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Assessment of age and greenness of herbarium specimens as predictors for successful extraction and amplification of DNA Naturalis
Erkens, R.H.J.; Cross, H.; Maas, J.W.; Hoenselaar, K.; Chatrou, L.W..
Age and the greenness of leaves have been frequently used as indicators for selecting herbarium specimens for molecular studies. Although plant DNA extraction and amplification have been common lab procedures for the past 20 years, no studies specifically investigated the success of these indicators. Here the predictive value of age and the greenness for extraction and amplification success is assessed, using a large number of herbarium specimens from different plant groups. The investigation of these indicators is important because herbarium material is a precious commodity, and is often the only remaining floral record of now extinct ecosystems. In cases where little leaf material is available, most researchers still attempt to extract DNA. This study...
Tipo: Article / Letter to the editor Palavras-chave: Best laboratory practice; Herbarium specimen collection and selection; Guatteria; Zehneria; Rauwolf herbarium.
Ano: 2008 URL: http://www.repository.naturalis.nl/record/525744
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