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The relationship between stem vessel parameters and the development of strata in the early stages of secondary forest succession in Amazonia Acta Amazonica
TSUCHIYA,Akio; HIRABUKI,Yoshihiko; NISHIZAWA,Toshie; LISBOA,Pedro Braga; SILVA,Carlos Rosário da.
In order to compare the development of strata in the early stages of secondary forest succession with vessel parameters of the tree species, a forest inventory was carried out in 4-year (Q1: 48 m2), 11-year (Q2: 400 m2) and 20-year (Q3: 400 m2) forests and vessel parameters were investigated from stem cross sections of 18 species obtained in Q2. Thirty three species (21 families), 77 species (35 families), 39 species (20 families) were found in Ql, Q2, Q3, respectively. The percentage of dead individuals, dead stems and the percentage of individuals with multiple stems increased with time after clear cutting. Also, the total D2H of Q3 was 26.1 times that of Q1, and the development of strata started in Q2 and Q3. The image analysis of vessel size, area and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amazonia; Secondary forest; Strata; Image analysis; Vessels.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672002000200241
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Recent approaches for bell pepper seed vigor testing Agronomy
Dias, Marcos Altomani Neves; Mondo, Vitor Henrique Vaz; Cicero, Silvio Moure.
Seed vigor is a major attribute in the determination of whether a seed lot can be commercialized. Different physiological tests are used to assess seed vigor and are generally based on different concepts, which could lead to variable interpretations, particularly when comparing lots presenting different vigor levels. This study was conducted to compare the different approaches used to evaluate bell pepper seed vigor and verify the applicability of the Seed Vigor Imaging System (SVIS) for this purpose. The experiment was performed using four seed lots of bell pepper hybrids in two periods, and the evaluations involved germination, seedling emergence, seedling emergence speed index, saturated salt accelerated aging and image analysis. The use of seedling...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Seed analysis; SVIS; Image analysis; Seed quality.
Ano: 2014 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/18348
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Automated image analysis of seedlings for vigor evaluation of common bean seeds Agronomy
Gomes Junior, Francisco Guilhien; Universidade de São Paulo; Chamma, Helena Maria Carmignani Pescarin; Universidade de São Paulo; Cicero, Silvio Moure; Universidade de São Paulo.
Quickly and precise evaluations of the vigor of seeds are highly desirable for the success of quality control programs applied to the seed industry. The objective of this experiment was to determine the number of days necessary to calculate the vigor index of common bean seeds using an automated system of seedling images (SVIS®), and compare its results with the traditional tests of seed vigor. Samples of five seed lots of the IAC Carioca cultivar were submitted to germination, accelerated aging, seedling emergence in sand and electrical conductivity tests. The parameters measured by the Seed Vigor Imaging System (SVIS®) were mean seedling length, growth (ratio of the actual seedling growth to the maximum possible growth), uniformity of growth and the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phaseolus vulgaris L.; Image analysis; Quality control.
Ano: 2014 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/21957
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Gamma-ray computed tomography to evaluate changes in the structure of a clayey soil due to agricultural traffic - doi: 10.4025/actasciagron.v33i3.4727 Agronomy
Pires, Luiz Fernando; Universidade Estadual de Ponta Grossa.
A first generation gamma-ray computed tomography (CT) equipped with a 137Cs source was used to investigate changes in soil structure due to agricultural machinery traffic. Core samples with volume of 55 cm3 were collected at the surface (0-10 cm) of a Brazilian clayey soil. Significant alterations in soil structure occurred after two passages of tractor wheels, with important decreases in soil porosity. CT data allowed a continuous analysis of soil bulk density and also soil porosity distribution for 1.25 and 1.20 mm layers, which cannot be provided by traditional methods commonly used in soil physics.
Palavras-chave: 5.01.01.02-1; 3.09.01.00-6 gamma-ray attenuation; Soil bulk density; Soil porosity; Image analysis; Soil physics Física dos Solos gamma-ray attenuation; Soil bulk density; Soil porosity; Image analysis; Soil physics.
