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Registros recuperados: 27
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Separation of the genera in the subtribe Cassiinae (Leguminosae: Caesalpinioidae) using molecular markers Acta Botanica
Acharya,Laxmikanta; Mukherjee,Arup Kumar; Panda,Pratap Chandra.
Random amplified polymorphic DNA (RAPD), Inter simple sequence repeat (ISSR) and Amplified fragment length polymorphism (AFLP) markers were used to verify the segregation of the genus Cassia L. senso lato into three distinct genera namely Chamaecrista Moench., Senna P. Mill. and Cassia L. sensostricto Eighteen representatives of the three taxa were characterized using the molecular markers. 25 RAPD, six ISSR primers and six AFLP primer combinations resulted in the amplification of 612, 115 and 622 bands (loci) respectively. Most of the loci are found to be polymorphic, showing high degrees of genetic diversity among the different taxa studied. The dendrogram constructed on the basis of the RAPD, ISSR and AFLP data using SHAN clustering, divided Cassia L....
Tipo: Info:eu-repo/semantics/article Palavras-chave: AFLP; Cassia; ISSR; Molecular phylogeny; RAPD.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062011000100026
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Genetic diversity of populations of the dioecious Myrsine coriacea (Primulaceae) in the Atlantic Forest Acta Botanica
Paschoa,Roberta Pena da; Christ,Jheniffer Abeldt; Valente,Cecília Silva; Ferreira,Marcia Flores da Silva; Miranda,Fábio Demolinari de; Garbin,Mário Luís; Carrijo,Tatiana Tavares.
ABSTRACT Although a species’ sexual system may influence the genetic diversity of its populations in their natural environment, there have been few such studies involving indigenous species of the Atlantic Forest. Here we study Myrsine coriacea, a dioecious tree widely used in reforestation programs despite a lack of information about its natural interpopulation genetic variation. To address this knowledge gap, intra- and interpopulation genetic diversity were measured for male and female individuals of ten natural populations using ISSR markers. Greater intrapopulation genetic diversity indicated interpopulation gene flow, regardless of isolation and distance between populations. Multivariate analyses detected significant differences in genetic diversity...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Capororoca; Conservation; ISSR; Myrsinaceae; Rapanea.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062018000300376
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Genetic evidence of multiple reproductive strategies in a microendemic and threatened cactus (Cactaceae: Discocactus Pfeiff) in Bahia, Brazil Acta Botanica
Jesus,Izabela Santos Dias de; Conceição,Leila Patricio; Schnadelbach,Alessandra Selbach; Assis,José Geraldo de Aquino; Carvalho,Maria Luiza Silveira de.
ABSTRACT Discocactus zehntneri subsp. petr-halfari, an endangered taxon, is represented by a single population in an anthropized area of Bahia, Brazil, where it is suffering due to extreme extractivism. Thus, information about this cactus, such as its reproductive patterns, is urgently needed to support conservation strategies. A population genetics approach was used to determine if this subspecies has a preferential pattern of reproduction. We sampled 18 individuals, both with and without connection to parental plants, from five clumps and assessed their diversity and genetic structure using five ISSR markers. The results revealed two clumps that are genetically supported by the presence of genetically equal individuals. The other three groups presented...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Discocactus; ISSR; Genetic variabilty; Microendemism; Reproductive strategies.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062019000300437
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Assessment of Genetic Fidelity of in vitro Raised Plants in Swertia chirayita through ISSR, RAPD analysis and Peroxidase Profiling during Organogenesis BABT
Sharma,Vikas; Belwal,Nidhi; Kamal,Barkha; Dobriyal,Anoop Kumar; Jadon,Vikash Singh.
ABSTRACT An efficient in vitro regeneration protocol for medicinally important herb Swertia chirayita was developed and the genetic fidelity was assessed using RAPD and ISSR markers. The best shoot regeneration was observed on MS basal supplemented with 1.0 mg/L Benzyl amino purine (BAP) in combination with Indole-3-acetic acid (IAA) (0.5 mg/L) that resulted in the increase by multiplication rate (7.65) with an average of 33.33 numbers of shoots and average shoot length of 2.70 cm. It was further enhanced by the addition of adenine sulfate (0.007%) that resulted in an average of 42 shoots per clum with 4.13 cm of average shoot length and the increase in multiplication fold to 9.75 that further resulted in the reduced use of other cytokinins and auxins. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Swertia chirayita; Micropropagation; Adenine sulfate; Ascorbate peroxidase; RAPD; ISSR.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100434
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Genetic Diversity of Some Capparis L. Species Growing in Syria BABT
Al- Safadi,Bassam; Faouri,Hussam; Elias,Rana.
