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| Registros recuperados: 29 | |
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| Heng,Boon Chin; Vinoth,Kumar Jayaseelan; Lu,Kai; Deng,Xuliang; Ge,Zigang; Bay,Boon Huat; Cao,Tong. |
| We investigated the effects of prolonged heat shock treatment on human embryonic stem cell (hESC) viability. The hESC viability steadily declined with longer exposure to heat shock treatment (43ºC). After 4 h of exposure to heat shock at 43ºC, only 56.2 ± 1.5% of cells were viable. Viability subsequently declined to 37.0 ± 3.3% and 3.5 ± 0.7% after 8 h and 16 h, respectively of heat shock treatment at 43ºC. Transmission electron micrographs showed that the morphology of the dead/dying cells after heat shock treatment was characteristic of cellular necrosis with an uncondensed chromatin and a non-intact plasma membrane. This was further confirmed by flow cytometry analysis which showed that the DNA of the dead/ dying cells was still mostly intact, unlike... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Embryonic; Heat shock; Human; Necrosis; Stem cells. |
| Ano: 2007 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000300007 |
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| Wang,Jiangtao; He,Qingliu; Yan,Xingyu; Cai,Youmei; Chen,Junyi. |
| BACKGROUND: Nitric oxide (NO) has been shown to be important in sperm function, and the concentration of NO appears to determine these effects. Studies have demonstrated both positive and negative effects of NO on sperm function, but have not been able to provide a clear link between NO concentration and the extent of exposure to NO. To study the relationship between nitric oxide and sperm capacitationin vitro, and to provide a theoretical basis for the use of NO-related preparations in improving sperm motility for in vitro fertilization, we investigated the effects of NO concentration and time duration at these concentrations on in vitro sperm capacitation in both normal and abnormal sperm groups. We manipulated NO concentrations and the time duration of... |
| Tipo: Journal article |
Palavras-chave: Nitric oxide; Sperm capacitation; Sperm motility; Time; Human. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100044 |
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| Corrêa,F.G.; Pontes,C.L.S.; Verzola,R.M.M.; Mateos,J.C.P.; Velho,P.E.N.F.; Schijman,A.G.; Selistre-de-Araujo,H.S.. |
| Infection with Bartonella spp may cause cardiac arrhythmias, myocarditis and endocarditis in humans. The aim of the present study was to evaluate a possible association between Bartonella spp bacteremia and endocarditis, arrhythmia and Chagas cardiomyopathy in patients from Brazil and Argentina. We screened for the presence of bacterial 16S rRNA in human blood by PCR using oligonucleotides to amplify a 185-bp bacterial DNA fragment. Blood samples were taken from four groups of subjects in Brazil and Argentina: i) control patients without clinical disease, ii) patients with negative blood-culture endocarditis, iii) patients with arrhythmias, and iv) patients with chronic Chagas cardiomyopathy. PCR products were analyzed on 1.5% agarose gel to visualize the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Human; PCR; Bartonella spp; Chagas disease; Endocarditis; Arrhythmia. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000700012 |
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| Agudelo-Flórez,P.; López,J.A.; Redher,J.; Carneiro-Sampaio,M.M.S.; Costa-Carvalho,B.T.; Grumach,A.S.; Condino-Neto,A.. |
| Chronic granulomatous disease (CGD) is an inherited disorder of the innate immune system characterized by a defective oxidative burst of phagocytes and subsequent impairment of their microbicidal activity. Mutations in one of the NADPH-oxidase components affect gene expression or function of this system, leading to the phenotype of CGD. Defects in gp91-phox lead to X-linked CGD, responsible for approximately 70% of CGD cases. Investigation of the highly heterogeneous genotype of CGD patients includes mutation analysis, Northern blot or Western blot assays according to the particular case. The aim of the present study was to use reverse transcription (RT)-PCR for the analysis of molecular defects responsible for X-linked CGD in eight Brazilian patients and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Superoxide; Phagocytes; Primary immunodeficiency; Respiratory burst; Neutrophils; Human. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000500001 |
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| Qiao,Suchi; Liu,Chang; Xu,Weijie; AZhaTi,WuBuLi; Li,Cheng; Wang,Zhiwei. |
| It is increasingly evident that the microenvironment of bone can influence cancer phenotype in many ways that favor growth in bone. CD147, a transmembrane protein of the immunoglobulin (Ig) superfamily, was identified independently in different species and has many designations across different species. However, expression levels of CD147 mRNA in bone cancer have not been described. In this study, we have used real-time fluorescence quantification (RT-PCR) to demonstrate CD147 expression in malignant bone cancer and benign bone tumor tissues. The results suggested that the expression of CD147 gene was significantly up-regulated in malignant bone cancer. Moreover, we found that over-expressed RANKL progressively enhanced osteoclast formation up to 48 h,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bone cancer; RT-PCR; Western blot; RANKL; Human. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000900608 |
