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Effects of H2O2, Fe2+ and Fe3+ on curcumin-induced chromosomal aberrations in CHO cells Genet. Mol. Biol.
Antunes,Lusânia Maria Greggi; Araújo,Maria Cristina Paiva; Dias,Francisca da Luz; Takahashi,Catarina Satie.
The effects of H2O2, Fe2+ and Fe3+ on curcumin-induced clastogenicity were evaluated in CHO cells. Curcumin combined with H2O2 did not increase the chromosomal aberrations more than expected based on a simple additive effect. In contrast, the combination of curcumin-Fe significantly decreased the total number of chromosomal aberrations and the number of abnormal metaphases. The clastogenicity of curcumin may be related to its pro-oxidant properties and its ability to generate free radicals.
Tipo: Info:eu-repo/semantics/article Palavras-chave: CHO; Chromosomal aberrations; Curcumin; Hydrogen peroxide; Mutagenesis.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100028
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Several pathways of hydrogen peroxide action that damage the E. coli genome Genet. Mol. Biol.
Asad,Nasser Ribeiro; Asad,Lidia Maria Buarque Oliveira; Almeida,Carlos Eduardo Bonacossa de; Felzenszwalb,Israel; Cabral-Neto,Januário Bispo; Leitão,Alvaro Costa.
Hydrogen peroxide is an important reactive oxygen species (ROS) that arises either during the aerobic respiration process or as a by-product of water radiolysis after exposure to ionizing radiation. The reaction of hydrogen peroxide with transition metals imposes on cells an oxidative stress condition that can result in damage to cell components such as proteins, lipids and principally to DNA, leading to mutagenesis and cell death. Escherichia coli cells are able to deal with these adverse events via DNA repair mechanisms, which enable them to recover their genome integrity. These include base excision repair (BER), nucleotide excision repair (NER) and recombinational repair. Other important defense mechanisms present in Escherichia coli are OxyR and SosRS...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hydrogen peroxide; Cross-adaptive response; Lethal synergism; Copper and iron.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200026
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Réaction de réduction de l'oxygène sur les aciers inoxydables en eau de mer naturelle. Influence du biofilm sur les processus de corrosion ArchiMer
Le Bozec, Nathalie.
iofilm development on stainless steels immersed in natural seawater can have prejudicial consequences on the resistance of these materials to corrosion . The goal of the present study was to get more precise information on the corrosion processes, and especially on the oxygen reduction reaction. As the reaction is linked to the stainless steel surface state, the characterisation of the oxides films (composition, structure, thickness...) is essential to understand the mechanisms and the oxygen reduction kinetic. The first aim of the study has been to correlate the oxygen reduction processes with the characteristics of the oxides layer as a function of the alloy surface treatment (mechanical polishing, electrochemical passivation and prereduction, chemical...
Tipo: Text Palavras-chave: Hydrogen peroxide; Oxides layer; Biofilm; Oxygen reduction; Corrosion; Seawater; Stainless steels; Peroxyde d'hydrogène; Films d'oxydes; Biofilm; Réduction de l'oxygène; Corrosion; Eau de mer; Aciers inoxydables.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2004/these-223.pdf
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The decomposition of hydrogen peroxide by marine phytoplankton ArchiMer
Wong, Gtf; Dunstan, Wm; Kim, Db.
All nine species of marine phytoplankton tested (a cyanobacterium: Synechococcus sp., three diatoms: Chaetoceros simplex, Thalassiosira oceanica and Skeletonema costatum, two prymnesiophytes: Pleurochrysis carterae and Isochrysis galbana, a prasinophyte: Tetraselmis sp., a green alga: Dunaliella tertiolecta, and a dinoflagellate: Amphidinium carterae) were able to decompose hydrogen peroxide in the dark. Since these phytoplankton species can be found widely in a variety of marine sub-environments, this indicates that the dark decomposition of hydrogen peroxide by phytoplankton is a general phenomenon in the oceans. The decomposition rates were first order with respect to the concentration of hydrogen peroxide and biomass. The second-order rate constants...
Tipo: Text Palavras-chave: Phytoplancton marin; Peroxyde d'hydrogène; Oxydo-réduction; Marine phytoplankton; Hydrogen peroxide; Oxidation-reduction.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/00322/43276/43007.pdf
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Development of a comet-FISH assay for the detection of DNA damage in hemocytes of Crassostrea gigas ArchiMer
Perez Garcia, Maria Concepcion; Rouxel, Julien; Akcha, Farida.
