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Registros recuperados: 58
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Corona development and floral nectaries of Asclepiadeae (Asclepiadoideae, Apocynaceae) 39
Monteiro,Mariana Maciel; Demarco,Diego.
ABSTRACT Flowers of Asclepiadoideae are notable for possessing numerous nectaries and elaborate coronas, where nectar can accumulate but is not necessarily produced. Given the complexity and importance of these structures for reproduction, this study aimed to analyze the ontogeny of the corona, the structure and position of nectaries and the histochemistry of the nectar of species of Asclepiadeae. Two types of coronas were observed: androecial [C(is)] and corolline (Ca). The development of the C(is)-type of corona initiates opposite the stamens in all species examined with the exception of Matelea in which it begins to develop as a ring around the filament tube. Despite their morphological variation, coronas typically originate from the androecium. A...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Asclepiads; Histochemistry; Ontogeny; Nectar; Structural; Diversity.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000300420
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Morphology and viability of pollen grains from passion fruit species (Passiflora spp.) 39
Soares,Taliane Leila; Jesus,Onildo Nunes de; Souza,Everton Hilo de; Santos-Serejo,Janay Almeida dos; Oliveira,Eder Jorge de.
The characterization and viability of pollen grains are useful tools to guide crosses in breeding programs. The objective of this study was to describe the morphological patterns and viability of pollen grains from five accessions of Passiflora edulis f. flavicarpa O. Deg. and five accessions of Passiflora setacea DC. Pollen morphology descriptions were made using light microscopy and scanning electron microscopy, whereas the viability analysis was performed by in vitro germination and histochemical analysis (Lugol's solution and 2,3,5-triphenyltetrazolium chloride). Pollen grains assessed for germination were inoculated in culture medium containing Ca(NO3).4H2O (0.03%), Mg(SO4).7H2O (0.02%), KNO3 (0.01%), H3BO3 (0.01%), sucrose (15%) and agar (0.8%)....
Tipo: Info:eu-repo/semantics/article Palavras-chave: In vitro germination; Histochemistry; Scanning electron microscopy; Passifloraceae; Pollen tube.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062013000400018
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Comparative anatomy of calyx and foliar glands of Banisteriopsis C. B. Rob. (Malpighiaceae) 39
Araújo,Josiane Silva; Meira,Renata Maria Strozi Alves.
Banisteriopsis is considered one of the largest genera of Malpighiaceae with 58 species, of which 47 occur in Brazil. The typical calyx and leaf glands of Banisteriopsis are considered relevant to the adaptive success of Malpighiaceae. Comparative studies of anatomical and histochemical characteristics may reveal similarities and assist in the interpretation of the functions performed by such glands. The present study aimed to describe the anatomy of the calyx and leaf glands of 38 species ofBanisteriopsis that occur in Brazil, and to analyze these structures histochemically in B. campestris, B. laevifolia and B malifolia, using standard methods. Calyx glands differ from leaf glands by possessing an irregular surface that is covered by a thick cuticle that...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Banisteriopsis; Calyx glands; Histochemistry; Leaf glands; Lipids; Micromorphology; Polysaccharides; Protein.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062016000100112
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Leaf anatomy and histochemistry of Macropeplus dentatus (Perkins) I. Santos & Peixoto and Macropeplus ligustrinus (Tul.) Perkins (Monimiaceae) 39
Costa,Fernanda Flores; Lima,Helena Regina Pinto; Cunha,Maura Da; Santos,Inês da Silva.
The Monimiaceae is represented in the Neotropical region by six genera. Macropeplus Perkins is a Brazilian genus comprising four species restricted to high-montane Atlantic Forest areas, which have not been properly examined from the anatomical point of view. This study characterizes the anatomical structure and histochemical aspects of the leaves of Macropeplus dentatus and M. ligustrinus, and adds to our taxonomic knowledge ofthe genus. The individuals studied were collected in the Serra dos Órgãos National Park and in the Caraça Natural Park in southeastern Brazil. Among the characteristics common to the two species, hypostomatic leaves, dorsiventral structure, paracytic stomata, a subepidermal layer, collateral vascular system in the form of an arc in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Atlantic Rain Forest; Histochemistry; Leaf anatomy; Macropeplus; Monimiaceae.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062010000300027
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Morphoanatomy of nectaries of Chamaecrista (L.) Moench sections Chamaecrista , Caliciopsis and Xerocalyx (Leguminosae: Caesalpinioideae) 39
Silva,Marinalva dos Santos; Coutinho,Ítalo Antônio Cotta; Araújo,Maicon Nascimento; Meira,Renata Maria Strozi Alves.
