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Melo,V.M.M.; Fonseca,A.M.; Vasconcelos,I.M.; Carvalho,A.F.F.U.. |
The antimicrobial, hemagglutinating and toxic activities of the purple fluid of the sea hare Aplysia dactylomela are described. Intact or dialyzed purple fluid inhibited the growth of species of Gram-positive and Gram-negative bacteria and the action was not bactericidal but bacteriostatic. The active factor or factors were heat labile and sensitive to extreme pH values. The fluid preferentially agglutinated rabbit erythrocytes and, to a lesser extent, human blood cells, and this activity was inhibited by the glycoprotein fetuin, a fact suggesting the presence of a lectin. The fluid was also toxic to brine shrimp nauplii (LD50 141.25 µg protein/ml) and to mice injected intraperitoneally (LD50 201.8 ± 8.6 mg protein/kg), in a dose-dependent fashion. These... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aplysia; Antimicrobial; Purple fluid; Toxicity; Hemagglutinating activity. |
Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600010 |
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Mikcha,Jane M.G.; Ferreira,Antonio J. Piantino; Ferreira,Claudete S. Astolfi; Yano,Tomomasa. |
Twenty-five strains of Salmonella enterica serovar Enteritidis isolated from different sources were examined for hemagglutinating activity. Bacteria cultured in different media induced hemagglutination of human erythrocytes, but no reaction was observed with erythrocytes from other animal species. The hemagglutinating expression activity was better for cultures on CFA agar at 37ºC than other conditions examined. The hemagglutination was inhibited by D-mannose, D-mannitol, melibiose, D-raffinose, L-rhamnose and sucrose. The absence of cell-surface appendages in electron microscope examinations suggested a nonfimbrial hemagglutinin. The data suggest that Salmonella Enteritidis produces nonfimbrial mannose-sensitive hemagglutinin, specific for human... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella Enteritidis; Hemagglutinating activity; Mannose-sensitive hemagglutinin; Nonfimbrial hemagglutinin. |
Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100008 |
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