In this work, the DNA-damaging effect of hydrogen peroxide on the structural integrity of nucleolar organizer regions (NORs) was studied for the first time by comet-FISH in the Pacific oyster Crassostrea gigas. Global DNA damage was assessed in hemocytes using an alkaline version of the comet assay. Next, NOR sensitivity was analysed by mapping major rDNA repeat unit by fluorescence in situ hybridisation (FISH) on the same comet slides. Exposure of hemocytes to 100 μM of hydrogen peroxide induced a significant increase in both DNA damage and number of FISH-signals of major ribosomal genes versus the control. Moreover, a significant positive correlation was shown between DNA damage as measured by the comet assay (percentage of DNA in comet tail) and the...
Tipo: Text Palavras-chave: Crassostrea gigas; Hydrogen peroxide; Comet assay; Comet-FISH; Genotoxicity.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00250/36157/34712.pdf
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The ABTS-HRP System as an Alternative Method to RRDE for the Determination of the Selectivity of the Oxygen Reduction Reaction ArchiMer
Cheng, Xi; Challier, Lylian; Etcheberry, Arnaud; Noel, Vincent; Perez, Henri.
A 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS)-H2O2-peroxidase system was involved to study the oxygen reduction reaction (ORR) selectivity in acid medium. The average number of electron transferred per oxygen molecule (n) was calculated by measuring the quantity of H2O2 produced during the oxygen reduction reaction, taking advantage of the high activity of peroxidase enzyme coupled with the ABTS chromogen. The method was validated using electrode materials for which the electron pathway is established. It is simple, accurate and can be used on various kind of electrode structure.
Tipo: Text Palavras-chave: ORR; Selectivity; Hydrogen peroxide; Porous electrodes.
Ano: 2012 URL: https://archimer.ifremer.fr/doc/00692/80365/83471.pdf
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New Insight into Antimicrobial Compounds from Food and Marine-Sourced Carnobacterium Species through Phenotype and Genome Analyses ArchiMer
Begrem, Simon; Ivaniuk, Flora; Gigout Chevalier, Frederique; Kolypczuk, Laetitia; Bonnetot, Sandrine; Leroi, Francoise; Grovel, Olivier; Delbarre-ladrat, Christine; Passerini, Delphine.
Carnobacterium maltaromaticum and Carnobacterium divergens, isolated from food products, are lactic acid bacteria known to produce active and efficient bacteriocins. Other species, particularly those originating from marine sources, are less studied. The aim of the study is to select promising strains with antimicrobial potential by combining genomic and phenotypic approaches on large datasets comprising 12 Carnobacterium species. The biosynthetic gene cluster (BGCs) diversity of 39 publicly available Carnobacterium spp. genomes revealed 67 BGCs, distributed according to the species and ecological niches. From zero to six BGCs were predicted per strain and classified into four classes: terpene, NRPS (non-ribosomal peptide synthetase), NRPS-PKS (hybrid...
Tipo: Text Palavras-chave: Lactic acid bacteria; Antimicrobial activity; Carnobacteriumspp; Hydrogen peroxide; Bacteriocin; RiPP; NRPS; Terpene; Natural product; Genome mining.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00641/75335/75999.pdf
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Immunomodulatory action of propolis on macrophage activation J. Venom. Anim. Toxins
ORSI,R.O.; FUNARI,S.R.C.; SOARES,A.M.V.C.; CALVI,S.A.; OLIVEIRA,S.L.; SFORCIN,J.M.; BANKOVA,V..
Propolis has been the subject of several recent studies, with the aim of elucidating its biological and pharmacological properties. Propolis has a well-known antimicrobial activity as well as antioxidant, antitumoral, antiinflammatory, and regenerative properties, but literature about its effects on the immune response is scarce. The goal of this work was to evaluate the propolis effect on macrophage activation by oxygen (H2O2) and nitrogen (NO) metabolite determination. Propolis was produced by africanized honeybees and hydroalcoholic solutions were prepared at different concentrations. Peritoneal macrophages were obtained from male BALB/c mice and culture cells were stimulated in vitro with propolis or interferon-gamma (IFN-gamma). In the in vivo assay,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Propolis; Macrophages; Hydrogen peroxide; Nitric oxide.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302000000200006
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Physical constraints of bath treatments of Atlantic salmon (Salmo salar) with a sea lice burden (Copepoda: Caligidae) Naturalis
Treasurer, James W.; Grant, Andrew; Davis, Paul J..