ABSTRACT Nectaries are specialized structures that secrete nectar. Several species of Chamaecrista possess nectaries on the petiole, which have been shown to vary widely in morphology and the chemical nature of their secretion. However, a comprehensive investigation of the nectaries of the clade formed by sect. Chamaecrista, Caliciopsis and Xerocalyx has yet to be performed. Our study aimed to confirm whether or not the leaf glands of species of this clade are in fact nectaries, determine the chemical nature of their secretion and expand the morphoanatomical database on leaf nectaries in Chamaecrista with the intention of contributing to the taxonomy and phylogeny of the genus. Samples from herbarium and field-collected material were subjected to standard...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Foliar glands; Histochemistry; Leaf glands; Secretory structures; Taxonomy.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000300445
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Does an urban environment affect leaf structure of Eugenia uniflora L. (Myrtaceae)? 39
Bezerra,Laís de Almeida; Callado,Cátia Henriques; Cunha,Maura Da.
ABSTRACT External factors can interfere with the structure and biological activity of plants. Nevertheless, the susceptibility of plants to specific environmental conditions varies, which raises many questions about the behavior of medicinal plants when grown in urban areas. This study aims to detect possible changes induced by exposure of Eugenia uniflora L. to an urban environment, with emphasis on variation in external and internal leaf structure and differences in the production of its main metabolites. We compared leaves of E. uniflora cultivated in forest and urban sites and analyzed them for structural plasticity and characteristics indicative of stress in the urban environment. The leaves of the urban site revealed necrosis and reddish spots,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Atmospheric pollution; Brazilian cherry; Environmental change; Histochemistry; Pitangueira; Urban environment.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062020000200266
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Extrafloral nectaries of annatto ( Bixa orellana L.): anatomy, nectar composition and activity during organ development 39
Miranda,Rafaela Marques de; Nery,Lays Araújo; Ventrella,Marília Contin.
ABSTRACT This study aimed to anatomically characterize the extrafloral nectaries (EFNs) of annatto (Bixa orellana) and determine the composition of its nectar in order to better understand their structure and function during organ development. Standard light and scanning electron microscopy techniques were used for anatomical analysis, and test-strips and a refractometer for determining nectar composition. Both receptacle and stem EFNs were found to possess similar anatomy and nectar composition, and to secrete from early to advanced developmental stages of the organs to which they are associated. EFNs consisting of uniseriate epidermis, nectariferous parenchyma and subnectariferous parenchyma were found located where vascular tissue is immersed. Some...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ants; Histochemistry; Nectar release; Secretory structure; Urucum.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000300468
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Leaf glands of Banisteriopsis muricata (Malpighiaceae): distribution, secretion composition, anatomy and relationship to visitors 39
Nery,Lays Araújo; Vieira,Milene Faria; Ventrella,Marília Contin.
ABSTRACT Leaf glands are common structures in Malpighiaceae and exhibit great morphological diversity, yet information on their anatomy, secretion and type of visitors remains scarce. The aim of this study was to describe the distribution, anatomical development and chemical and functional properties of leaf glands of Banisteriopsis muricata (Malpighiaceae). Leaves at different stages of development were collected and processed according to standard techniques for light and scanning electron microscopy. Secretion composition was determined by histochemical tests and test-strips, while gland funciton was determined by field observation of interactions with visitors. Leaf glands were located on the petiole and on the abaxial base of the leaf blade. The gland...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anatomy; Ants; Extrafloral nectaries (EFNs); Histochemistry; Leaf glands; Nectar; Ontogeny.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000300459
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Antiherbivory defense mechanisms along an environmental gradient in restinga 39
Silva,Maiara Matilde da; Boeger,Maria Regina Torres; Melo Júnior,João Carlos Ferreira de; Santos,Bruno Francisco Sant’Anna dos.
