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Fine cytochemical localization of lysosomal organelles in phagocytic cells of bivalve molluscs. ArchiMer
Auffret, Mathieu; Diouris, M; Herry, A.
Bivalve molluscs possess several types of hemolymph cells (hemocytes), involved in internal defense and other physiological functions. Studies in pathological and experimental conditions have shown their micro- and macrophagic ability. However, the mechanisms of intracellular digestion are incompletely known in these organisms. The lysosomal apparatus of hemocytes was therefore investigated in several species by hydrolase localization with the electron microscope. The cytoplasmic granules of granulocytes displayed a strong acid beta -glycerophosphatase activity. Previous observations had shown that these granules may contain phagocytized material. This indicates that intracellular digestion is achieved in these organelles. Acid beta -glycerophosphatase...
Tipo: Text Palavras-chave: Bivalvia; Phagocytosis; Cells; Ultrastructure; Haemolymph.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/1991/acte-1631.pdf
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Trophic relationships gonad-intestinal loop in Pecten maximus . ArchiMer
Lepennec, M; Dorange, G; Beninger, P; Donval, A; Widowati, I.
Histological, histochemical and ultrastructural studies were performed on the region of the gonad traversed by the intestine in Pecten maximus . The results indicate a digestive and absorptive function of intestine epithelial cells and a transfer of metabolites to the surrounding gonad tissue. Several possible mechanisms of translocation are presented, including diffusion into the haemolymph and transfer via vacuolated cells of the gonad interstitial connective tissue.
Tipo: Text Palavras-chave: Pecten maximus; Bivalvia; Histology; Haemolymph; Histochemistry; Cells; Metabolites; Epithelia; Intestines; Ultrastructure; Animal reproductive organs.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/1991/acte-1668.pdf
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In vitro research of anti-HSV-1 activity in different extracts from Pacific oysters Crassostrea gigas ArchiMer
Olicard, Cecile; Didier, Yohann; Marty, Christel; Bourgougnon, Nathalie; Renault, Tristan.
Mortalities related to the detection of Ostreid Herpesvirus 1 (OsHV-1) have been previously reported in France among larvae and spat of the Pacific oyster Crassostrea gigas. Adult oysters appear less sensitive to herpesvirus infections, although OsHV-1 has been detected in adults without signs of disease or mortality. This suggests that the virus is able to persist in its host and that adult oysters may be able to control OsHV-1 infection. Little is known about antiviral substances in invertebrates. The present work concerns the research of antiviral substances in adult oyster C. gigas, where putative antiviral activities were monitored using 3 strategies: (1) in metabolites with variable polarity, (2) in peptidic extracts and (3) in crude haemolymph. In...
Tipo: Text Palavras-chave: Haemolymph; Antiviral peptides; HSV 1; OsHV 1; Antiviral defence; Crassostrea gigas; Oyster.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-2922.pdf
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Caracterisation des hemocytes d''un mollusque bivalve marin, la nacre, Pinna nobilis L. 1758 ArchiMer
Henry, M; Vicente, N; Houache, N.
The study of tinctorial affinities, in vitro behaviour and ultrastructure of the hemocytes of Pinna nobilis allows us to group them into two main families: basophilic, neutrophilic and acidophilic granulocytes; hyalinocytes, three categories can be distinguished among these cells: small hyalinocytes, hyalinocytes containing smooth endoplasmic reticulum and the macrophage population. The hemocytes participate in the regulation of metabolism by the storage and degradation of glycogen. They contribute to the detoxification process by the sequestration of mineral elements in their lysosomes. Their role in antibacterial defense is suggested by their bacterian phagocytic activity.
Tipo: Text Palavras-chave: Pinna nobilis; Bivalvia; Haemolymph; Animal metabolism; Marine molluscs; Ultrastructure.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/1991/acte-1711.pdf
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Growth factors in Penaeid shrimp feeding ArchiMer
Guillaume, Julien; Cruz, R; Cuzon, Gerard; Wormhoudt Van, A; Revol, A.
Some nutrients known as non essential in vertebrates could be essential in invertebrates and could be related to some "unknown growth factors" which were evidenced or suspected, mainly in mollusc or crustacean flesh or meal, by several authors working on different species of crustaceans. A set of experiments was carried out in our laboratory in order to isolate one of these factors found in squid meal.
Tipo: Text Palavras-chave: Penaeus japonicus; Food absorption; Digestive system; Haemolymph; Amino acids; Glucose; Digestion; Enzymes; Experimental research; Feeding; Shrimp culture; Laboratory culture.
Ano: 1989 URL: http://archimer.ifremer.fr/doc/1989/acte-1464.pdf
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Haemolymph electrophoretic pattern of Ascia monuste orseis larvae (Lepidoptera: pieridae) parasitized by Cotesia glomerata (Hymenoptera: braconidae) J. Venom. Anim. Toxins incl. Trop. Dis.
Scaglia,M.; Brochetto-Braga,M. R.; Chaud-Netto,J.; Gobbi,N..
Cotesia glomerata is a natural enemy of the vegetable plague Ascia monuste orseis and preferably parasites 2nd, 3rd and 4th instar larvae. Parasitism effects on the haemolymph protein profile of Ascia monuste orseis larvae from the 2nd to 7th days were analyzed both qualitatively and quantitatively by SDS-PAGE and Coomassie-Blue binding methods. Quantitative analysis showed a progressive increase in the protein content of about 6.5 and 12.5 times in parasitized and non-parasitized larvae from the 2nd to 5th days, respectively. On the 6th day, a decrease in protein content was observed in both groups, although this decrease was significantly less than the control group that continued to metamorphosis. Meanwhile, parasitized larvae had one more day (7th day)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ascia monuste orseis; Cotesia glomerata; Electrophoretic protein profile; Haemolymph; Natural enemy; Parasitism; SDS-PAGE.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000100006
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