Licensed medicines available in the U.K. for treating Atlantic salmon infested with sea lice, dichlorvos, azamethiphos, and hydrogen peroxide, can only be administered by bath application. Adverse reactions have been reported to bath treatments including mortalities, inappetance, reduction in growth and reduced louse sensitivity to dichlorvos. The physical constraints of bath treatments are examined and improvements recommended. Oxygen saturation was adequate during treatments but declined rapidly when the tarpaulin was removed. A chemical marker dispersed uniformally both horizontally and vertically in a cage within 5 mins of dispensing indicating dispersal of a medicine is rapid and adequate during treatment. The range in enclosed volumes in 86...
Tipo: Article / Letter to the editor Palavras-chave: Sea lice; Atlantic salmon; Salar; Bath application; Dichlorvos; Hydrogen peroxide.
Ano: 2000 URL: http://www.repository.naturalis.nl/record/534338
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Evaluation of pregerminative treatments in gulupa seeds (P. edulis f. edulis Sims). Rev. Bras. Frutic.
Camargo,María Antonia Martínez; Coronado,Ana Cruz Morillo; Mora,Edilberto Cepeda; Fernández,Sandra Yaneth Mesa.
Abstract The genus Passiflora, commonly known as fruits of passion, includes numerous species, including gulupa (Passiflora edulis f. edulis), of nutracetic and economic importance, both for consumption in nature and for its agro-industrial applicability. This research was developed to evaluate the effects of various pre-germinative treatments on the germination of gulupa seeds from the municipality of Ramiriquí (Boyacá), such as: exposure to hydrogen peroxide (H2O2), hypochlorite of sodium (NaOCl), gibberellic acid (GA3), distilled water imbibition and stratification at 4 °C, in varying concentrations and exposure times (24 and 48 hours), using an experimental design completely random, with three repetitions of 50 seed each one. Statistical analysis...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Passiflora; Gulupa; Germination; Hydrogen peroxide; Gibberellic acid; Sodium hypochlorite.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452020000302003
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Análise de processos alternativos na preparação de esqueletos para uso didático - DOI: 10.4025/actascibiolsci.v30i4.5876 Biological Sciences
Silveira, Márcio José da; UEM; Teixeira, Gustavo Monteiro; UNESP/Botucatu; Oliveira, Edson Fontes de; UEM.
Laboratórios de Zoologia em Instituições de Pesquisa e Ensino têm demonstrado grande demanda por peças anatômicas para utilização em aulas práticas. No entanto, embora a bibliografia voltada para este tema não seja escassa, são raras as análises das relações custo/benefício de tais processos levando em consideração aspectos técnicos, como tempo de preparo, qualidade das peças e recursos materiais e humanos. Além de técnicas usualmente utilizadas, novos produtos de baixo custo, não-citados na literatura e encontrados facilmente no mercado, também foram testados. O principal objetivo deste trabalho foi a elaboração e organização de informações sobre técnicas alternativas de maceração de peças ósseas para coleções didáticas, realizando análises comparativas...
Palavras-chave: Maceração; Clarificação de esqueletos; Peróxido de hidrogênio; Suco de mamão maceration; Skeleton clarification; Hydrogen peroxide; Papaya juice.
Ano: 2008 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/5876
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Experimental study on the performance of microwave assisted Hydrogen peroxide (H2O2) pretreatment of rice straw CIGR Journal
Singh, Renu; Tiwari, Sapna; Srivastava, Monika; Shukla, Ashish.
Biofuels are essentials as they can provide impending substitute for fossil fuels.  Rice straw has gained much attention from researchers because of its usability as a potential feedstock for bioethanol production.  Pre-treatments are crucial for enzymatic hydrolysis of rice straw.  In this study, combination of microwave and hydrogen peroxide (H2O2) is employed for the delignification of rice straw.  The Response surface methodology is used to optimize the pretreatment conditions with respect to H2O2 concentration, microwave power and irradiation time.  Under optimum conditions, maximum reducing sugar obtained through microwave assisted H2O2 is 1,453.64 µg/mL.  The chemical and morphological analysis ascertained that the surface of the samples treated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biofuel; Bio-ethanol Biomass Enzymatic hydrolysis; Rice straw; Biofuel; Microwave; Hydrogen peroxide.
