SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

MAHROSE,KHALID M.; ELHACK,MOHAMED E. ABD; MAHGOUB,SAMIR A.; ATTIA,FATEN A. M..

Abstract: This study investigated the effects of stocking density and dietary supplementation of probiotic (Lactobacillus) on growth, traits of carcass, lymphoid organs and intestinal microbial count of Japanese quail. Quail chicks (one week old) were randomly allotted to 6 groups in a 2×3 factorial experiment included 2 levels of stocking density (100, and 75 cm2/bird) and 3 levels of probiotic (0.00, 0.02 and 0.04 g/kg diet). Quail reared at 100 cm2/ bird showed significantly the highest body weight and daily gain. Quail reared at 75 cm2/ bird had significantly the lower average of feed consumption and the best ratio of feed conversion. Increasing level of probiotic up to 0.04 g/kg led to a significant decrease in dressing percentage by about 2.7 %...

Generation of a triple-fluorescent mouse strain allows a dynamic and spatial visualization of different liver phagocytes in vivo

Palavras-chave: Stocking density; Probiotic; Growth; Immunology; Microbial count; Quail.

Ano: 2019

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000300603

NAKAGAKI,BRENDA N.; FREITAS-LOPES,MARIA A.; CARVALHO,ÉRIKA; CARVALHO-GONTIJO,RAQUEL; CASTRO-OLIVEIRA,HORTÊNCIA M.; REZENDE,RAFAEL M.; CARA,DENISE C.; SANTOS,MÔNICA M.; LOPES,RODRIGO PESTANA; DAVID,BRUNA A.; MENEZES,GUSTAVO B..

ABSTRACT Resident and circulating immune cells have been extensively studied due to their almost ubiquitous role in cell biology. Despite their classification under the “immune cell department”, it is becoming increasingly clear that these cells are involved in many different non-immune related phenomena, including fetus development, vascular formation, memory, social behavior and many other phenotypes. There is a huge potential in combining high-throughput assays - including flow cytometry and gene analysis - with in vivo imaging. This can improve our knowledge in both basic and clinical cell biology, and accessing the expression of markers that are relevant in the context of both homeostasis and disease conditions might be instrumental. Here we describe...

Immunology and immunopathology of African trypanosomiasis

Palavras-chave: Intravital microscopy; Phagocytes; Liver immunology; Fluorescent markers; Hepatology; Gastroenterology; Immunology.

Ano: 2019

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000200601

Vincendeau,Philippe; Bouteille,Bernard.

Major modifications of immune system have been observed in African trypanosomiasis. These immune reactions do not lead to protection and are also involved in immunopathology disorders. The major surface component (variable surface glycoprotein,VSG) is associated with escape to immune reactions, cytokine network dysfunctions and autoantibody production. Most of our knowledge result from experimental trypanosomiasis. Innate resistance elements have been characterised. In infected mice, VSG preferentially stimulates a Th 1-cell subset. A response of <FONT FACE=Symbol>gd</FONT> and CD8 T cells to trypanosome antigens was observed in trypanotolerant cattle. An increase in CD5 B cells, responsible for most serum IgM and production of autoantibodies...

Activity of Brazilian propolis against Aeromonas hydrophila and its effect on Nile tilapia growth, hematological and non-specific immune response under bacterial infection

Palavras-chave: Trypanosome; African trypanosomiasis; Immunology; Macrophage; Lymphocytes; Nitric oxide; Cytokine; Autoantibodies.

Ano: 2006

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652006000400004

ORSI,RICARDO O.; SANTOS,VIVIAN G. DOS; PEZZATO,LUIZ E.; CARVALHO,PEDRO L.P.F. DE; TEIXEIRA,CAROLINE P.; FREITAS,JAKELINE M.A.; PADOVANI,CARLOS R.; SARTORI,MARIA M.P.; BARROS,MARGARIDA M..

