoid granulomas, but showed no acid-fast bacilli. The culture in appropriate medium and the polymerase chain reaction-restriction... |
Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Mycobacterium infections; Mycobacterium marinum; Case report; Granuloma. |
Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100412 |
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Silveira,Eliane F. da; Amato,José F. R.; Amato,Suzana B.. |
This is the first report of a species of Echinuria Soloviev, 1912, on anatid hosts in South America, causing granulomas. It is also the first detailed description and record of a species of Echinuria, for South America in the Rosy-billed Pochard, Netta peposaca (Vieillot, 1816). Fifty-two rosy-billed pochards were examined for helminths in the Municipalities of Santa Vitória do Palmar (locality of Fazenda Sossego) and (locality of Ponta da Antena), State of Rio Grande do Sul, Brazil, and Alvear, Province of Corrientes, northern Argentina. Samples were obtained from 2003 to 2004. After the catch each bird was immediately frozen with dry ice. Prior to necropsy birds were sexed, weighted, measured, and the state of maturity (juvenile or adult) determined,... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Breeding grounds; Granuloma; Migratory flyway; Northern Argentina; Rosy-billed pochard; Southern Brazil; Wintering grounds. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752006000200027 |
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Registros recuperados: 15 | |
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