Ano: 2011 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/4727
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Heartwood and sapwood in eucalyptus trees: non-conventional approach to wood quality Anais da ABC (AABC)
CHERELLI,SABRINA G.; SARTORI,MARIA MÁRCIA P.; PRÓSPERO,ANDRÉ G.; BALLARIN,ADRIANO W..
ABSTRACT This study evaluated the quality of heartwood and sapwood from mature trees of three species of Eucalyptus, by means of the qualification of their proportion, determination of basic and apparent density using non-destructive attenuation of gamma radiation technique and calculation of the density uniformity index. Six trees of each species (Eucalyptus grandis - 18 years old, Eucalyptus tereticornis - 35 years old and Corymbia citriodora - 28 years old) were used in the experimental program. The heartwood and sapwood were delimited by macroscopic analysis and the calculation of areas and percentage of heartwood and sapwood were performed using digital image. The uniformity index was calculated following methodology which numerically quantifies the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gamma radiation; Image analysis; Eucalyptus; Density uniformity index.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000100425
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Knockdown of apoptosis-inducing factor disrupts function of respiratory complex I Biocell
Varecha,Miroslav; Páclová,Daniela; Procházková,Jirina; Matula,Pavel; Cmarko,Dusan; Kozubek,Michal.
Recent findings suggest that apoptotic protein apoptosis-inducing factor (AIF) may also play an important non-apoptotic function inside mitochondria. AIF was proposed to be an important component of respiratory chain complex I that is the major producer of superoxide radical. The possible role of AIF is still controversial. Superoxide production could be used as a valuable measure of complex I function, because the majority of superoxide is produced there. Therefore, we employed superoxide-specific mitochondrial fluorescence dye for detection of superoxide production. We studied an impact of AIF knockdown on function of mitochondrial complex I by analyzing superoxide production in selected cell lines. Our results show that tumoral telomerase-positive (TP)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Superoxide; Fluorescence microscopy; Image analysis; Mitochondria; Telomerase.
Ano: 2012 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000300004
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Relationship between DAPI-fluorescence fading and nuclear DNA content: An alternative method to DNA quantification? Biol. Res.
GALLARDO-ESCÁRATE,CRISTIAN; ÁLVAREZ-BORREGO,JOSUÉ; VON BRAND,ELISABETH; DUPRÉ,ENRIQUE; DEL RÍO-PORTILLA,MIGUEL ÁNGEL.
In observations by confocal or conventional fluorescence microscopy, important factors should be considered in order to obtain accurate images. One of them, such as the fluorescence bleaching from highest intensity to lowest signal of fluorescence is a common problem with several DNA fluorochromes and especially for DAPI stain. The fluorescence of DAPI fades rapidly when it is exposed to UV light, under optimal conditions of observation. Although the fading process can be retarded using a mounting medium with antifading reagents, the photochemical process underlying the fluorescence decay has not yet been fully explained. In addition, no relationship between fluorescence fading and nuclear DNA content has been tested. In order to test this relationship, we...
Tipo: Journal article Palavras-chave: Nuclear DNA content; Fluorescence fading; Image analysis; DAPI.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000100004
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Quantification of Japanese Quail Eggshell Colour by Image Analysis Biol. Res.
SEZER,METIN; TEKELIOGLU,OGUZ.
The Japanese quail lays eggs with colourful and patterned shells which make the eggshell colour difficult to classify. In this study, the method of measuring colour of patchy eggs using image analyses and its power to discriminate among individual variation were established. Estimated repeatability for egg colour and proportion of patterned areas was high (>0.58), suggesting intermedíate or high heritability of eggshell colour characteristics. Three components have been identified as significant in discriminant function analysis. These three components explained 91.4% of the total variance in egg colour characteristics. In cluster analysis, 78.3% of the eggs that were collected from 15 females were correctly classified. This study indicates that...
Tipo: Journal article Palavras-chave: Eggshell colour; Image analysis; Individual variation; Repeatability.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100010
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X-ray Microtomography to Quantify Morphological Sandstones Properties BABT
Pires,Luiz Fernando; Melo,Mário Sérgio de; Borges,Jaqueline Aparecida Ribaski; Heck,Richard John; Facin,Paulo Cesar.