ABSTRACT The effect of the ethanolic extract of propolis (EEP) on Aeromonas hydrophila was analyzed by determination of minimum inhibitory concentration (MIC). Then, the effects of crude propolis powder (CPP) on growth, hemato-immune parameters of the Nile tilapia, as well as its effects on resistance to A. hydrophila challenge were investigated. The CPP (0.5, 1.0, 1.5, 2.0, 2.5 and 3.0%) was added to the diet of 280 Nile tilapia (50.0 ± 5.7 g fish-1). Hemato-immune parameters were analyzed before and after the bacterial challenge. Red blood cell, hematocrit, hemoglobin, mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), and hydrogen peroxide (H2O2) and nitric oxide (NO) were evaluated. The MIC of the EEP was 13% (v/v) with a...

Morphometric analysis of the thymus of puppies infected with the Snyder Hill Strain of canine distemper virus

Palavras-chave: Health; Hematology; Immunology; Oreochromis niloticus; Propolis; Stress.

Ano: 2017

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000401785

Arq. Bras. Med. Vet. Zootec.

Alves,C.M.; Vasconcelos,A.C.; Martins,A.S.; del Puerto,H.L.; Santos,F.G.A.; Nunes,J.E.S.; Campos,P.P.; Moro,L..

The thymic morphometry analysis was used for determining apoptosis and atrophy of the thymus of eight puppies inoculated with canine distemper virus (CDV). Three healthy dogs were used as negative controls. Sections, 5µm thick, were stained by HE and Shorr, and the latter were evaluated by morphometry. CDV nucleoprotein was detected by immunohistochemistry. Morphometric results confirmed lymphoid hypotrophy in CDV inoculated dog thymuses, more stroma, less parenchyma and higher apoptotic index/field than negative control (not inoculated) puppies. Apoptosis plays a role in the mechanism of thymus atrophy that develops in canine distemper.

Evaluation of different adjuvants formulations for bluetongue vaccine

Palavras-chave: Puppy; Apoptosis; Thymus; Canine distemper; Immunology; Virus; Immunosuppression.

Ano: 2006

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000400004

BABT

Macedo,Ludmila Branco; Lobato,Zélia Inês Portela; Fialho,Sílvia Ligório; Viott,Aline de Marco; Guedes,Roberto Maurício Carvalho; Silva-Cunha,Armando.

This study investigated the adjuvant potential of W/O/W multiple emulsions and microemulsions, comparing them with traditional aluminum hydroxide and oil-in-water emulsion adjuvants against bluetongue vaccine (BTV). Local inflammatory reactions were assessed in rabbits by measuring the temperature of the animals and the skin thickness at the site of application. Antibodies titers were determined by serum-neutralization test. Histological analyses of lesions at the site of adjuvants application were done. Results showed that multiple emulsion and microemulsion maintained their stability even in the presence of complex components and presented adequate characteristics for subcutaneous administration. They were able to induce immune response against BTV, but...

Immunization of hybrid surubim (Pseudoplatystoma corruscans x P. fasciatum) against Motile Aeromonas hydrophila septicemia

Palavras-chave: Emulsion; Microemulsion; Vaccine adjuvants; Immunology; Biocompatibility.

Ano: 2013

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600007

BABT

Silva,Bruno Correa da; Jatobá,Adolfo; Vieira,Felipe do Nascimento; Mouriño,José Luiz Pedreira; Bolívar,Norha; Seiffert,Walter Quadros; Martins,Mauricio Laterça.

The aim of this study was to evaluate the immune response of hybrid surubim vaccinated by intraperitoneal injection and by immersion against Aeromonas hydrophila. Vaccine was prepared with formalin-inactivated A. hydrophila. Forty eight fishes (47±9g) were distributed in 12 tanks (4 fish / tank) in the following treatments (4 tanks / treatment): unvaccinated fish, vaccinated intraperitoneally and vaccinated by immersion. After 21 days, intraperitoneally vaccinated fishes showed the highest values of total protein and immunoglobulins, agglutination titer and serum antimicrobial activity. Lysozyme concentration in the serum was higher in the fishes vaccinated by immersion than unvaccinated fishes. Intraperitoneal vaccination induced the highest immune...

Gut: Key Element on Immune System Regulation

Palavras-chave: Pseudoplatystoma; Aeromonas hydrophila; Vaccine; Immunology.

