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	Provedor de dados BJM (2) BABT (1) Autor Abraham,Theruvath Koshy (1) Bhadekar,Rama (1) DeSa,Elisha (1) Fulzele,Rachana (1) Li,Xin (1) Nair,Ananthakrishnan Jayakumaran (1) Prasad,Roopa (1) Shouche,Yogesh (1) Wang,Chuan-Xu (1) Yadav,Amit (1) Mais... Palavra-chave Halotolerant (3) Endoprotease (1) Lytic phage (1) Mesophilic (1) Neutral protease (1) Poultry soil (1) Scale up fermentation (1) Spherical (1) Thermostable (1) Wheat bran (1) Yuncheng saline lake (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2018 (1) 2014 (1) 2011 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Scale up of production in a bioreactor of a halotolerant protease from moderately halophilic Bacillus sp. isolated from soil BABT Prasad,Roopa; Abraham,Theruvath Koshy; Nair,Ananthakrishnan Jayakumaran. Studies were conducted on the production of protease by moderately halophilic Bacillus sp. on agro-industrial waste materials. The bacterium could efficiently use many agro wastes as substrates but wheat bran supported maximum enzyme production. To ascertain the performance of the process in shake flasks and lab scale bioreactor, experiments were conducted to analyse protease activity utilizing wheat bran as cost effective substrate. The studies unveiled that pH 7.0, temperature 30°C and static conditions were optimal for enzyme production in flask level fermentation. In scale-up fermentation, at optimal pH and temperature, agitation rate of 50 rpm was best for protease production. The enzymatic nature was studied in 10% SDS gels with BSA (2.5 mg/mL) as... Tipo: Info:eu-repo/semantics/article Palavras-chave: Poultry soil; Endoprotease; Scale up fermentation; Wheat bran; Halotolerant. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300020 JMT-1: a novel, spherical lytic halotolerant phage isolated from Yuncheng saline lake BJM Wang,Chuan-Xu; Li,Xin. ABSTRACT This work described a novel halotolerant phage, JMT-1, with a spherical morphology. JMT-1, which was isolated from a hypersaline lake, could produce clear plaques on Chromohalobacter sp. LY7-3. The purified virions are spherical, have no visible tail, and are about 30–50 nm in diameter. JMT-1 has a wide host range, and this study showed that the phage can infect at least five halophilic bacteria. The proteins of JMT-1 were analyzed using sodium dodecyl sulfate polyacrylamide gel electrophoresis, and six proteins were detected. Results show that JMT-1 is a bacteriophage with a linear double-stranded DNA. Meanwhile, the genome is approximately 23 kb in length and is sensitive to the restriction endonucleases Bam I, EcoR I, Hind III and Kpa I. JMT-1... Tipo: Info:eu-repo/semantics/article Palavras-chave: Lytic phage; Halotolerant; Spherical; Yuncheng saline lake. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500262 Characterization of novel extracellular protease produced by marine bacterial isolate from the Indian Ocean BJM Fulzele,Rachana; DeSa,Elisha; Yadav,Amit; Shouche,Yogesh; Bhadekar,Rama. Out of the vast pool of enzymes, proteolytic enzymes from microorganisms are the most widely used in different industries such as detergent, food, peptide production etc. Several marine microorganisms are known to produce proteases with commercially desirable characteristics. We have isolated nine different cultures from marine samples of the Indian Ocean. All of them were i) motile ii) rod shaped iii) non spore forming iv) catalase and amylase positive v) able to grow in presence of 10 % NaCl. They produced acid from glucose, fructose and maltose and grew optimally at 30 0C temperature and pH 7.0-8.0. None of them could grow above 45 0C and below 15 0C. Only one of them (MBRI 7) exhibited extracellular protease activity on skim milk agar plates. Based on... Tipo: Info:eu-repo/semantics/article Palavras-chave: Halotolerant; Mesophilic; Neutral protease; Thermostable. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400018 Registros recuperados: 3 Primeira ... 1 ... Última

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