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Registros recuperados: 10 | |
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Labate,Mônica T. Veneziano; Bertolo,Ana L. Ferreira; Nascimento,Daniela Defávari do; Gutmanis,Gunta; Andrade,Alexander de; Rodrigues,Maria J. Calderan; Camargo,Eduardo L.O.; Boaretto,Luis Felipe; Moon,David H.; Bragatto,Juliano; Labate,Carlos A.. |
UDP-glucose dehydrogenase (UGDH) catalyzes the oxidation of UDP-glucose (UDP-Glc) to UDP-glucuronate (UDP-GlcA), a key sugar nucleotide involved in the biosynthesis of plant cell wall polysaccharides. A full-length cDNA fragment coding for UGDH was cloned from the cambial region of 6-month-old E. grandis saplings by RT-PCR. The 1443-bp-ORF encodes a protein of 480 amino acids with a predicted molecular weight of 53 kDa. The recombinant protein expressed in Escherichia coli catalyzed the conversion of UDP-Glc to UDP-GlcA, confirming that the cloned cDNA encodes UGDH. The deduced amino acid sequence of the cDNA showed a high degree of identity with UGDH from several plant species. The Southern blot assay indicated that more than one copy of UGDH is present... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: UDP-glucose; UDP-glucuronate; Hemicellulose; Pectin; Cell wall. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400015 |
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Omori,Wellington P.; Pinheiro,Daniel G.; Kishi,Luciano T.; Fernandes,Camila C.; Fernandes,Gabriela C.; Gomes-Pepe,Elisângela S.; Pavani,Claudio D.; Lemos,Eliana G. de M.; Souza,Jackson A. M. de. |
Abstract The filter cake from sugar cane processing is rich in organic matter and nutrients, which favors the proliferation of microorganisms with potential to deconstruct plant biomass. From the metagenomic data of this material, we assembled a draft genome that was phylogenetically related to Thermomonospora curvata DSM 43183, which shows the functional and ecological importance of this bacterium in the filter cake. Thermomonospora is a gram-positive bacterium that produces cellulases in compost, and it can survive temperatures of 60 ºC. We identified a complete set of biomass depolymerizing enzymes in the draft genome of Thermomonospora sp. CIT 1, such as α-amylase, catalase-peroxidases, β-mannanase, and arabinanase, demonstrating the potential of this... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Actinobacteria; Pectin; Hemicellulose; Crystalline cellulose; Orthologous genes. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100145 |
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Vitorino,Patrícia Goulart; Alves,José Donizeti; Magalhães,Paulo César; Magalhães,Marcelo Murad; Lima,Luíz Carlos Oliveira; Oliveira,Luiz Edson Mota de. |
This research aimed to characterize the tolerance to flooding and alterations in pectic and hemicellulose fractions from mesocotyl of maize tolerant to flooding when submitted to hypoxia. In order to characterize tolerance seeds from maize cultivars Saracura BRS-4154 and BR 107 tolerant and sensitive to low oxygen levels, respectively, were set to germinate. Plantlet survival was evaluated during five days after having been submitted to hypoxia. After fractionation with ammonium oxalate 0.5% (w/v) and KOH 2M and 4M, Saracura BRS-4154 cell wall was obtained from mesocotyl segments with different damage intensities caused by oxygen deficiency exposure. The cell wall fractions were analyzed by gel filtration and gas chromatography, and also by Infrared... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pectic substances; Hemicellulose. |
Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2001000800004 |
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Medeiros,Lorena L. de; Silva,Flávio L. H. da; Santos,Sharline F. M.; Madruga,Marta S.; Melo,Débora J. N. de; Conrado,Líbia de S.. |
ABSTRACT The agro-industrial waste deposited in the environment causes problems in nature that can be solved with the use and generation of bioproducts. Thus, the objective was to study the lignocellulosic fraction of cashew (Anacardium occidentale L.) peduncle bagasse and fermentation on large scale (8-16 times) using the strain Candida guilliermondii CCT-3544 as production agent. According to the obtained results, it can be noted that the dry cashew peduncle bagasse has 21.45% of cellulose, 10.96% of hemicellulose and 35.39% of lignin. During fermentation, C. guilliermondii 3544-CAT was able to grow on medium containing hydrolysate, with maximum cell growth concentration of 3.5 g L-1. The behavior of the sugars in the fermentation process was similar in... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anacardium occidentale L.; Candida guilliermondii; Hemicellulose; Bioproduction. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662017000700488 |
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Registros recuperados: 10 | |
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