Abstract The sandstones of the Furnas Formation in the State of Paraná, south of Brazil, are located in regions with great importance due to the natural heritage found in the rocky forms and associated flora and fauna. This paper presents a detailed analysis of the internal structure of diverse sandstones of the Furnas Formation. X-ray microtomography allowed 3D characterization of the number, pore size and shape distributions of the sandstones. Images with voxel size of 603 µm3 were utilized to evaluate the sandstone’s macroporosity. The main results obtained indicate that higher macroporosities were observed in coarser and some types of silicified sandstones. In general, fine sandstones were characterized by the largest number of pores. As a general...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Image analysis; Porous system; Sandstone structure; 3D analysis; Micromorphology.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100605
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Analysis of trophic responses in lesioned brain: focus on basic fibroblast growth factor mechanisms BJMBR
Chadi,G.; Fuxe,K..
The actions of fibroblast growth factors (FGFs), particularly the basic form (bFGF), have been described in a large number of cells and include mitogenicity, angiogenicity and wound repair. The present review discusses the presence of the bFGF protein and messenger RNA as well as the presence of the FGF receptor messenger RNA in the rodent brain by means of semiquantitative radioactive in situ hybridization in combination with immunohistochemistry. Chemical and mechanical injuries to the brain trigger a reduction in neurotransmitter synthesis and neuronal death which are accompanied by astroglial reaction. The altered synthesis of bFGF following brain lesions or stimulation was analyzed. Lesions of the central nervous system trigger bFGF gene expression by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Radioautography; In situ hybridization; Basic fibroblast growth factor; FGF receptor; Astrocyte; Image analysis; Central nervous system; Brain lesion.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000200007
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Applications of image analysis in the characterization of Streptomyces olindensis in submerged culture BJM
Pamboukian,Celso R. Denser; Guimarães,Luciana M.; Facciotti,Maria Cândida R..
The morphology of Streptomyces olindensis (producer of retamycin, an antitumor antibiotic) grown in submerged culture was assessed by image analysis. The morphology was differentiated into four classes: pellets, clumps (or entangled filaments), branched and unbranched free filaments. Four morphological parameters were initially considered (area, convex area, perimeter, and convex perimeter) but only two parameters (perimeter and convex perimeter) were chosen to automatically classify the cells into the four morphological classes, using histogram analysis. Each morphological class was evaluated during growth carried out in liquid media in fermenter or shaker. It was found that pellets and clumps dominated in early growth stages in fermenter (due to the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptomyces olindensis; Image analysis; Morphology.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100003
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Implementation and Evaluation of an Image Analysis System for Determining Viability of Pollen Grains in Temperate Rice Chilean J. Agric. Res.
Ortega B,Rodrigo; Aresti E,Macarena; Pereira R,Iris.
Geographical and climatic factors limit the production of temperate rice (Oryza sativa L.) in Chile. Large thermal fluctuations of air and water temperatures affect the viability of pollen, causing high levels of spikelet sterility (”blanking”), resulting in reduced yields per hectare. Counting viable pollen grains, at the time of anthesis, may allow determining, ahead, whether there will be yield losses by spikelet sterility; besides, knowing the potential pollen viability serves as a powerful tool for material selection in breeding programs, and for determining which management factors may influence ”blanking”. If performed manually, counting total and viable pollen grains can be extremely tedious, producing highly variable results. The present work had...
Tipo: Journal article Palavras-chave: Rice crop; Pollen counting; Image analysis; Anthesis; Spikelet sterility.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000100002
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Software Image J to study soil pore distribution Ciência e Agrotecnologia
Passoni,Sabrina; Borges,Fernando da Silva; Pires,Luiz Fernando; Saab,Sérgio da Costa; Cooper,Miguel.
