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Obtaining interspecific hybrids, and molecular analysis by microsatellite markers in grapevine PAB
Schuck,Mariane Ruzza; Biasi,Luiz Antonio; Mariano,Ada Michele; Lipski,Bernardo; Riaz,Summaira; Walker,Michael Andrew.
The objective of this work was to assess the potential of interspecific hybridization of Vitis labruscana and Muscadinia rotundifolia by using artificial cross-pollinations. Microsatellite markers were used to confirm interspecific hybridizations and the identity of the parental genotypes. In crosses in which M. rotundifolia was used as the female parent, no true hybrids were obtained. In the reciprocal crosses, 114 seedlings were identified as true V. labruscana x M. rotundifolia hybrids. Self pollination occurred in direct and in reciprocal crosses. The crossings between 'Bordo' x 'Carlos', 'Magnolia', 'Regale' and' Roanoke', and between' Isabel' x 'Bountiful', 'Carlos', 'Magnolia', 'Regale' and 'Roanoke' were confirmed. The 15 markers evaluated showed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Muscadinia rotundifolia; Vitis labruscana; Hybrid identification; Interspecific crosses; SSRs.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011001100009
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Genetic similarity in a hybrid population of 'Montenegrina' and 'King' mandarins PAB
Brugnara,Eduardo Cesar; Bastianel,Marinês; Weiler,Roberto Luis; Schwarz,Sergio Francisco.
The objective of this work was to confirm the hybrids obtained in plants originated from the crossing between the mandarins Citrus deliciosa 'Montenegrina' and C. nobilis 'King', and to estimate the genetic similarity among hybrids, and between each hybrid and its parents. Twenty‑three pairs of microsatellite primers were tested. Fourteen of these pairs showed polymorphic bands between parents. Primers CCSM 129 and CCSME 52 were sufficient to identify the 12 nucellar clones observed in the studied population. Genetic similarity analysis of the population (hybrids and parents) showed 0.56 average similarity. Besides the 12 clones of 'Montenegrina' identified, 25 hybrids were found of which D18, C32, D06, C05 and D09 are the more similar to 'Montenegrina'.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus deliciosa; Citrus nobilis; Hybrid identification; Microsatellite markers.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012000800010
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