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On-line HPLC analysis of the antioxidant activity of phenolic compounds in brewed, paper-filtered coffee Braz. J. Plant Physiol.
Stalmach,Angélique; Mullen,William; Nagai,Chifumi; Crozier,Alan.
Caffeoyl-, feruloyl- and dicaffeoylquinic acids (chlorogenic acids) in infusions from green and medium roasted coffee beans were identified and quantified by reverse phase HPLC with photodiode array and MS³ detection prior to assessment of the antioxidant activity using an HPLC system with post-column on-line antioxidant detection based on 2,2'-azinobis-3-ethylbenzothiazoline-6-sulphonic acid radical scavenging activity. Caffeoylquinic acids were the most abundant antioxidants and roasting induced isomerisation with a decline in 5-O-caffeoylquinic acid and concomitant increases in the 3- and 4-O-derivatives. This did not affect the level of caffeoylquinic acid-derived antioxidant activity in the roasted coffee. Roasting did, however, result in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coffea; ABTS+; Chlorogenic acids; Hydroxycinnamates; HPLC-MSn.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000100018
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