In the soil science, a direct method that allows the study of soil pore distribution is the bi-dimensional (2D) digital image analysis. Such technique provides quantitative results of soil pore shape, number and size. The use of specific softwares for the treatment and processing of images allows a fast and efficient method to quantify the soil porous system. However, due to the high cost of commercial softwares, public ones can be an interesting alternative for soil structure analysis. The objective of this work was to evaluate the quality of data provided by the Image J software (public domain) used to characterize the voids of two soils, characterized as Geric Ferralsol and Rhodic Ferralsol, from the southeast region of Brazil. The pore distribution...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Soil structure; Image analysis; Thin section; Soil porous system; Soil pore size distribution; Ferralsols.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542014000200003
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Parameters based on X-ray images to assess the physical and physiological quality of Leucaena leucocephala seeds Ciência e Agrotecnologia
Medeiros,André Dantas de; Araújo,Joyce de Oliveira; León,Manuel Jesús Zavala; Silva,Laércio Junio da; Dias,Denise Cunha Fernandes dos Santos.
ABSTRACT Non-destructive and high performance analyses are highly desirable and important for assessing the quality of forest seeds. The aim of this study was to relate parameters obtained from semi-automated analysis of radiographs of Leucaena leucocephala seeds to their physiological potential by means of multivariate analysis. To do so, seeds from five lots collected from parent trees from the region of Viçosa, MG, Brazil, were used. The study was carried out through analysis of radiographic images of seeds, from which the percentage of damaged seeds (predation and fungi), and measurements of area, perimeter, circularity, relative density, and integrated density of the seeds were obtained. After the X-ray test, the seeds were tested for germination in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Forest species; Image analysis; Multivariate analysis; Relative density; Seed radiography.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542018000600643
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Evaluación de severidad de antracnosis en frutos de mango usando proyecciones geométricas obtenidas de imágenes digitales. Colegio de Postgraduados
Soto Rojas, Lauro.
Se desarrolló una metodología para estimar el porcentaje de severidad de antracnosis (Colletotrichum gloeosporioides) en frutos de mango (Manguifera indica L.). Se emplearon imágenes digitales para hacer una proyección geométrica, la cual es la representación extendida de la superficie del fruto. Se construyó un goniómetro para girar un fruto sobre su eje longitudinal y tomar una foto cada 90 grados hasta adquirir cuatro imágenes por fruto. Cada imagen fue segmentada manualmente para separar el área enferma de la superficie sana del fruto. Después se desarrolló un módulo en el programa estadístico R para procesar las imágenes digitales y hacer la proyección geométrica. Esta técnica puede emplearse en otros frutos, con forma predominantemente convexa, para...
Palavras-chave: Colletotrichum gloeosporioides; Manguifera indica; Enfermedades postcosecha; Estimación de severidad; Análisis de imágenes; Proyección geométrica; Post-harvest diseases; Assessment of severity; Image analysis; Geometric projection; Maestría; Fitopatología.
Ano: 2010 URL: http://hdl.handle.net/10521/296
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Propiedades hídricas de mezclas de sustratos con diferentes proporciones y tamaños de particula Colegio de Postgraduados
Hernández Escobar, Jorge Luis..
Se evaluaron las propiedades hídricas (retención de agua y capacidad de aire) de mezclas de sustratos (fibra de coco: FC, piedra pómez: PP y tezontle: T) con micromorfología de partículas y demanda hídrica de plantas de lechuga Var. Summertime. Los factores de estudio fueron: tamaño de partícula (1-2 mm y 2-3.36 mm) y proporción de mezcla (0:100, 25:75, 50:50, 75:25 y 100:0, FC:T y FC:PP, respectivamente). Se determinaron las curvas de retención de agua mediante el uso de un equipo de succión con embudos de placa filtrante. Se realizaron láminas delgadas de las mezclas de sustratos para evaluar la porosidad y la distribución de partículas. Las mezclas con mejor retención de agua y capacidad de aireación se probaron en plantas de lechuga bajo...
Tipo: Tesis Palavras-chave: Fibra de coco; Tezontle; Piedra pómez; Micromorfología; Análisis de imágenes; Retención de agua; Maestría; Edafología; Coconut fiber; Pumice; Micromorphology; Image analysis; Water holding..
Ano: 2009 URL: http://hdl.handle.net/10521/1500
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Microcartografía y patrones espectrales de la actividad biológica en secciones delgadas. Colegio de Postgraduados
Suástegui Méndez, Enrique.
