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| PAIVA,DANIELA IVANA; CASCALES,JIMENA; ROSETTI,MARÍA EVA NATALIA; SCHERER,RAFAEL ALEJANDRO; GAUCHAT,MARÍA ELENA; GOTTLIEB,ALEXANDRA MARINA. |
| Abstract We report the first comprehensive multilocus molecular characterization of cultivated Ilex paraguariensis plants belonging to a breeding program. Using nuclear and homologous chloroplastidic molecular markers, we have genotyped 158 plants from four plantation sites. Analyses of the nuclear data (187 variable dominant loci) allowed detecting high diversity (0.569), the occurrence of four distinct genetic clusters, and a low but significant differentiation among sites. Additionally, 20 chloroplastidic alleles were identified applying five microsatellite polymorphic markers, and a high chloroplastidic diversity was recognized (0.505); two haplogroups were distinguished amongst the 63 haplotypes detected. Our results from both nuclear and plastidic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chloroplastidic microsatellites; Genetic diversity; Genotyping; ISSR markers. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000100717 |
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| Nascimento,Marcília A.; Batalha-Filho,Henrique; Waldschmidt,Ana M.; Tavares,Mara G.; Campos,Lucio A.O.; Salomão,Tânia M.F.. |
| For a study of diversity and genetic structuring in Melipona quadrifasciata, 61 colonies were collected in eight locations in the state of Minas Gerais, Brazil. By means of PCR analysis, 119 ISSR bands were obtained, 80 (68%) being polymorphic. He and H B were 0.20 and 0.16, respectively. Two large groups were obtained by the UPGMA method, one formed by individuals from Januária, Urucuia, Rio Vermelho and Caeté and the other by individuals from São João Del Rei, Barbacena, Ressaquinha and Cristiano Otoni. The Φst and θB values were 0.65 and 0.58, respectively, thereby indicating high population structuring. UPGMA grouping did not reveal genetic structuring of M. quadrifasciata in function of the tergite stripe pattern. The significant correlation between... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hymenoptera; Melipona; Genetic differentiation; ISSR markers; Population structure. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200031 |
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| Palai,SK; Rout,Gyana Ranjan. |
| Chrysanthemum is the important cut flower after rose among the ornamental plants traded in the global flower market. It is propagated vegetatively and also has a strong sporophytic self-incompatibility system as shown by all members of Asteraceae family. Morphologically, the petal numbers and flower colours present maximum variation when compared to existing varieties. Twenty Inter Simple Sequence Repeat primers were used to detect the new variety of Chrysanthemum developed through spontaneous sporting. The results indicate that the rate of polymorphism showed significant differences as compared to other existing varieties. The average number of amplification products per primer was eight. The size of ISSR amplified fragments varied from 0.25 - 2.4 Kbp.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chrysanthemum; Sporting; Variety identification; ISSR markers. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362011000400029 |
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| Hawerroth,Maraisa Crestani; Bordallo,Patricia do Nascimento; Oliveira,Luis Cláudio Pessoa; Vale,Egnesio Holanda; Vidal Neto,Francisco das Chagas; Melo,Dheyne Silva. |
| Abstract: The objective of this work was to evaluate the use of RAPD and ISSR molecular markers to determine the genetic variability among cashew (Anacardium spp.) genotypes, and to indicate possible promising crosses based on cashew genetic variability and phenotypic performance. Ten hybrids - derived from the crosses CCP 76 x BGC 589, CCP 76 x BRS 226, CCP 76 x HAC 276-1, CCP 76 x Embrapa 51, CCP 76 x BRS 253, CCP 76 x HAC222-4, and BRS226 x Embrapa 51 - and their parents were assessed at the molecular level. The hybrids were evaluated for nut yield, mean nut weight, bored nuts, and powdery mildew on nuts (scale 0-4). The RAPD and ISSR markers were efficient in the determinaton of the genetic variability among cashew genotypes, allowing of the grouping... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anacardium; Cashew breeding program; ISSR markers; Nut health; RAPD markers. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2019000102802 |
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| Jimenez,Luis Huala; Ranz,Ramón Rebolledo; López,Rubén Carrillo; Elgueta,Mario; Honorato,Marco Paredes. |
| Abstract Calvertius tuberosus (Curculionidae) lives exclusively on Araucaria araucana trees (commonly known as pehuen) in southern Chile. In this study, morphometric and molecular genetic analyses of Andean and coastal populations of C. tuberosus were performed to evaluate evolutionary divergence associated with the discontinuity of the Araucaria forest between the coastal and Andean regions. Specimens of C. tuberosus were collected in Nahuelbuta National Park, Villa Las Araucarias, and Malalcahuello National Reserve and were classified and stored at the Animal Biotechnology Researching Laboratory (LINBA), University of La Frontera, Temuco, Chile. Thirteen morphometric parameters and the expression patterns of ISSR (inter-simple sequence repeat) markers... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Araucaria forests; ISSR markers; Morphometry. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262018000200119 |
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