This work investigated the genetic diversity and relationships among Capparis species growing in Syria using IRAP and ISSR techniques. Forty-seven samples of three Capparis species genotypes were collected from 21 different locations in Syria. The genotypes were morphologically identified based on the descriptions available in the literature. When IRAP technique was used, an average of 71.5% of the amplified fragments were polymorphic compared to 82.04% in ISSR. Morphological characterization along with the cluster and PCoA analyses of the data divided the studied genotypes into three groups. The groups included genotypes identified as Capparis spinosa L, C. sicula Duh., and C. aegyptia Lam. Based on the morphological description, molecular studies and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Capparis; Diversity; ISSR; IRAP; Syria.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600916
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Efficiency of RAPD and ISSR markers in assessing genetic variation in Arthrocnemum macrostachyum (Chenopodiaceae) BABT
Saleh,Basel.
The goal of this study was to investigate the molecular characterization of A. macrostachyum genotypes grown in the Western coast of Syria using RAPD and ISSR techniques. PCR amplifications with 20 RAPD primers gave an average of 9.25 selected markers/ primer, with a maximum of 17 (OPG05) and a minimum of four (OPA02). Percentage of polymorphic bands ranged from 40 to 100% according to RAPD primers tested. Among the 185 selected bands, 160 (84.96%) were polymorphic. The amplification with seven ISSR primers generated 88 bands, and 80 (90.91%) were polymorphic. (CA)8RG and (AG)8GTG ISSR primers tested in this study yielded highly informative patterns. RAPD and ISSR fragment sizes ranged from 0.2-3 kb. Based on this study, the use of RAPD and ISSR...
Tipo: Info:eu-repo/semantics/article Palavras-chave: A. macrostachyum; Genetic diversity; Genotype; ISSR; Polymorphism; RAPD.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500002
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Genetic diversity in natural populations of Hancornia speciosa Gomes: Implications for conservation of genetic resources Ciência e Agrotecnologia
Fajardo,Cristiane Gouvêa; Costa,Daniel Ferreira da; Chagas,Kyvia Pontes Teixeira das; Vieira,Fábio de Almeida.
ABSTRACT The continuing fragmentation of forests has been a threat to the maintenance of genetic resources. Genetic diversity is fundamental to the survival of species in natural environments in the long term, as well as being the basis for genetic improvement. The objective of this study was to evaluate the genetic diversity in natural populations of Hancornia speciosa and to contribute to the development of conservation strategies. We sampled 105 individuals of H. speciosa, distributed in seven populations. The ISSR (Inter-Simple Sequence Repeat) markers provided 70 loci, of which 81% were polymorphic. The mean genetic diversity of Nei (h) was 0.19, and the Shannon index (I) was 0.27. The h and I diversity indices ranged respectively from 0.16 to 0.24 in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apocynaceae; Genetic structure; Genetic bottleneck; ISSR; Mangabeira.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542018000600623
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Performance of the double-cross tomato hybrids from a partial diallel Ciência e Agrotecnologia
Matos,Rafael de; Resende,Juliano Tadeu Vilela de; Zeist,André Ricardo; Corte,Lígia Erpen-Dalla; Da-Silva,Paulo Roberto; Zeffa,Douglas Mariani.
ABSTRACT Knowledge of the combining ability of genotypes, genetic diversity, and heterosis play the paramount role in obtaining double-cross hybrids with high productivity. This study was aimed at evaluating these parameters in tomato genotypes using partial diallel crosses among the commercial single-cross hybrids, which verified the possibility of using double-cross hybrids for commercial purposes. Tomato genotypes included 15 double-cross hybrids, their genitors, and two commercial genotypes as checks. Of the fifteen double-cross hybrids of tomato, two crosses exhibited superiority, such as Aguamiel × Compack and Dominador × Compack, mainly for the total and commercial fruit yields. The phenotypic expression of the trait is controlled by the genes with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Solanum lycopersicum; ISSR; Molecular markers; Plant breeding; Heterosis..