ABSTRACT Plant-herbivore interactions have directed the evolution of defense mechanisms in plants, which can vary intraspecifically according to environmental quality. This study evaluated variation in the production of antiherbivory defenses in plant species distributed along an environmental gradient of restinga. The study was performed in Parque Estadual Acaraí, state of Santa Catarina, Brazil. Three species of four plant formations with different abiotic conditions were selected for study. Specific leaf area; water, nitrogen and carbon content; and presence of phenols, tannins, lignin and alkaloids, were evaluated in leaves of ten plants per species per plant formation. The results indicated that Symphyopappus casarettoi possesses Low Nutritional...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coastal plain; Herbivore-plant interaction; Histochemistry; Parque Estadual Acaraí; Physical defense; Secondary metabolites.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000400583
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Nutritious tissue in petals of Annonaceae and its function in pollination by scarab beetles 39
Gottsberger,Gerhard; Webber,Antonio Carlos.
ABSTRACT The feeding of pollinating dynastid-scarab beetles on nutritious tissue of Annonaceae flowers results in macroscopically visible gnawing marks on petals. In the present paper, we present and discuss examples of such gnawing marks on Annonaceae from the Cerrado and the Amazon Forest in Brazil. The localization of gnawing marks on the petals and the histochemistry of the nutritious tissues are emphasized. In some species, nutritious tissue is apparently distributed among all petals, while in other species it is more or less diffusely localized. There are also cases in which nutritious tissue occurs only on clearly localized regions of the inner petals. Petals of selected Amazon species were stained, and studied by light and scanning electron...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cerrado and Amazon forest Annonaceae; Electron microscopy; Histochemistry; Lipids; Mucilage cells in petals; Starch; Tannins.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062018000200279
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A reduced, yet functional, nectary disk integrates a complex system of floral nectar secretion in the genus Zeyheria (Bignoniaceae) 39
Machado,Silvia Rodrigues; Souza,Camila Vaz de; Guimarães,and Elza.
ABSTRACT The genus Zeyheria (Bignoniaceae) comprises only two species, both of which have been described as possessing a reduced and non-functional nectary disk. Despite the importance of this evolutionary change in the floral nectary, these functional assumptions have been based on disk size and on the distribution, abundance and histochemistry of corolla-borne trichomes. By combining methods on light and electron microscopy, here we investigated the functionality of the reduced nectary disk and describe all of the tissues and structures of the nectar chamber in order to determine the sites of floral nectar secretion in both Zeyheria species. . Our data find the floral nectary traits of both species to be very similar, although differing in their cellular...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bignoniaceae; Floral nectary; Functional anatomy; Histochemistry; Ultrastructure.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000300344
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Histochemical changes during the ontogeny of malagueta and biquinho pepper seeds 1
Abud, Haynna Fernandes; Araújo, Eduardo Fontes; Araujo, Roberto Fontes; Picoli, Edgard Augusto de Toledo; Gallão, Maria Izabel.
This study aimed to evaluate the structural changes and reserve deposition of malagueta and biquinho pepper seeds and to determine their chemical composition during ontogeny. The fruits were collected 25, 40, 55, 70, 85, and 100 days after anthesis (DAA). After collection, the seeds were extracted and fixed in FAA 50 and then stored in 70% ethanol. Subsequently, the seeds were embedded in methacrylate (Historesin) and sectioned with a microtome. The sections were stained with Toluidine blue O for anatomical analysis and Xylidine Ponceau, Periodic acid Schiff (PAS) and Lugol’s iodine solution for histochemical analysis. Sections were made using a cryomicrotome for acid phloroglucinol staining and Sudan III tests. Progressive lignification of the seed coat...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Capsicum frutescens; C. chinense; Chemical composition; Histochemistry; Reserves..