Ano: 2013

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000100010

BABT

Delgobo,Murilo; Paludo,Katia Sabrina; Fernandes,Daniel; Oliveira,Junior Garcia de; Ortolan,Gilberto Luiz; Favero,Giovani Marino.

Abstract The gut is the main organ that mediates the contact between antigens with our organism, controlling the immune response against environmental factors, such as microbiota and food. Innate lymphoid cells participate in the gut-associated lymphoid tissue (GALT) maturation during the prenatal and early postnatal periods. After birth, breast milk provides the essential elements for the continuity of development of this tissue, leading to structural changes and healthy microbiota installation. The microbiota participates in the organogenesis of the GALT, as in the formation of intestinal villi, stimulating the proliferation of stem cells and maintaining the integrity of epithelial barrier. Foods are also involved in maturation of the GALT, where the...

CCR3 antagonist impairs estradiol-induced eosinophil migration to the uterus in ovariectomized mice

Palavras-chave: Immunology; Gut; Peripheral Tolerance.

Ano: 2019

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100901

BJMBR

Araújo,J.M.D.; Silva,L.A.S.; Felix,F.B.; Camargo,E.A.; Grespan,R..

Eosinophils are abundant in the reproductive tract, contributing to the remodeling and successful implantation of the embryo. However, the mechanisms by which eosinophils migrate into the uterus and their relationship to edema are still not entirely clear, since there are a variety of chemotactic factors that can cause migration of these cells. Therefore, to evaluate the role of CCR3 in eosinophil migration, ovariectomized C57BL/6 mice were treated with CCR3 antagonist SB 328437 and 17β-estradiol. The hypothesis that the CCR3 receptor plays an important role in eosinophil migration to the mouse uterus was confirmed, because we observed reduction in eosinophil peroxidase activity in these antagonist-treated uteruses. The antagonist also influenced uterine...

Cytokine production profile of heart-infiltrating T cells in Chagas' disease cardiomyopathy

Palavras-chave: Estradiol; Chemokine receptor; Immunology; Menopause; Uterus.

Ano: 2020

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000100603

BJMBR

Cunha-Neto,E.; Rizzo,L.V.; Albuquerque,F.; Abel,L.; Guilherme,L.; Bocchi,E.; Bacal,F.; Carrara,D.; Ianni,B.; Mady,C.; Kalil,J..

The hallmark of chronic Chagas' disease cardiomyopathy (CCC) is the finding of a T cell-rich inflammatory mononuclear cell infiltrate in the presence of extremely few parasites in the heart lesions. The scarcity of parasites in affected heart tissue casts doubt on the direct participation of Trypanosoma cruzi in CCC heart tissue lesions, and suggests the possible involvement of autoimmunity. The cells in the infiltrate are presumably the ultimate effectors of tissue damage, and there is evidence that such cells recognize cardiac myosin in molecular mimicry with T. cruzi proteins rather than primary reactivity to T. cruzi antigens (Cunha-Neto et al. (1996) Journal of Clinical Investigation, 98: 1709-1712). Recently, we have studied heart-infiltrating T...

Desenvolvimento de ensaios de imunoadsorção enzimática baseados em MSP1 e MSP2 recombinantes de isolado brasileiro de Anaplasma marginale.

Palavras-chave: Trypanosoma cruzi; Chagas' disease cardiomyopathy; Immunology; Cytokines; Gamma-interferon.

Ano: 1998

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100018

Infoteca-e

ARAÚJO, F. R. de; MELO, V. S. P.; RAMOS, C. A. do N.; MADRUGA, C. R.; SOARES, C. O.; KESSLER, R. H.; ALMEIDA, N. F. de; ARAÚJO, G. S. de; ALVES, L. C.; TORRES JÚNIOR, R. A. de A.; FRAGOSO, S. P.; ARAUCO, P. R. C.; BACENELLI, G.; OLIVEIRA, M. B de.