Ano: 2021 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542021000100205
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BSA and molecular markers screening for salt stress tolerant mutant of Petunia obtained in in vitro culture Ciência Rural
Krupa-Małkiewicz,Marcelina; Bienias,Anna.
ABSTRACT: In this study, we performed BSA to identify genetic markers linked to salt tolerance. We tested the genetic diversity among four bulked DNA samples of EMS induced mutant clones and one bulked DNA sample of non-mutated clone of Petunia for salt tolerance in in vitro callus cultures using RAPD and ISSR markers. Out of the 36 RAPD and 16 ISSR primers identified, 25 and 13 were effectively used to amplify genomic DNA of all the five bulked samples, respectively. In total, 114 RAPD amplifications products were obtained, of which 28% were polymorphic and 2% were genotype-specific bands. Out of the 64 ISSR amplification products obtained, 51% were polymorphic and 1% was genotype-specific bands. Results of this study indicated the existence of two...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bulk segregant analysis; Chemical mutagenesis; ISSR; RAPD; Petunia × atkinsiana D. Don; Salt stress; Tissue culture..
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001200400
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Construction of a molecular identification profile of new varieties of Nierembergia linariaefolia by anchored microsatellites Electron. J. Biotechnol.
Pérez de la Torre,Mariana C.; Escandón,Alejandro S..
The objective of the present work was to establish the molecular identification profile for six new varieties of Nierembergia linariaefolia to incorporate the fingerprint, as complementary information to the standard registration data. Total DNA was extracted from young leaves following the protocol of the cetyltrimethylammonium bromide. Anchored microsatellites were used as molecular markers. The amplification reactions were carried out with seven primers. A total of 251 loci were detected, 98% of them were polymorphic. The average of polymorphic loci was 35 loci per primer and, 41 loci per genotype. Six out of the seven primers used discriminated all the individuals involved in the present study; consequently, it was possible to generate the molecular...
Tipo: Journal article Palavras-chave: ISSR; Ornamentals; Varietal identification.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300014
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Greenhouse evaluation on the performance of heat tolerant transgenic broccoli and genetic diversity analysis using inter simple sequence repeat (ISSR) markers Electron. J. Biotechnol.
Ravanfar,Seyed Ali; Aziz,Maheran Abdul; Shabanimofrad,Mahmoodreza; Samarfard,Samira.
Background: Broccoli, Brassica oleracea subsp. italica is one of the many valuable Brassica species which is still less cultured under in vitro condition. Heat tolerant transgenic and non-transgenic broccoli cv. Green Marvel plantlets with well-developed root system obtained through in vitro culture were transferred into disposable plastic pots containing sterilized potting mixture consisting of (peatgroTM) + coconut dust (2:1) and maintained in a growth chamber. Results: After one month, the hardened plantlets were transferred and maintained in a transgenic greenhouse. After four months of acclimatization in the transgenic greenhouse, the efficacy of HSP101 gene in increasing the heat tolerance of the transgenic broccoli was evaluated. Results showed that...
Tipo: Journal article Palavras-chave: AtHSP101 gene; Brassica oleracea; ISSR; Transgenic broccoli.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000500004
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Inter simple sequence repeats separate efficiently hemp from marijuana (Cannabis sativa L.) Electron. J. Biotechnol.
Hakki,Erdogan E; Kayis,Seyit A; Pinarkara,Emine; Sag,Ayla.
Cannabis sativa L. is a multiple-use plant that provides raw material for the production of seed oil, natural fiber for textiles, automotive and pulp industries. It has also been used in insulating boards, ropes, varnishes, animal feed, and as medicinal agents. Cannabis has potential to be used for phytoremediation: however, its cultivation is strictly controlled due to its psychoactive nature and usage in producing drugs such as marijuana, and hashish. In this study, psychoactive type Cannabis samples, which were seized from 23 different locations of Turkey, and nine hemp type Cannabis accessions, as well as an unknown accession were used. Our interest was to identify the genetic relatedness of the seized samples and to separate drug and hemp type plants....
Tipo: Journal article Palavras-chave: Cluster analysis; Hemp; ISSR; Marijuana; Principle coordinate analysis.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000400010
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High genetic diversity and small genetic variation among populations of Magnolia wufengensis (Magnoliaceae), revealed by ISSR and SRAP marker Electron. J. Biotechnol.
