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| SPERA,KAMILLE D.; FIGUEIREDO,PATRÍCIA A.; SANTOS,PAMELA C.E; BARBOSA,FERNANDO C.; ALVES,CAIO P.; DOKKEDAL,ANNE LÍGIA; SALDANHA,LUIZ LEONARDO; SILVA,LUCIANA P.; FIGUEIREDO,CARLOS ROGÉRIO; FERREIRA,PAULO CESAR; SILVA,REGILDO M.G. DA. |
| Abstract: Hymenaea courbaril has been used to treat different diseases, although its properties are yet to be scientifically validated. The objective of this study was to determine the cytotoxicity, genotoxicity, antigenotoxicity and antioxidant potentials of hydroethanolic extract from H. courbaril seeds. Therefore, for the cytotoxicity test an anti-melanoma assay was performed in B16F10 strain cells. The genotoxicity and antigenotoxicity was evaluated in bone marrow cells (Permit number: 002/2010) of mice, the antioxidant activity was determined by the DPPH test and the total flavonoid content was also determined. The hydroethanolic extract showed antigenotoxic effect and antioxidant activity. It was verified that total flavonoid content was 442.25±18.03... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: B16F10 melanoma cells; Micronucleus test; Chromosomal aberration; DPPH radical scavenging; HPLC-DAD. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000700617 |
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| Machado,Simone Caetani; Landin-Silva,Mariane; Maia,Patrícia Penido; Rath,Susanne; Martins,Isarita. |
| The development of a QuEChERS-HPLC-DAD method using a Lichrospher 60 RP-Select B column (250 x 4.6 mm x 5 µm) at 40ºC, mobile phase constituted by phosphate buffer:acetonitrile (75:25, v/v) at a initial flow rate of 0.5 mL min-1, increased by 1.2 mL min-1 and at 265 nm is presented for simultaneous determination of sulphadiazine, sulphametoxipiridazine and sulphamethoxazole in chicken breast samples. QuEchERS is inexpensive, fast and easy, and the extraction of the analytes of the matrix was successfully employed. In addition, the method presented linearity, in the range of 25, 50, 100, 150, 175, and 200 µg kg-1, precision, selectivity and sensitivity. The intraday precision (RSD %) for QuEChERS method was between 3.6-10.8 (SDZ), 6.9-14.1 (SPZ) and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: QuEChERS; Sulphonamides determination; Veterinary drugs residues; Chicken breast; HPLC-DAD. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000100017 |
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| Pedroso,Rita Cássia Nascimento; Branquinho,Núbia Angélica de Ávila; Hara,Alessandra Cristina Boffino de Almeida Monteiro; Silva,Fabiano Guimarães; Kellner Filho,Luis Claudio; Silva,Marcio Luís Andrade; Cunha,Wilson Roberto; Pauletti,Patricia Mendonça; Januário,Ana Helena. |
| ABSTRACT: Hyptis marrubioides (Lamiaceae) is a medicinal plant that is native from Brazilian Cerrado. In vitro propagation techniques make use of elicitors to alter metabolic pathways, affecting how molecules are produced both qualitatively and quantitatively. This research aimed to evaluate how abiotic elicitors salicylic acid (SA) and silver nitrate (SN) at concentrations of 30µM or 60µM influence Hyptis marrubioides seedling growth by two different in vitro culture methods. The rutin content was quantified by HPLC-DAD. Compared to an untreated culture, the H. marrubioides methanolic extracts cultured in MS medium for 10 days followed by culture in MS medium containing SN (30µM) for 20 days had 1.28 times higher rutin content. In a second experiment,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salicylic acid; HPLC-DAD; Tissue culture; Silver nitrate; Elicitation; Plant growth regulators. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000200400 |
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