Ano: 2014 URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/2633
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Low concentrations of doxycycline attenuates FasL-induced apoptosis in HeLa cells Biol. Res.
Yoon,Jung Mi; Koppula,Sushruta; Huh,Se Jong; Hur,Sun Jin; Kim,Chan Gil.
BACKGROUND: Doxycycline (DC) has been shown to possess non-antibiotic properties including Fas/Fas Ligand (FasL)-mediated apoptosis against several tumor types in the concentration range of 10-40 &#956;g/mL. However, the effect of DC in apoptotic signaling at much low concentrations was not studied. METHODS: The present study investigated the attenuation effect of low dose of DC on FasL-induced apoptosis in HeLa cell by the methods of MTT assay, fluorescence microscopy, DNA fragmentation, flow cytometry analysis, and western blotting. RESULTS AND CONCLUSION: In the present findings we showed that low concentration of DC (<2.0 &#956;g/mL) exhibited protective effects against FasL-induced apoptosis in HeLa cells. FasL treatment to HeLa cells...
Tipo: Journal article Palavras-chave: Apoptosis; Caspase; Cisplatin; FasL; Hydrogen peroxide; Tetracycline.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100038
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Hydrogen Peroxide Induced Oxidative Damage on Mineral Density and Mechanical Properties of Bone BABT
Cicek,Ekrem; Cakmak,Enes.
ABSTRACT Hydrogen peroxide is a common reactive oxygen species involved in the catalytic mechanism though it is toxic to cells due to its oxidative nature. This work investigates the effects of hydrogen peroxide induced oxidative damage on bone mineral density and mechanical properties of bone which is primarily a composite material composed primarily of collagen fibers and biominerals. Sheep leg bones were exposed to hydrogen peroxide for a week. Bone mineral density was measured by using dual energy X-ray absorptiometry. Compressive modulus tests were applied to bone in order to determine mechanical properties. Our study shows that the hydrogen peroxide induced oxidative stress has negative effects on bone mineral density and stiffness. We observed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bone mineral density; Compressive modulus; Hydrogen peroxide; Reactive oxygen species.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100320
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Interference of a commercial catalase preparation in laccase and peroxidase activities BABT
Ballaminut,Nara; Yamanaka,Renata; Machado,Katia Maria Gomes.
The influence of commercial catalase preparations (fungal and bovine origin) on laccase and peroxidase activity assays was evaluated using enzymatic extracts obtained from several basidiomycetes grown under different culture conditions. No hydrogen peroxide was detected in the extracts. Inhibition of laccase activity by 40 to 80% was related to the catalase source. In addition, oxidation of the substrate (ABTS) by fungal catalase in the absence of the enzymatic extract from basidiomycetes was observed. The results demonstrated the need for the evaluation of interference of the commercial catalase preparation when its use was required in the reaction mixture.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Peroxidase; Fungi; Basidiomycetes; Hydrogen peroxide; Enzymatic inhibition.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500017
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Characterization of native and oxidized starches of two varieties of Peruvian carrot (Arracacia xanthorrhiza, B.) from two production areas of Paraná state, Brazil BABT
Matsuguma,Luciana Shizue; Lacerda,Luiz Gustavo; Schnitzler,Egon; Carvalho Filho,Marco Aurélio da Silva; Franco,Célia Maria Landi; Demiate,Ivo Mottin.
Two commercial varieties of Peruvian carrot ('Amarela de Carandaí' and 'Senador Amaral') were processed into flour, starch and bagasse and chemically evaluated. The starch was extracted, modified with H2O2 and characterized by the physicochemical methods. By using the methylene blue dyeing, the granules of the modified starches showed intense blue color. The carboxyl content, the reducing power and the amount of the water liberated from the pastes after the freeze-thawing were higher for the oxidized starches and their pastes were clearer than those of the native starches of the two varieties from the two production areas. The RVA viscoamylography showed that the modified starches had lower viscosities with differences between the varieties. In the thermal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Peruvian carrot varieties; Starch; Hydrogen peroxide; Technological properties.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300022
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The effect of phosphoethanolamine intake on mortality and macrophage activity in mice with solid ehrlich tumors BABT
Arruda,Maria Sueli Parreira de; Correa,Mara Adriana; Venturini,James; Félix,Maira Camila; Rosis,Ana Maria Bormio De; Galhiane,Mário Sérgio; Chierice,Gilberto Orivaldo; Costa,Maria Renata Sales Nogueira.