Ensaios de imunoadsorção indireta (ELISAs) baseados em MSP1a e MSP2 recombinantes de isolado brasileiro de Anaplasma marginale foram desenvolvidos para detecção de anticorpos contra esta requétsia em bovinos. As altas sensibilidades(99% para ambos os testes) e especificidades (100% para ambros os ensaios) foram confirmadas pela análise de soros positivos ou negativos para anticorpos contra A.marginale, respectivamente, por imunofluorescência indireta. Pela análise de 583 amostras de soros de bovinos de três mesorregiões do Estado de Pernambuco, a concordância entre os dois testes foi alta, com índice kappa de 0,89. As prevalências de bovinos soropositivos também foram determinadas pelos dois ensaios, os quais definiram as mesorregiões de Recife e Zona da...

Tipo: Folhetos

Palavras-chave: Técnica imuoenzimática; Brasil; Immunoenzyme techniques; Anaplasma Marginale; Elisa; Imunologia; Brazil; Immunology.

Ano: 2004

URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/318268

Vacinação e fatores que influenciam a eficácia da vacinação nas aves.

Infoteca-e

BASTOS, A. P. A.; CARON, L..

Neste artigo, os autores fornecem uma visão sobre o uso de vacinação para o controle de infecções de aves em qualquer região e tipo de produção, com particular ênfase para o controle das doenças aviárias.

Tipo: Artigo na mídia

Palavras-chave: Ave; Imunologia; Vacinação; Avicultura; Immunology; Vaccination; Poultry; Aviculture.

Ano: 2015

URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1031533

Prophylactic strategies and zootechnic measures, recent advances

Fixation d''un anticorps anti-lysozyme dans des cellules de mollusques bivalves

Grizel, Henri.

The spread of major diseases of molluscs around the world and the increase of transfers need the development of adapted prophylactic strategies and the increase of research in epidemiology, immunology and genetics. The prophylactic strategies must be based on common laws with the establishment of a list of declarable pathogens, with basic sampling rules (sample size, frequency, definition of geographical areas) and with reglementation of the internal transfers. Moreover, specific adapted diagnosis should be developed to increase the sanitary control performance (e.g. ELISA test, DNA probe, cell culture). In the case of an infectious disease the epidemiology studies should be concerned, the relationship between the culture techniques and the disease, the...

Tipo: Text

Palavras-chave: Bonamia ostreae; Mollusc culture; Aquaculture techniques; Pathology; Immunology; Genetics; Epidemiology; Fish diseases; Disease control.

Ano: 1989

URL: http://archimer.ifremer.fr/doc/1989/acte-1483.pdf

Biological, physiological, immunological and nutritional assessment of farm-reared Litopenaeus stylirostris shrimp affected or unaffected by vibriosis

Auffret, Mathieu; Diouris, M; Herry, A.

A polyclonal rabbit anti-human lysozyme (Dako) was applied on histological sections from bivalve mollusc and revealed with a mouse anti-rabbit IgG fluorescein conjugated antibody to localise cellular lysozyme in tissues where its activity had previously detected by enzymatic techniques. Labelling occurred in the cytoplasm of epithelial cells covering the body. This is in agreement with the intracellular distribution of this enzyme. In the Lucinid Lucinella divaricata gills, the cytoplasm of bacteriocytes was also labelled. This suggests that antibacterial activity occurred in these cells. Control sections indicate that fluorescence was a consequence of true primary antibody fixation. However, its specificity should be verified by electrophoretical...

Tipo: Text

Palavras-chave: Bivalvia; Immunology; Antibodies; Electrophoresis; Histology.

Ano: 1991

URL: http://archimer.ifremer.fr/doc/1991/acte-1723.pdf

Strategy of researches on marine bivalve defense mechanisms

Mugnier, Chantal; Justou, Carole; Lemonnier, Hugues; Patrois, Jacques; Ansquer, Dominique; Goarant, Cyrille; Le Coz, Jean-rene.

Shrimp aquaculture in New Caledonia is subject to seasonal mortalities during grow-out due to highly virulent Vibrio nigripulchritudo (Vn). To understand the mechanisms affecting shrimp resistance and leading to significant mortality, a shrimp ecophysiology and immunology survey was conducted on two farms, the first considered as a "control" farm (HC), the second affected by the disease (DF). Mortality observed during the survey at DF started 50 days after stocking and was typical of this disease. The main observations regarding shrimp were: (a) growth was not affected by the disease and was faster in the DF than in the HC pond; (b) disease did not affect one sex more than the other, or a specific part of the population in terms of weight; (c) the physical...

