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In vitro shoot induction and multiplication from nodal segments of adult Ginkgo biloba plants Horticultura Brasileira
Mantovani,Nilton César; Grando,Magali F; Xavier,Aloisio; Otoni,Wagner C.
The in vitro performance of herbaceous and woody nodal segments from adult plants and the effect of hydrolyzed casein (HC 500 mg L-1), kinetin (KIN; 6-furfurylaminopurine 0.46 and 4.65 µM) and activated charcoal (AC 1.5 g L-1) were evaluated upon new shoots induction and development, and to establish a system of in vitro propagation from adult plants of Ginkgo biloba. Woody nodal segments did not produce axillary shoots and presented 100% of bacterial and fungal contamination in culture. However, nodal segments from herbaceous shoots were successfully disinfected and displayed high in vitro morphogenic capacity. The HC was essential for the axillary shoots induction and further multiplication, stimulating shoot formation in 85% of the cultured nodal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hydrolyzed casein; Activated charcoal; Kinetin; Multiple shoots; Herbaceous and woody branches.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362013000200003
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