Los estudios sobre la cuantificación de hongos se realizan de manera alterada, por lo que poco se conoce sobre la distribución de las esporas e hifas en el suelo y sus relaciones con rasgos edáficos de manera precisa. El análisis de imágenes es una técnica que permite a los científicos estudiar objetos de interés como características en una imagen digital y junto con el microscopio, examinar objetos en una amplia gama de escalas. Los objetivos del presente trabajo fueron: 1) Cuantificar a las esporas e hifas a través de la micro-cartografía de secciones delgadas de diferentes tipos de suelos; 2) Determinar su patrón de distribución referida con respecto a poros, agregados y residuos orgánicos durante el proceso de mineralización de residuos orgánicos en...
Palavras-chave: Ustifluvents; Micro-cartografía; Análisis de imágenes; Micromorfología; Clasificación supervisada; Micro-mapping; Image analysis; Micromorphologi; Supervised classification; Edafología; Maestría.
Ano: 2011 URL: http://hdl.handle.net/10521/577
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Microcartografía y patrones espectrales de la actividad biológica en secciones delgadas. Colegio de Postgraduados
Suástegui Méndez, Enrique.
Los estudios sobre la cuantificación de hongos se realizan de manera alterada, por lo que poco se conoce sobre la distribución de las esporas e hifas en el suelo y sus relaciones con rasgos edáficos de manera precisa. El análisis de imágenes es una técnica que permite a los científicos estudiar objetos de interés como características en una imagen digital y junto con el microscopio, examinar objetos en una amplia gama de escalas. Los objetivos del presente trabajo fueron: 1) Cuantificar a las esporas e hifas a través de la micro-cartografía de secciones delgadas de diferentes tipos de suelos; 2) Determinar su patrón de distribución referida con respecto a poros, agregados y residuos orgánicos durante el proceso de mineralización de residuos orgánicos en...
Palavras-chave: Ustifluvents; Micro-cartografía; Análisis de imágenes; Micromorfología; Clasificación supervisada; Micro-mapping; Image analysis; Micromorphologi; Supervised classification; Edafología; Maestría.
Ano: 2011 URL: http://hdl.handle.net/10521/577
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SELECTION OF EFFECTIVE SOFTWARE FOR THE ANALYSIS OF THE FRACTIONAL COMPOSITION OF THE CHAOTIC SEED LAYER USING THE EXAMPLE OF OILSEED RADISH REA
Tsytsiura,Yaroslav.
ABSTRACT This article generalises the peculiarities and efficiency of the use of three software packages, Digimizer image analysis software (v. 4.2), ImageJ2 version 2.0.0-rc-64 and WipFrag 3.3.14.0. These packages were used in studying the fractional composition of oilseed radish seeds for scanning in the mode of single-layer inseparable placement. The generalisation of the main caveats and features of the effective use of each of the software packages is made on the basis of detailed analysis of the features of each of package in the use to the features of the scan and the obtained scanned image The above-mentioned aspects are illustrated in detail and reservations about the options for the effective use of each package are represented. The efficiency...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fractional composition; Image analysis; Oilseed radish; Scanning; Seeds.
Ano: 2021 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69162021000200161
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Digital surface area assessment of broiler chickens REA
Yanagi Júnior,Tadayuki; Silva,Éberson; Braga Júnior,Roberto A.; Lopes,Marcos A.; Damasceno,Flávio A.; Silva,Gleice C. de A. E.
Surface area (SA) of poultry is an important parameter for heat and mass transfer calculations. Optical approaches, such as the moiré technique (MT), are non-destructive, result in accuracy and speed gains, and preserve the object integrity. The objective of this research was to develop and validate a new protocol for estimating the surface area (SA) of broiler chickens based on the MT. Sixty-six Ross breed broiler chickens (twenty-seven male, thirty-nine female, ages spanning all growth phases) were used in this study. The dimensions (length, width and height) and body mass of randomly selected broiler chickens were evaluated in the laboratory. Chickens were illuminated by a light source, and grids were projected onto the chickens to allow their shape to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Image analysis; Moiré technique; Poultry.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69162011000300007
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