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The value of PCR-RFLP molecular markers for the differentiation of immature stages of two necrophagous flies (Diptera: Calliphoridae) of potential forensic importance Neotropical Entomology
Thyssen,Patricia J.; Lessinger,Ana C.; Azeredo-Espin,Ana M.L.; Linhares,Arício X..
The identification of insect species involved in corpses decomposition is of particular importance in estimating the post-mortem interval (PMI) in forensic science, since the PMI is based on information about the life cycle of necrophagous insects. However, the identification of some insect species, especially in their immature stages, may be complicated by many factors, even for experienced taxonomists. Species of the same genus such as Hemilucilia segmentaria (Fabricius) and H. semidiaphana (Rondani) (Diptera: Calliphoridae) are morphologically and behaviorally very similar, but differ in their growth and maturation rates. These species are abundant in forests, exclusively necrophagous and, therefore, are of potential medicolegal importance for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Forensic entomology; Mitochondrial DNA; Hemilucilia segmentaria; Hemilucilia semidiaphana.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2005000500009
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First description of the immature stages of Hemilucilia segmentaria (Diptera: Calliphoridae) Biol. Res.
THYSSEN,PATRICIA J; LINHARES,ARÍCIO X.
The immature stages oí Hemilucilia segmentaria (Fabricius, 1805) (Diptera: Calliphoridae) are described. Egg morphology and structures such as the cephalopharyngeal skeleton, anterior and posterior spiracles, and the dorsal spines between the prothorax and mesothorax from first, second and third instar larvae are characterized, using light and scanning electron microscopy. This species is abundant in Neotropical forests and, because of its necrophagous behavior, is of substantial medico-legal importance for estimating the postmortem interval in criminal investigations. Information presented herein may be useful to differentiate among eggs and larvae of closely related species and to supplement the database for blowfly identification
Tipo: Journal article Palavras-chave: Hemilucilia segmentaria; Calliphoridae; Immature stages; Forensic Entomology.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000400001
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