Ano: 2017 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/32492
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Sublethal effect of concentrations of Metarhizium anisopliae (metsch.) sorok on the larval stage and immunologic characteristics of Diatraea flavipennella (box) (lepidoptera: crambidae) 42
GUIMARÃES,JENNIFER; MARQUES,EDMILSON J.; WANDERLEY-TEIXEIRA,VALÉRIA; ALBUQUERQUE,AURISTELA C. DE; PASSOS,ELIANA M. DOS; SILVA,CÍNTHIA C.M.; TEIXEIRA,ÁLVARO A.C..
This work aimed to evaluate the effects of Metarhizium anisopliae on Diatraea flavipennella and investigate their immune response. Was observed the effect of M. anisopliae against larvae of D. flavipennella sprayed at concentrations of 103, 104, 105 conidia / mL, in which showed differences relative the larval period, extending up to 72.0 days in treatment and 25.0 days in the control. The results for hemocytes revealed that the most frequent cells when sprayed at the concentrations of 103, 105, 107 conidia / mL were the prohemocytes, spherulocytes, plasmatocytes and granulocytes in relation to adipohemocytes and oenocytoids. The level of nitric oxide was different between the control and the concentration 107 spores / mL (24), while the activity of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Entomopathogen; Hemocytes; Histochemistry; Nitric oxide; Phenoloxidases; Sugarcane borer.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000401973
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All green, but equal? Morphological traits and ecological implications on spores of three species of mosses in the Brazilian Atlantic forest 42
MACIEL-SILVA,ADAÍSES S.; SILVA,FLÁVIA C.L. DA; VÁLIO,IVANY F.M..
Spores of the tropical mosses Pyrrhobryum spiniforme, Neckeropsis undulata and N. disticha were characterized regarding size, number per capsule and viability. Chemical substances were analyzed for P. spiniforme and N. undulata spores. Length of sporophyte seta (spore dispersal ability) was analyzed for P. spiniforme. Four to six colonies per species in each site (lowland and highland areas of an Atlantic Forest; Serra do Mar State Park, Brazil) were visited for the collection of capsules (2008 – 2009). Neckeropsis undulata in the highland area produced the largest spores (ca. 19 µm) with the highest viability. The smallest spores were found in N. disticha in the lowland (ca. 13 µm). Pyrrhobryum spiniforme produced more spores per capsule in the highland...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bryophytes; Histochemistry; Life histories; Spore size; Spore production; Tropical rain forest.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000301249
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Anatomical, phytochemical and histochemical study of Solidago chilensis Meyen 42
SOUZA,DOUGLAS M.F. DE; SÁ,RAFAELA D.; ARAÚJO,EVANI L.; RANDAU,KARINA P..
ABSTRACT Solidago chilensis Meyen, belonging to the family Asteraceae, is a plant native to South America and the only representative of the genus in Brazil. This species is popularly known as “arnica” and is used to treat bruises, muscle pain and inflammation. The objective of this work was to contribute to the pharmacognostic standardization of S. chilensis. Cross-sections were obtained, by freehand, for microscopic analysis of root, stem and leaf; for these parts of the plant maceration was also performed according to the method of Jeffrey. For the leaf were still made paradermal sections, scanning electron microscopy analysis, phytochemical and histochemical tests. Thus, it was determined anatomical features useful for diagnosis of the species which,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anatomy; Arnica; Asteraceae; Histochemistry; Phytochemistry; Solidago chilensis.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000502107
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Trichomes and chemical composition of the volatile oil of Trichogonia cinerea (Gardner) R. M. King & H. Rob. (Eupatorieae, Asteraceae) 42
FERNANDES,YANNE S.; TRINDADE,LUMA M.P.; REZENDE,MARIA HELENA; PAULA,JOSÉ R.; GONÇALVES,LETÍCIA A..