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| Hidalgo,M.P.; Camozzato,A.; Cardoso,L.; Preussler,C.; Nunes,C.E.; Tavares,R.; Posser,M.S.; Chaves,M.L.F.. |
| The Horne-Östberg questionnaire partly covers some factors that may be important determinants of peak time and characterize patterns of behavior. We conducted a study for the evaluation of self-reported behavioral states (hunger sensation, availability for study, physical exercise, solving daily problems, and time preferences) as expressions of underlying cyclic activity. Three hundred and eighteen community subjects without history of medical, psychiatric, or sleep disorders were evaluated in a cross-sectional design. A self-report about daily highest level of activity was used to categorize individuals into morning, evening, and indifferently active. Time-related behavioral states were evaluated with 23 visual analog questions. The responses to most... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Chronobiology; Morningness; Eveningness; Circadian rhythm; Behavior; Human. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000700012 |
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| Gonçalves,Daniela Dib; Benitez,Aline; Lopes-Mori,Fabiana Maria Ruiz; Alves,Lucimara Aparecida; Freire,Roberta Lemos; Navarro,Italmar Teodorico; Santana,Maria Aparecida Zanella; Santos,Luís Roberto Alves dos; Carreira,Teresa; Vieira,Maria Luísa; Freitas,Julio Cesar de. |
| The aim of this study was to conduct a serological survey for Lyme diseases, brucellosis, leptospirosis and toxoplasmosis and identify the risk variables related to these zoonoses in humans living in the rural area of Jataizinho, state of Parana, Brazil. A total of 63 rural properties were surveyed. Additionally, 207 serum samples collected from these rural area inhabitants were tested for indirect immunofluorescence (IFI) and western blots (WB) were performed to detect Borrelia burgdorferi (sensu lato); a tamponated acidified antigen test (AAT) and 2-mercaptoethanol (2-ME) were used to detect antibodies of Brucella abortus; the microscopic agglutination test (MAT) was carried out to detect antibodies anti-Leptospira spp. and IFI was used to find... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lyme disease; Leptospirosis; Brucellosis; Toxoplasmosis; Human; Antibody. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100018 |
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| Aguiar,Daniel M.; Cavalcante,Guacyara T.; Camargo,Luis M. A.; Labruna,Marcelo B.; Vasconcellos,Silvio A.; Souza,Gisele O.; Gennari,Solange M.. |
| Sera from 276 humans living in 71 farms located in Monte Negro Municipality, RO., Western Amazon, Brazil were examined for anti-Leptospira spp antibodies by Microscopic Agglutination Test and for anti-Brucella spp antibodies by Tube Agglutination Test. Leptospira spp antibodies were detected in 28 (10.2%) of them with at least one positive case in 23 farms (32.4%). The most frequent leptospira serovars were Patoc (46.7%), Autumnalis (10.0%) and Shermani (10.0%). The proportion of positive males (14.5%) were higher than females (5.0%; P <0.05) and the contact with river water presented association with Leptospira spp infection (OR: 27; P=0.01). A total of 04 (1.4%) humans reacted against Brucella antigens; three farms (4.2%) presented at least one... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Human; Leptospirosis; Brucellosis; Amazon; Brazil. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100019 |
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| Amaro,André L. N.; Yanagi Junior,Tadayuki; Yanagi,Sílvia de N. M.; Ferraz,Gabriel A. E S.; Campos,Alessandro T.. |
| Abstract The aim of the present research was to propose a bioclimatic mapping to classify the thermal comfort and discomfort of rural workers within the state of Minas Gerais, considering historical and future scenarios. Monthly historical series (1976-2014) of minimum, mean and maximum temperature-humidity index (THI), determined through the values of air temperature (minimum, mean and maximum) and relative humidity from 48 weather stations located in the state of Minas Gerais were used to analyze the trends through Mann-Kendall and linear regression assays. The bioclimatic mapping of human comfort, obtained via geostatistical analysis, was developed as a function of the minimum, medium and maximum THI for the historical period (1976-2014) and future... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Human; Biometeorology; Human thermal comfort; THI; Time series. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69162018000200173 |
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| Pearson,Peter L.; Madan,Kamlesh. |