Chen,Liyuan; Chen,Faju; He,Suichao; Ma,Luyi.
Background Genetic diversity and genetic variation of 10 populations and subpopulations of Magnolia wufengensis, a new and endangered endemic species, were examined by inter simple sequence repeat (ISSR) and sequence-related amplified polymorphism (SRAP) molecular markers. Compared with other endangered endemic Magnolia taxa, M. wufengensis holds a relatively high level of genetic variation. Result Total genetic diversity was found to be 87.7% for ISSR and 88.0% for SRAP markers. For polymorphic loci (P), the effective mean number of alleles (Ae) was 1.414 for ISSR markers and 1.458 for SRAP markers, while the mean expected heterozygosity (H) was 0.256 using ISSR and 0.291 for SRAP markers. Within-population variation was estimated for P as 74.9% using...
Tipo: Journal article Palavras-chave: Genetic structure; ISSR; Population genetic diversity; SRAP.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000600003
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Genetic diversity and structure of Atta robusta (Hymenoptera, Formicidae, Attini), an endangered species endemic to the restinga ecoregion Genet. Mol. Biol.
Reis,Evelyze Pinheiro dos; Salomão,Tânia Maria Fernandes; Campos,Lucio Antonio de Oliveira; Tavares,Mara Garcia.
The genetic diversity and structure of the ant Atta robusta were assessed by ISSR (inter-simple sequence repeats) in 72 colonies collected from 10 localities in the Brazilian states of Espírito Santo (48 colonies) and Rio de Janeiro (24 colonies). The ISSR pattern included 67 bands, 51 of them (76.1%) polymorphic. Analysis of molecular variance (AMOVA) revealed a high level (57.4%) of inter-population variation, which suggested a high degree of genetic structure that was confirmed by UPGMA (unweighted pair-group method using an arithmetic average) cluster analysis. The significant correlation between genetic and geographic distances (r = 0.64, p < 0.05) indicated isolation that reflected the distance between locations. Overall, the populations were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endemism; Endangered; ISSR; Population genetics; Restinga.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400015
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Genetic variation in cultivated Rheum tanguticum populations Genet. Mol. Biol.
Hu,Yanping; Xie,Xiaolong; Wang,Li; Zhang,Huaigang; Yang,Jian; Li,Yi.
To examine whether cultivation reduced genetic variation in the important Chinese medicinal plant Rheum tanguticum, the levels and distribution of genetic variation were investigated using ISSR markers. Fifty-eight R. tanguticum individuals from five cultivated populations were studied. Thirteen primers were used and a total of 320 DNA bands were scored. High levels of genetic diversity were detected in cultivated R. tanguticum (PPB = 82.19, H = 0.2498, H B = 0.3231, I = 0.3812) and could be explained by the outcrossing system, as well as long-lived and human-mediated seed exchanges. Analysis of molecular variance (AMOVA) showed that more genetic variation was found within populations (76.1%) than among them (23.9%). This was supported by the coefficient...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cultivated populations; Genetic variation; ISSR; Polygonaceae; Rheum tanguticum Maxim. ex Balf.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400010
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Genetic relationship in Coffea species and parentage determination of interspecific hybrids using ISSR (Inter- Simple Sequence Repeat) markers Genet. Mol. Biol.
Ruas,Paulo M.; Ruas,Claudete F.; Rampim,Leandro; Carvalho,Valdemar P.; Ruas,Eduardo A.; Sera,Tumoru.
Inter-simple sequence repeat (ISSR) markers were used to evaluate genetic divergence among eight Coffea species and to identify the parentage of six interspecific hybrids. A total of 14 primers which contained different simple sequence repeats (SSR) were used as single primers or combined in pairs and tested for PCR amplifications. Two hundred and thirty highly reproducible fragments were amplified, which were then used to estimate the genetic similarity and to cluster the Coffea species and hybrids. High levels of interspecific genetic variation were revealed. The dinucleotide motif (GA)9T combined with other di- tri- and tetra-nucleotides produced a greater number of DNA fragments, mostly polymorphics, suggesting a high frequency of the poly GA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coffea; Coffee breeding; DNA markers; ISSR.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300017
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Determination of genetic variation in Rhodiola crenulata from the Hengduan Mountains Region, China using inter-simple sequence repeats Genet. Mol. Biol.
