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Paula,Márcia Beatriz Cardoso de; Yokosawa,Jonny; Coutinho,Márcio Danilo Botrel; Silva,Paulo Lourenço; Ferraz,Rogério Avelar; Oliveira,Thelma Fátima Mattos; Queiróz,Divina Aparecida Oliveira. |
In order to identify and characterize the agent of a suggestive clinical case of Gumboro disease (GD) that affected a 34-day-old broiler flock in Buriti Alegre (Goias State, Midwestern Brazil) in the year 2001, we carried out a combination of classic and modern virological methods. Histopathological analysis of the bursa revealed necrosis, presence of depleted follicles, some infiltration of heterophils, edema and formation of cystic cavities that are compatible with lesions observed in GD. Inoculation of embryonated eggs of specific pathogen-free (SPF) chickens with macerated bursa suspension resulted in embryo mortality and lesions which were also compatible with those caused by IBDV. A sample of bursa was submitted to a nested reverse... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chicken broiler flock; Gumboro disease; Infectious bursal disease virus; IBDV; RT-PCR; Molecular characterization. |
Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000300016 |
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Santos,Helton Fernandes dos; Lovato,Luciane Teresinha; Flôres,Maristela Lovato; Trevisol,Iara Maria; Mazzutti,Ketty Cristina; Pan,Kleitton Adolfo. |
No Brasil, a população de aves conhecida como galinhas de terreiro encontra-se fora do sistema de biosseguridade aplicada às criações comerciais. Para investigar a presença de anticorpos contra alguns vírus específicos nesta população, foram coletadas amostras de sangue de 867 aves não-vacinadas em 60 propriedades de 22 municípios do Estado do Rio Grande do Sul, Brasil. O soro foi testado para a presença de anticorpos contra o vírus da bronquite infecciosa das galinhas (IBV), reovírus aviário (ARV) e o vírus da doença infecciosa da bolsa (IBDV) pela técnica de soroneutralização. Anticorpos contra IBV foram detectados em 65% (564/867) das amostras, contra ARV em 21,6% (187/867) e contra IBDV em 80,2% (695/867) das aves. Todas as propriedades avaliadas... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: IBV; ARV; IBDV; Epidemiologia; Ocorrência; Galinhas de terreiro. |
Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000700020 |
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Michell,BC; Gomes,AD; Baião,NC; Resende,M; Lara,LJC; Martins,NRS. |
The interference of low or high maternal antibodies titers on the attenuated infectious bursal disease (IBD) virus (IBDV) vaccine infection and its effects on the performance of broilers vaccinated at the 18th day of incubation (in ovo), at one day of age (subcutaneously-SC), or at 15 days of age (drinking water-DW) were investigated. After a series of three live vaccinations, breeders were given or not an IBD oil emulsion vaccine (IBD-OEV) prior to sexual maturity. At day 18 of incubation (in ovo), a commercial vaccine containing HVT and an intermediate IBDV strain or the single HVT vaccine was given. An intermediate IBDV vaccine was given SC at one day of age, or at 15 days of age via DW. The progeny of unvaccinated breeders presented higher neutralizing... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Attenuated IBDV; Attenuated IBDV neutralization; Broiler chicken; Infectious bursal disease; Infectious bursal disease virus; IBDV; In-ovo vaccination; Maternal antibody; RT-PCR; Serum neutralization; Vaccination versus performance. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2009000100009 |
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Naseem,S; Rahman,SU; Shafee,M; Sheikh,AA; Khan,A. |
This study was designed to investigate the effects of a probiotic plus an immunomodulatory product on the growth performance, immune response and net economic returns of broiler chicks vaccinated against Infectious bursal diseases virus (IBDV). A flock of 350 day-old chicks were equally and randomly distributed in seven groups, each comprising 50 birds. A mixture of microorganisms (¹Probiotic) or Cyclophosphamide (immune suppressor) was offered through feed supplementation to different groups. The Probiotic, consisting of Lactobacillus species, Bifidobacterium, Streptococcus salivarius and Enterococcus faecium, in addition to Aspergillus oryza and Candida pintolopessii. Compared with control treatment group, the probiotic-supplemented chicks had better... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Probiotic; Cyclophosphamide; Mortality; Morbidity; IBDV. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2012000200004 |
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Agüero,J.A; Ceballo,Yanaysi; González,Yadmell; Martínez,Siomara; Enríquez,G.A. |
El virus de la enfermedad infecciosa de la bolsa (IBDV), un miembro de la familia Birnaviridae, es un virus ARN de doble cadena, capaz de causar considerables pérdidas económicas al inducir destrucción de la bolsa, inmunosupresión y alta mortalidad en pollos jóvenes. La cápsida del virus está formada por las proteínas estructurales VP2 y VP3. Se amplificó por PCR el gen vp3 (870pb), a partir del vector pcDNA3-Poly que contiene el gen que codifica para la poliproteína (pVP2-VP4-VP3) del virus. El producto de PCR se clonó en el plásmido pQE30 y se expresó en la cepa M15 de E. coli a altos niveles como una proteína de fusión con una cola de hexahistidina. Así se obtuvo una proteína antigénicamente activa, como se demostró por Western-Blot con un anticuerpo... |
Tipo: Journal article |
Palavras-chave: VP3; IBDV; Cápsida; Proteína recombinante; IMAC. |
Ano: 2007 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2007000100007 |
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