Tipo: Text

Palavras-chave: Vibriosis; Penaeid shrimp; Field survey; Physiology; Immunology; Nutritional status.

Ano: 2013

URL: http://archimer.ifremer.fr/doc/00176/28764/27567.pdf

Physiology and immunity of the invasive giant African snail, Achatina (Lissachatina) fulica, intermediate host of Angiostrongylus cantonensis

Bachere, Evelyne; Mialhe, Eric; Noel, Thierry; Boulo, Viviane; Hervio, Dominique; Le Gall, Ghyslaine; Morvan, Annie.

Because of the impact of infectious diseases in marine mollusc aquaculture, research has been initially developed in pathology in term of epidemiology and diagnosis. The establishment of basic techniques for in vitro and in vivo studies of host/pathogen interactions and the progresses in the field of cellular and molecular biology have permitted to undertake researches in immunology. Parallel to fundamental interest in understanding defense mecanisms of molluscs against pathogens, applications may be expected for resistant strains obtained by genetic engineering.

Tipo: Text

Palavras-chave: Defense mechanisms; Hosts; Immunology; Pathogens; Crassostrea gigas; Mytilus edulis.

Ano: 1991

URL: http://archimer.ifremer.fr/doc/00038/14884/12207.pdf

Lima, Mariana G.; De C. Augusto, Ronaldo; Pinheiro Da Silva, Jairo; Thiengo, Silvana C..

As one of the most successful invasive land snail species, Achatina (Lissachatina) fulica Bowdich, 1822 has achieved wide global distribution, particularly in (sub)tropical regions, with further dispersal likely due to climate change. This species of giant African snails (up to 17 cm shell length) is a pest that has extensive negative impact on agriculture and can serve as vector for several parasites, including Angiostrongylus cantonensis, a nematode parasite that causes (human) eosinophilic meningitis, an emergent disease. Investigation showed that A. cantonensis infection negatively impacts the metabolism of A. fulica by depleting polysaccharide stores of the intermediate host, compromising the energy balance of the snail. A review of the literature...

Tipo: Text

Palavras-chave: Achatina fulica; Immunology; Physiology; Nematodes; Angiostrongylus cantonensis.

Ano: 2020

URL: https://archimer.ifremer.fr/doc/00599/71144/69462.pdf

Changes in biochemical and hemocyte parameters of the pacific oysters Crassostrea gigas fed T-Iso supplemented with lipid emulsions rich in eicosapentaenoic acid

Innovative rapid solid-phase immunobead assay for the detection of okadaic acid and related DSP toxins in shellfish

Delaporte, Maryse; Chu, Fu-lin; Langdon, Chris; Moal, Jeanne; Lambert, Christophe; Samain, Jean-francois; Soudant, Philippe.

The aim of this study was to assess the effect of dietary eicosapentaenoic acid (20:5n-3) on hemocyte parameters such as hemocyte concentration, phagocytosis, and non-stimulated reactive oxygen species (ROS) production in Pacific oysters Crassostrea gigas, as well on proximate biochemical and fatty acid compositions. One-year-old oysters (C. gigas) were fed T-Isochrysis aff. galbana(T-Iso), which is low in 20:5n-3, either alone or with supplements of a lipid emulsion rich in 20:5n-3 at 1%, 10% or 50% (dry weight of the algal ration) for up to 7 weeks. Changes in gill fatty acid composition demonstrated that the lipid emulsion was well ingested by oysters during the dietary conditioning. Biochemical analysis indicated that oysters fed supplements of 50%...

Tipo: Text

Palavras-chave: Polyunsaturated fatty acids; Immunology; Hemocytes; Eicosapentaenoic acid 20 : 5n 3; Crassostrea gigas; Bivalves.

Ano: 2007

URL: http://archimer.ifremer.fr/doc/2007/publication-2604.pdf