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| Macedo,Nicodemos Alves de; Morais,Zenáide Maria de; Camargo,Cláudio Roberto de Almeida; Alves,Clebert José; Azevedo,Sérgio Santos de; Nümberger Júnior,Rodolfo; Vasconcellos,Sílvio Arruda. |
| Foi investigada a influência da via de inoculação sobre o estabelecimento e a evolução da leptospirose em hamsters (Mesocricetus auratus) experimentalmente infectados com Leptospira interrogans sorovar pomona. As vias de inoculação ensaiadas foram: intraperitoneal, subcutânea, oral, conjuntival e escarificação cutânea. O inóculo infeccioso foi constituído por uma cultura em meio de Fletcher, com 20 a 30 leptospiras por campo microscópio no aumento de 200 vezes. Os animais controle foram inoculados apenas com meio de Fletcher. Foram colhidas amostras de soro sanguíneo e fragmentos de rins na fase agônica da doença ou no 21º dia pós-infecção, quando todos os animais foram sacrificados. Para a pesquisa de leptospiras, foi feito o exame direto com microscopia... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Via de inoculação; Hamsters; Sorovar pomona. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962004000300008 |
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| Tanaka,D.M.; Romano,M.M.D.; Carvalho,E.E.V.; Oliveira,L.F.L.; Souza,H.C.D.; Maciel,B.C.; Salgado,H.C.; Fazan-Júnior,R.; Simões,M.V.. |
| Determination of left ventricular ejection fraction (LVEF) using in vivo imaging is the cardiac functional parameter most frequently employed in preclinical research. However, there is considerable conflict regarding the effects of anesthetic agents on LVEF. This study aimed at assessing the effects of various anesthetic agents on LVEF in hamsters using transthoracic echocardiography. Twelve female hamsters were submitted to echocardiography imaging separated by 1-week intervals under the following conditions: 1) conscious animals, 2) animals anesthetized with isoflurane (inhaled ISO, 3 L/min), 3) animals anesthetized with thiopental (TP, 50 mg/kg, intraperitoneal), and 4) animals anesthetized with 100 mg/kg ketamine plus 10 mg/kg xylazine injected... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Echocardiography; Anesthetics; Systolic function; Hamsters. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001000602 |
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| Tabata,Rosana; Scanavini Neto,Hugo; Zuanaze,Marcelo Alexandre Fagnani; Oliveira,Eugenia Márcia de Deus; Dias,Ricardo Augusto; Morais,Zenaide Maria de; Ito,Fumio Honma; Vasconcellos,Silvio Arruda. |
| Three leptospiral bacterins, produced with different serovars of Serogroup Sejroe, namely the hardjo (bacterin A), wolffi (bacterin B) and guaricura (bacterin C), were evaluated in male hamsters (Mesocricetus auratus) by comparing the agglutinating and neutralizing antibodies titers using microscopic agglutination (MAT) and in vitro growth inhibition (GIT) tests. The immunization schedule was based on two 1.0 mL doses of non-diluted formalininactivated whole culture bacterin given through subcutaneous route with 10-day interval. The challenge was performed ten days after the second vaccine dose, when the animals were inoculated with 0.2 mL of non-inactivated cultures of each serovar through intraperitoneal route. On the 21st post-challenge day (PCD), all... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospira; Bacterins; Hamsters; Neutralizing antibodies; Serogroup Sejroe. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000300016 |
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| Dib,Cristina Corsi; Gonçales,Amane Paldês; Morais,Zenaide Maria de; Souza,Gisele Oliveira de; Miraglia,Fabiana; Abreu,Patricia Antonia Estima; Vasconcellos,Silvio Arruda. |
| We investigated the existence of cross-protection between two anti-leptospirosis monovalent experimental bacterins produced with two strains of Leptospira serogroup Pomona: Fromm strain of serovar Kennewicky, isolated from pigs in the United States, and strain GR6 of serovar Pomona isolated from pigs in Brazil. Both were added of aluminum hydroxide as an adjuvant. Experimental bacterins were tested with the hamster potency test in order to assess protection provided against the disease and against the establishment of kidney infection. Controls were polyvalent commercial vaccine produced with Leptospira strains isolated outside Brazil, which included a representative of Pomona serovar, or Sorensen solution added of aluminum hydroxide adjuvant. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pomona; Hamsters; Bacterin; Cross-protection. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300042 |
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