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| Mathew,Ceema; Swathi,Bellamkonda; Ajitha,Makula; Babu,Puvvadi Sathesh. |
| ABSTRACT The present work describes the development of a new high performance liquid chromatographic (HPLC) method for the determination of Cefixime trihydrate under different stress conditons as specified by ICH. For the analysis, a Phenomenex (250 x 4.6 mm, 5 µm particle size) ODS column and a SPD 20 A UV detector at 289 nm was used. The selected mobile phase was 10 mM disodium hydrogen phosphate (with 0.5% TEA, pH adjusted to 6.3 with OPA) and methanol in the ratio of 75:25 (v/v) in isocratic mode at a flow rate of 1 mL.min-1.The linear regression analysis data for the calibration plots showed good linear relationship with r2 = 0.9997 in the concentration range of 5-100 μg.mL-1. The stress degradation was performed using acid, alkali, water, hydrogen... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: High Performance Liquid Chromatography/quantitative analysis; Degradation; Stability-indicating; Cefixime trihydrate/determination.. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000100010 |
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| Marinho,Pablo Alves; Leite,Edna Maria Alvarez. |
| In the present study, a method using high performance liquid chromatography to quantify LSD, in blotter papers seized in Minas Gerais, was optimized and validated. Linearity, precision, recovery, limits of detection and quantification, and selectivity were the parameters used to evaluate performance. The samples were extracted with methanol:water (1: 1) in an ultra-sound bath. The linearity between 0.05 and 20.00 μg/mL (0.5 and 200.0μg of LSD/blotter) was observed with satisfactory mean intra and inter assay precision (RSDr = 4.4% and RSD R = 6.4%, respectively) and with mean recoveries of 83.4% and 84.9% to the levels of 1.00 and 20.00 μg/mL (10 and 200μg LSD/blotter). The limits of detection and quantification were 0.01 and 0.05 μg/mL, respectively (0.1... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: LSD/detection; Forensic chemistry; Analytical toxicology; In-house validation; High Performance Liquid Chromatography/quantitative analysis. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400011 |
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| Santos,Carolina Alves dos; Oliveira-Nascimento,Laura; Knirsch,Marcos Camargo; Stephano,Marco Antônio; Pessoa Júnior,Adalberto; Penna,Thereza Christina Vessoni. |
| Ceftazidime is a broad spectrum antibiotic administered mainly by the parenteral route, and it is especially effective against Pseudomonas aeruginosa. The period of time in which serum levels exceed the Minimum Inhibitory Concentration (MIC) is an important pharmacodynamic parameter for its efficacy. One of the forms to extend this period is to administer the antibiotic by continuous infusion, after prior dilution in a Parenteral Solution (PS). The present work assessed the stability of ceftazidime in 5% glucose PS for 24 hours, combined or not with aminophylline, through High Performance Liquid Chromatography (HPLC). The physicochemical evaluation was accompanied by in vitro antimicrobial activity compared MIC test in the 24-hour period. Escherichia coli... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Drug/interactions; Injectables; Parenteral solutions; Ceftazidime/stability; Aminophylline/stability; High Performance Liquid Chromatography/quantitative analysis; Minimum inhibitory concentration (MIC). |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000400013 |
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| Ashfaq,Muhammad; Akhtar,Tazeem; Mustafa,Ghulam; Danish,Muhammad; Razzaq,Syed Naeem; Nazar,Muhammad Faizan. |
| A viable cost-effective and isocratic approach employing C-18 column (250 mm × 4.6 mm, 5 µm) based HPLC has been utilized to separate and estimate the drugs, rosuvastatin, amlodipine and their stress induced degradation products, simultaneously in pharmaceutical formulations. Focused on ICH guideline parameters, the efficient separation of both drugs and their degradation products was achieved by optimizing a 30:70 (v/v) solvent mixture of acetonitrile and 0.1 M ammonium acetate buffer (pH 5) as mobile phase. The flow rate of the mobile phase was 1.5 mL/min and all the detections were carried out at 240 nm using UV detector. The method was linear in the concentration range of 1-200 µg/mL for rosuvastatin with 0.996 coefficient of determination value. For... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: High Performance Liquid Chromatography/quantitative analysis; Rosuvastatin/quantitative determination; Amlodipine/quantitative determination; Pharmaceutical formulations/quantitative analyis; Acetonitrile; UV Detector. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000300629 |
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