Lei,Yidong; Gao,Hong; Tsering,Tashi; Shi,Suhua; Zhong,Yang.
The plant Rhodiola crenulata is a perennial herbaceous species distributed in the plateau region of southwestern China, especially the Hengduan Mountains region. It has been one of the most important traditional herbal remedies in Tibet for more than one thousand years, but the accelerated and uncontrolled collection of this plant since the 1980s has lead to deforestation. We used inter-simple sequence repeats (ISSR) to assess levels of genetic variation in R. crenulata from nine diverse natural populations in eastern Tibet and northern Yunnan, the first time such a study has been carried out. The 12 primers we used were able to detect 184 polymorphic loc. Analysis of molecular variance (AMOVA) indicated that species level genetic diversity was relatively...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic diversity; Hengduan Mountains region; ISSR; Rhodiola crenulata.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200023
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Clonal diversity and conservation genetics of the medicinal plant Carapichea ipecacuanha (Rubiaceae) Genet. Mol. Biol.
Oliveira,Luiz Orlando de; Venturini,Bruna Afonso; Rossi,Ana Aparecida Bandini; Hastenreiter,Saulo Santos.
The roots of the understorey shrub Carapichea ipecacuanha (ipecac) have medicinal properties, and the uprooting of wild plants has supplied most of the world demand for this species. Although under severe population decline, C. ipecacuanha lacks legal protection. In the wild, the aerial stems of ipecac clump together to form clusters with well-defined borders. Cluster size may range from several to hundreds of aerial stems. To investigate the extent of clonality among aerial stems in ipecac clusters, we sampled 50 wild clusters (a total of 291 aerial stems) and screened them with 89 inter-simple sequence repeat (ISSR) markers. The 291 aerial stems were grouped into 42 putative clones. The clonal groups generally consisted of aerial stems from the same...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Clonal propagation; Genetic diversity; ISSR; Poaia; Psychotria ipecacuanha.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100016
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Development of sequence characterized amplified region markers from intersimple sequence repeat fingerprints for the molecular detection of toxic phytoplankton Alexandrium catenella (Dinophyceae) and pseudo-Nitzschia pseudodelicatissima (Bacillariophyceae) from French coastal waters ArchiMer
Bornet, Benjamin; Antoine, Elisabeth; Francoise, Sylvaine; Marcaillou-le Baut, Claire.
Harmful algal blooms are a serious threat to shellfish farming and human health all over the world. The monitoring of harmful algae in coastal waters originally involved morphological identification through microscopic examinations, which was often difficult unless performed by specialists and even then often did not permit identification of toxic species. More recently, specific molecular markers have been used to identify specific phytoplankton species or strains. Here we report on the use of the intersimple sequence repeat (ISSR) technique to develop specific sequence characterized amplified region markers (SCAR) and to identify with these tools two toxic species in French coastal waters, the diatom Pseudo-nitzschia pseudodelicatissima (Hasle) Hasle and...
Tipo: Text Palavras-chave: SCAR; Pseudo nitzschia; Monitoring; Molecular identification; ISSR; Alexandrium.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-780.pdf
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ISSR as new markers for genetic characterization and evaluation of relationships among phytoplankton ArchiMer
Bornet, Benjamin; Antoine, Elisabeth; Bardouil, Michele; Marcaillou-le Baut, Claire.
In order to increase the molecular tools and markers needed for the identification of phytoplankton species, the inter simple sequence repeat ( ISSR) fingerprinting was adapted to micro-algae and its use in genetic analysis was demonstrated. Twelve strains, 6 Alexandrium, 4 Pseudo-nitzschia, 1 Skeletonema and 1 Tetraselmis were analysed for the first time with ISSR amplifications. The patterns were highly polymorphic and very reproducible. The 6 primers gave 223 polymorphic markers that clearly and easily distinguished all 12 strains ( mainly toxic ones) and gave 187 polymorphic markers among the Alexandrium and the Pseudo-nitzschia species. ISSR amplifications also indicated a large occurrence of simple sequence repeat ( SSR) in phytoplankton genomes,...
Tipo: Text Palavras-chave: Tetraselmis; Skeletonema; Pseudo nitzschia; ISSR; Genetic characterization and diversity; Fingerprints; Alexandrium.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-516.pdf
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