The aim of the present study was to examine the effect of a diet rich in synthetic PEtn on the metabolism macrophages of tumor-bearing mice. The results demonstrated that PEtn increased the animals' survival time. In addition, the treated animals released smaller amounts of hydrogen peroxide (H2O2) and nitric oxide (NO) than the non-treated animals, particularly after day 14. From the results it could be concluded that H2O2 and NO were important in the modulation of neoplastic growth, and pointed to a promising role of PEtn in the control of human neoplasms.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phosphoethanolamine; Ehrlich tumor; Nitric oxide; Hydrogen peroxide; Lipids.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600016
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Efeito do etanol e do peróxido de hidrogênio intragástrico sobre a mucosa gástrica de rã (Rana catesbeiana, SHAW) BABT
Estivallet,Hidê Virgínia; Rodrigues,Maria Inês; Marroni,Norma Possa.
This work investigated the frog gastric mucosa response to hydrogen peroxide and ethanol induced injury. Acid and mucus secretion were estimated "in vitro" in control animals with intragastric absolute ethanol (1ml/30min. or 2ml/60min.) and hydrogen peroxide. The gastric mucosa morphological conditions were assessed "in vivo", concerning lesion area, pH and mucus. Ethanol (1ml/30min.) was observed to cause hyperemia, cell damage, rupture, edema, erosions, necrosis in gastric mucosa and significant increase in acid secretion. Absolute ethanol (2ml/60min.) caused a decrease in acid secretion due to alcalinization and an increase of mucus and pH. Intragastric hydrogem peroxide provoked gastric unwrinkling and hyperemia, acid secretions were not increased,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gastric acid secretion; Mucus; Ethanol; Hydrogen peroxide; Frog.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000100007
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Effects of hydrogen peroxide mouthwash on preventing ventilator-associated pneumonia in patients admitted to the intensive care unit BJID
Nobahar,Monir; Razavi,Mohammad Reza; Malek,Farhad; Ghorbani,Raheb.
Abstract Aims The aim of the study was to determine the effect of hydrogen peroxide (HP) mouthwash on the incidence of ventilator associated pneumonia (VAP) in patients admitted to the intensive care unit (ICU). Methods This was a randomized clinical trial conducted on 68 patients. The intervention group used 3% HP as mouthwash and the control group used mouthwashes with 0.9% normal saline (NS) twice a day. Data were collected using a questionnaire and the Modified Clinical Pulmonary Infection Score (MCPIS). MCPIS includes five items, body temperature: white blood cell count, pulmonary secretions, the ratio of pressure of arterial oxygen (PaO2) to fraction of inspired oxygen (FiO2), and the chest X-ray. Each of these items scored 0–2. Scores ≥6 were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nosocomial infection; Ventilator associated pneumonia; Hydrogen peroxide; Intensive care unit.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000500444
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Age-related changes and effects of regular low-intensity exercise on gait, balance, and oxidative biomarkers in the spinal cord of Wistar rats BJMBR
Silveira,E.M.S.; Kroth,A.; Santos,M.C.Q.; Silva,T.C.B.; Silveira,D.; Riffel,A.P.K.; Scheid,T.; Trapp,M.; Partata,W.A..
The present study aimed to analyze age-related changes to motor coordination, balance, spinal cord oxidative biomarkers in 3-, 6-, 18-, 24-, and 30-month-old rats. The effects of low-intensity exercise on these parameters were also analyzed in 6-, 18-, and 24-month-old rats. Body weight, blood glucose, total cholesterol, and high-density lipoprotein (HDL) cholesterol were assessed for all rats. The soleus muscle weight/body weight ratio was used to estimate skeletal muscle mass loss. Body weight increased until 24 months; only 30-month-old rats exhibited decreased blood glucose and increased total cholesterol and HDL cholesterol. The soleus muscle weight/body weight ratio increased until 18 months, followed by a small decrease in old rats. Exercise did not...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Superoxide anion generation; Hydrogen peroxide; Total thiol content; Lipid hydroperoxide levels; Total antioxidant capacity; Superoxide dismutase.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000700608
Registros recuperados: 36
Primeira ... 12 ... Última
 

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