Trichogonia cinerea is endemic to Brazil and occurs in areas of cerrado and campo rupestre. In this study, we characterized the glandular and non-glandular trichomes on the aerial parts of this species, determined the principal events in the development of the former, and identified the main constituents of the volatile oil produced in its aerial organs. Fully expanded leaves, internodes, florets, involucral bracts, and stem apices were used for the characterization of trichomes. Leaves, internodes, florets, and involucral bracts were examined by light microscopy and scanning electron microscopy, whereas stem apices were examined only by light microscopy. Branches in the reproductive phase were used for the extraction and determination of the composition...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Volatile oil; Histochemistry; Ontogeny; Secretory structure.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000100309
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Morphological and histochemical study of the masseter muscle after occlusal alteration 47
Mardegan Issa,João Paulo; Tiossi,Rodrigo; Mizusaki Iyomasa,Mamie.
The aim of this study was to evaluate alterations in the masseter muscle of 30 male guinea-pigs submitted to occlusal alteration. The animals were divided into 2 equal groups, the control group (C) only submitted to surgical stress, and the occlusal altered group (T) submitted to teeth extraction. Each group was subdivided into 3 groups, with 5 animals, for the following studies: macroscopy and vessels distribution, light microscopy and histochemical analysis, with animals' perfusion 2 months after surgery. Data were submitted to statistical analysis. Macroscopically, an anteroposterior orientation of the muscular fibers was found and statistical difference between C and T groups in width (p<0.05). Microscopically, C and T groups showed polygonal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Masseter muscle; Morphology; Histology; Histochemistry.
Ano: 2007 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000300003
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Morphological and histochemical analysis of the antennal gland of Macrobrachium Potiuna (Muller, 1880) (Crustácea, Decapoda, Palaemonidae) 52
Moraes,Neci; Scaff,Rejane Cirra.
The objective of our study was to describe by histologic methods the structural organization of the antennal gland of M. potiuna as well as the location through histochemical methods of the neutral and sulfated polissacharides that exist in these regions. This study showed that the gland is composed of the a non-secretive tubular portion and a secretive portion which is formed by a cubic simple epithelium, sub-epithelium layer and cellular chains
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antennal gland; Macrobrachium potiuna; Morphology; Histochemistry.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000300005
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Storage sites in seeds of Caesalpinia echinata and C. ferrea (Leguminosae) with considerations on nutrients flow 52
Teixeira,Simone de Pádua; Machado,Sílvia Rodrigues.
The seeds of Caesalpinia echinata and C. ferrea behaved as typical endospermic seeds, despite their different morphological classification (exendospermic seeds were described for C. echinata and endospermic seeds for C. ferrea). Then, the aim of this work was to compare, under ultrastructural and histochemical terms, the nature of the storage substances and their accumulation sites, as well as the nutrient flow in seeds of these species. Cotyledons in C. echinata accumulate carbohydrates, lipids and proteins, which are mobilized from the outer to the inner parts as revealed by the position of plasmodesmata. Endosperm in C. ferrea accumulates carbohydrates and in C. echinata accumulates substances during the initial embryogenic phases. Such tissue develops...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cotyledon; Embryo; Endosperm; Histochemistry; Reserve substances; Ultrastructure.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100016
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Structural, hystochemical and cytochemical characteristics of the stigma and style in Passiflora edulis f. flavicarpa (Passifloraceae) 52
Souza,Margarete Magalhães; Pereira,Telma Nair Santana; Dias,Angelo José Burla; Ribeiro,Beatriz Ferreira; Viana,Alexandre Pio.
Studies were carried out on anatomic aspects of the stigma and style of yellow passionfruit. The stigmatic style consisted of papillae, many layers of sub-epidermal cells and a central transmitting tissue. The stigma was of dry, unbranched, multicellular multiseriate papillate type and the cells were highly vacuolated with walls that were relatively thin consisting of compactly arranged fibrils. The style was solid with a central core of transmitting tissue that had intercellular spaces containing a matrix that included polysaccharidic and lipidic substances. During development, cells of the transmitting tissue were arranged compactly while the mature pistil showed cells with a little more loosely arranged intercellular spaces. Both the mature stigma and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Yellow passionfruit; Anatomy; Stigma and style; Histochemistry; Cytochemistry.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100011
Registros recuperados: 58
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