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| Santana,Priscila Bessa; Ghilardi Junior,Rubens; Alves,Claudio Nahum; Silva,Jeronimo Lameira; McCulloch,John Anthony; Schneider,Maria Paula Cruz; Silva,Artur da Costa da. |
| Methanogenic archaeans are organisms of considerable ecological and biotechnological interest that produce methane through a restricted metabolic pathway, which culminates in the reaction catalyzed by the Methyl-coenzyme M reductase (Mcr) enzyme, and results in the release of methane. Using a metagenomic approach, the gene of the a subunit of mcr (mcrα) was isolated from sediment sample from an anoxic zone, rich in decomposing organic material, obtained from the Tucuruí hydroelectric dam reservoir in eastern Brazilian Amazonia. The partial nucleotide sequences obtained were 83 to 95% similar to those available in databases, indicating a low diversity of archaeans in the reservoir. Two orders were identified -the Methanomicrobiales, and a unique Operational... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amazonian; Archaea; Diversity; Methyl-coenzyme M reductase; Mcrα; Homology modeling. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100018 |
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| Flores-Sanchez,Isvett J.; Linthorst,Huub J.M.; Verpoorte,Robert. |
| Cannabinoids, flavonoids, and stilbenoids have been identified in the annual dioecious plant Cannabis sativa L. Of these, the cannabinoids are the best known group of this plant's natural products. Polyketide synthases (PKSs) are responsible for the biosynthesis of diverse secondary metabolites, including flavonoids and stilbenoids. Biosynthetically, the cannabinoids are polyketide substituted with terpenoid moiety. Using an RT-PCR homology search, PKS cDNAs were isolated from cannabis plants. The deduced amino acid sequences showed 51%-73% identity to other CHS/STS type sequences of the PKS family. Further, phylogenetic analysis revealed that these PKS cDNAs grouped with other non-chalcone-producing PKSs. Homology modeling analysis of these cannabis PKSs... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cannabis sativa; Homology modeling; Polyketide synthases; RT-PCR. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400017 |
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| Rádis-Baptista,G.; Moreno,F. B. M. B.; Nogueira,L. L.; Martins,A. M. C.; Toyama,D. O.; Toyama,M. H.; Azevedo Jr,W. F.; Cavada,B. S.; Yamane,T.. |
| Snake venom (sv) C-type lectins encompass a group of hemorrhagic toxins, which are able to interfere with hemostasis. They share significant similarity in their primary structures with C-type lectins of other animals, and also present a conserved carbohydrate recognition domain (CRD). A very well studied sv C-type lectin is the heterodimeric toxin, convulxin (CVX), from the venoms of South American rattlesnakes, Crotalus durissus terrificus and C. d. cascavella. It consists of two subunits, alfa (CVXalpha , 13.9 kDa) and beta (CVXbeta , 12.6 kDa), joined by inter and intra-chain disulfide bounds, and is arranged in a tetrameric alpha4beta4 conformation. Convulxin is able to activate platelet and induce their aggregation by acting via p62/GPVI collagen... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Crotalus durisssus venom; Snake venom C-type lectin; Homology modeling; Platelet aggregation; Antimicrobial activity. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400013 |
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| Menezes,Alana Manoela Fraga; Cavalcanti Junior,Edilton de Albuquerque; Martins,Luiza Suely Semen; Moraes Filho,Rômulo Maciel de. |
| Abstract Root-knot nematodes are a group of endoparasites species that induce the formation of giant cells in the hosts, by which they guarantee their feeding and development. Meloidogyne species infect over 2000 plant species, and are highly destructive, causing damage to many crops around the world. M. enterolobii is considered the most aggressive species in tropical regions, such as Africa and South America. Phytonematodes are able to penetrate and migrate within plant tissues, establishing a sophisticated interaction with their hosts through parasitism factors, which include a series of cell wall degradation enzymes and plant cell modification. Among the parasitism factors documented in the M. enterolobii species, cellulose binding protein (CBP), a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bioinformatics; Phytonematodes; Parasitism Factors; Cell wall degrading enzymes; Homology modeling; Protein function prediction. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100201 |
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| Teng,Feixiang; Sun,Jinxia; Yu,Lili; Li,Qisong; Cui,Yubao. |
| Dermatophagoides farinae (Der f), one of the main species of house dust mites, produces more than 30 allergens. A recently identified allergen belonging to the alpha-tubulin protein family, Der f 33, has not been characterized in detail. In this study, we used bioinformatics tools to construct the secondary and tertiary structures and predict the B and T cell epitopes of Der f 33. First, protein attribution, protein patterns, and physicochemical properties were predicted. Then, a reasonable tertiary structure was constructed by homology modeling. In addition, six B cell epitopes (amino acid positions 34–45, 63–67, 103–108, 224–230, 308–316, and 365–377) and four T cell epitopes (positions 178–186, 241–249, 335–343, and 402–410) were predicted. These... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Der f 33; Homology modeling; B-cell epitope; T-cell epitope; Prediction. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500601 |
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