| Abstract Polyploidy does not usually occur in germinal cells of mammals and other higher vertebrates. We describe a unique example of mosaic autotetraploidy in the meiosis of a human male. Although the original observations were made in the late 1960s, we did not publish them at that time, because we expected to detect further examples that could be described together. However, this did not occur and we have now decided to make the observations available to demonstrate that polyploidy in mammalian male meiosis can arise at a higher frequency than expected by random polyploidization of individual meiotic cells, by either DNA duplication or cell fusion prior to synapsis. This is the first description of a population of primary spermatocytes exhibiting... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Polyploidy; Meiosis; Human; Male. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300410 |
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| Furet, Jean-pierre; Firmesse, Olivier; Gourmelon, Michele; Bridonneau, Chantal; Tap, Julien; Mondot, Stanislas; Dore, Joel; Corthier, Gerard. |
| Pollution of the environment by human and animal faecal pollution affects the safety of shellfish, drinking water and recreational beaches. To pinpoint the origin of contaminations, it is essential to define the differences between human microbiota and that of farm animals. A strategy based on real-time quantitative PCR (qPCR) assays was therefore developed and applied to compare the composition of intestinal microbiota of these two groups. Primers were designed to quantify the 16S rRNA gene from dominant and subdominant bacterial groups. TaqMan((R)) probes were defined for the qPCR technique used for dominant microbiota. Human faecal microbiota was compared with that of farm animals using faecal samples collected from rabbits, goats, horses, pigs, sheep... |
| Tipo: Text |
Palavras-chave: Quantitative PCR; Faecal microbiota; Human; Farm animals. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00000/11071/11297.pdf |
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| Cachot, Jerome; Bultelle, Florence; Drouot, Laurent; Galgani, Francois; Frebourg, Thierry; Leboulenger, François; Danger, Jean-michel. |
| Screening of a flounder ovary cDNA library with a rainbow trout p53 probe led to the isolation of a p53-unrelated cDNA encoding an unknown 161 amino acid protein. In view of its apparent molecular weight and yet unknown function, the deduced protein was named Xp18. Corresponding orthologous cDNAs or expressed sequence tags have been identified in several species including human, rodents, bovine, chicken and zebrafish and a related cDNA has also been isolated in the fruit fly. Deduced amino acid sequences appeared to be extremely well conserved throughout vertebrate evolution. Structure predictions suggested that Xp 18 may correspond to an integral protein comprising four transmembrane domains. The charged C-termini of all known vertebrate Xp18-like... |
| Tipo: Text |
Palavras-chave: X chromosome; Brain; Ovary; Human; Teleostean; Platichthys flesus. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/publication-756.pdf |
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| Pokhariyal,Ganesh; Hassanali,Jameela. |
| The variations in morphometric parameter of mammalian brains may be influenced by process of functional complexity, evolution and adaptation. Comparative analysis of linear measurements of cerebrum in the human and baboon has shown morphometric differences. In the present study linear measurements from human and baboon cerebrum (n=10 each) were used to predict various values for human and baboon brain and body parameters through multiple regression models. The average brain weights were found to be 2.08% and 0.84% of the body weights for humans and baboons respectively. The elasticity of regression models revealed that unit percentage increase in Occipital-Frontal (OF) distance would increase the human brain weight by 66.19%, while the baboon brain weight... |
| Tipo: Journal article |
Palavras-chave: Morphometric parameters; Human; Baboon; Brains; Regression and simulation models. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000300052 |
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| Pretorius,E; Vieira,W. A; Oberholzer,H. M; Auer,R. E. J. |
| The purpose of the present study was to compare the platelet and fibrin network ultrastructure of humans to eight different animal species in order to determine the differences between human and animal platelet and fibrin morphology, and to determine whether the animals studied differ in their platelet and fibrin morphology, and whether these differences can be observed by scanning electron microscopy. Platelets and fibrin networks play an important role both in the coagulation process as well as physiologically in allergic processes and immunological mechanisms. The thickness of human fibrin networks were compared to mouse (Mus musculus), equine (Equus caballus), vervet monkey (Chlorocebus aethiops previously Cercopithecus aethiops), oryx (Oryx gazella),... |
| Tipo: Journal article |
Palavras-chave: Scanning electron microscopy; Platelets; Fibrin networks; Human; Animals. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000100013 |
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| Registros recuperados: 29 | |
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