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Effect of supplementation of green tea polyphenols on the developmental competence of bovine oocytes in vitro BJMBR
Wang,Z.G.; Yu,S.D.; Xu,Z.R..
The objective of the present study was to examine the effect of green tea polyphenols (GTPs) supplementation during in vitro maturation, in vitro fertilization, and in vitro culture on the developmental competence of bovine oocytes. Cumulus-oocyte complexes aspirated from the ovaries were matured in vitro (38.5ºC for 24 h) and fertilized (38.5ºC for 15-18 h) and embryos were cultured (38.5ºC for 192 h) in a defined conditioned medium with or without GTPs supplementation. The GTPs used in the present study contained 99% catechin derivatives, with the major components being 50% (-)-epigallocatechin gallate, 22% (-)-epicatechin gallate, 18% (-)-epigallocatechin, and 10% (-)-epicatechin. Four replicate trials were done for each type of experiment. GTPs...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine oocytes; In vitro culture; Green tea polyphenols; Embryo development.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800008
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Optimization and release evaluation for tea polyphenols and chitosan composite films with regulation of glycerol and Tween Ciênc. Tecnol. Aliment.
PENG,Yong; WANG,Qingguo; SHI,Jingying; CHEN,Yilun; ZHANG,Xiaoyan.
Abstract In order to optimize the performance of chitosan-tea polyphenols composite films, effects of glycerol and Tween on the antioxidative activities and physical performance of chitosan composite films were evaluated. The results indicated that both flexibility and water vapour permeability of chitosan/tea polyphenols films were improved with the addition of glycerol, meanwhile the solubility and swelling degree decreased. Moreover, the release time of antioxidants from films was delayed with the addition of glycerol. The presence of Tween 20 had no significant effect on the tensile strength of films, but changed the DPPH radical scavenging activity depending on the blend ratio of glycerol and Tween. DSC, FTIR and X-ray analysis showed strong...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chitosan film; Green tea polyphenols; Optimization; Antioxidant ability; X-ray diffraction.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612020000100162
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